LOCUS LT727402 7171 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-mCASP-12, complete sequence.
ACCESSION LT727402
VERSION LT727402.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-mCASP-12
ORGANISM Mammalian expression vector pCAGGS-mCASP-12
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7171)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7171
/organism="Mammalian expression vector pCAGGS-mCASP-12"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3820."
/db_xref="taxon:1944877"
misc_feature 85..117
/note="5' UTR of mCASP-12"
CDS 118..1377
/transl_table=11
/note="mCASP-12"
/protein_id="SJL88353.1"
/translation="MAARRTHERDPIYKIKGLAKDMLDGVFDDLVEKNVLNGDELLKI
GESASFILNKAENLVENFLEKTDMAGKIFAGHIANSQEQLSLQFSNDEDDGPQKICTP
SSPSESKRKVEDDEMEVNAGLAHESHLMLTAPHGLQSSEVQDTLKLCPRDQFCKIKTE
RAKEIYPVMEKEGRTRLALIICNKKFDYLFDRDNADTDILNMQELLENLGYSVVLKEN
LTAQEMETELMQFAGRPEHQSSDSTFLVFMSHGILEGICGVKHRNKKPDVLHDDTIFK
IFNNSNCRSLRNKPKILIMQACRGRYNGTIWVSTNKGIATADTDEERVLSCKWNNSIT
KAHVETDFIAFKSSTPHNISWKVGKTGSLFISKLIDCFKKYCWCYHLEEIFRKVQHSF
EVPGELTQMPTIERVSMTRYFYLFPGN"
misc_feature 1378..2311
/note="3' UTR of mCASP-12"
regulatory 2461..2552
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 2553..2909
/note="3' FR of beta-globin"
regulatory complement(2987..3069)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 3211..3286
/note="SV40 ori"
regulatory 3317..3391
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3317..3391)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3506..3907)
/note="pMB1 ori"
CDS complement(4448..5308)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88354.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5444..5821
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5827..7168
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 6196..7120
/note="intron AG promoter"
BASE COUNT 1788 a 1698 c 1802 g 1883 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 ggatccccgg gaattcggca cgagggcacg agcggcacga gcggcacgag cacagccatg
121 gcggccagga ggacacatga aagagatcca atctacaaga tcaaaggttt ggccaaggac
181 atgctggatg gggtttttga tgacctggtg gagaagaatg ttttaaatgg agatgagtta
241 ctcaaaatag gggaaagtgc gagtttcatc ctgaacaagg ctgagaatct ggttgagaac
301 ttcttagaga aaacagacat ggcaggaaaa atatttgctg gccacattgc caattcccag
361 gaacagctga gtttacaatt ttctaatgat gaggatgatg gacctcagaa gatatgtaca
421 ccttcttctc catcagaatc caagagaaaa gtagaggatg atgaaatgga ggtaaatgct
481 ggattggccc atgaatcaca tctaatgctg acagctcctc atggactcca gagctcagaa
541 gtccaagata cactgaagct ttgtccacgt gatcagtttt gtaagataaa gacagaaagg
601 gcaaaagaga tatatccagt gatggagaag gagggacgaa cacgtctggc tctcatcatc
661 tgcaacaaaa agtttgacta cctttttgat agagataatg ctgatactga cattttgaac
721 atgcaagaac tacttgaaaa tcttggatac tctgtggtgt taaaggaaaa ccttacagct
781 caggaaatgg agacagagtt aatgcagttt gctggccgtc cagagcacca gtcctcagac
841 agcacattcc tggtgtttat gtcccatggc atcctggaag gaatctgtgg ggtgaagcac
901 cgaaacaaaa agccagatgt tcttcatgat gacactatct tcaaaatttt caacaactct
961 aactgtcgga gtctgagaaa caaacccaag attctcatca tgcaggcctg cagaggcaga
1021 tataatggaa ctatttgggt atccacaaac aaagggatag ccactgctga tacagatgag
1081 gaacgtgtgt tgagctgtaa atggaataat agtataacaa aggcccatgt ggagacagat
1141 ttcattgctt tcaaatcttc taccccacat aatatttctt ggaaggtagg caagactggt
1201 tccctcttca tttccaaact cattgactgc ttcaaaaagt actgttggtg ttatcatttg
1261 gaggaaattt ttcgaaaggt tcaacactca tttgaggtcc caggtgaact gacccagatg
1321 cccactattg agagagtatc catgacacgc tatttctacc tttttcccgg gaattagcac
1381 aggcaactct catgcagttc ccagtcaagt attgctgtag ctgagaagaa aagaaaattc
1441 caagatccca ggatttttaa atgtgtaaaa cttttcagtt agatatttta tacaatatca
1501 taaggctaga taatttccat aggttatttt taggggaaac ctgatacgta aaattttgca
1561 tgaaaagtat agattcgttc cttccttcca aatctgctca tcttttactt tattttatta
1621 aatttatatt tgcacatttt tgtaaaatga ataaaggaaa aagacactaa agaacatgtc
1681 tgaacatatc ctggaacttc ttctttgtaa aatgaataaa ggaaaaagac actaaagaac
1741 aaagatgata caccttttga tgaaccactt tcacctataa attagtagta agaatttact
1801 cacaaggaag atcttatcta caatgtagct gagaaatgaa actgttgcat atgtaggtaa
1861 aactaatcat cttttaggaa taagataaag aggtttatgt ccactgtttg tctctggcat
1921 acatggtttt catgcctagt ggctacctac cattcctttc aatcaatttc ttaggatata
1981 attgacaaat ttatgctatg tttataattt ttctcttaaa tgcctttgta ataagatata
2041 ctcacacata cacatttaag attctgtcta ttagtgttat tgaaataaat cctagaaaca
2101 aaaattaaaa gtttaaagca ttaaagacaa ttttgagaca aaatattcta tgaaattcta
2161 tgcaaaattg aattcttcta caagtatgtg atgaagccca cctaccacta tttttgtcat
2221 cttgaggatg ttatatcatt atttttactt aactgagctc aaattaaaat ctttgtttat
2281 tctttgttct ataaaaaaaa aaaaaaaaaa actcgtgccg aattcgatct ctttttttgg
2341 gtttggtggg gtatcttcat catcgaatag atagttatat acatcatcca ttgtagtggt
2401 attaaacatc cctgtagtga ttccaaacgc gccaatgccc tggctcacaa ataccactga
2461 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
2521 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
2581 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
2641 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
2701 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
2761 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
2821 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
2881 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc
2941 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
3001 agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact cacattaatt
3061 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc
3121 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
3181 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
3241 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
3301 gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag
3361 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
3421 actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg
3481 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
3541 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
3601 acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
3661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
3721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
3781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
3841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
3901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
3961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
4021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
4081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
4141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
4201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
4261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
4321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
4381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
4441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
4501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
4561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
4621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
4681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
4741 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
4801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
4861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
4921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
4981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
5041 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
5101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
5161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
5221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
5281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
5341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
5401 taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg attattgact
5461 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
5521 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
5581 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
5641 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
5701 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
5761 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
5821 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
5881 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
5941 ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa
6001 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
6061 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc
6121 cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
6181 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
6241 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
6301 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
6361 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
6421 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
6481 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
6541 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
6601 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
6661 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
6721 gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
6781 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
6841 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
6901 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
6961 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
7021 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
7081 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
7141 tattgtgctg tctcatcatt ttggcaaaga a
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