LOCUS LT727398 4292 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pBluhCASP-6, complete sequence.
ACCESSION LT727398
VERSION LT727398.1
KEYWORDS .
SOURCE Vector pBluhCASP-6
ORGANISM Vector pBluhCASP-6
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4292)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4292
/organism="Vector pBluhCASP-6"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3814."
/db_xref="taxon:1945256"
rep_origin 4..459
/note="f1 ori"
regulatory 623..639
/note="T7"
/regulatory_class="promoter"
misc_feature complement(690..1139)
/note="3UTR"
CDS complement(1140..2021)
/transl_table=11
/note="hCASP6"
/protein_id="SJL88345.1"
/translation="MSSASGLRRGHPAGGEENMTETDAFYKREMFDPAEKYKMDHRRR
GIALIFNHERFFWHLTLPERRRTCADRDNLTRRFSDLGFEVKCFNDLKAEELLLKIHE
VSTVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHSLVGKPKIFII
QACRGNQHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTLPAGADFLMCYSVAEGYYS
HRETVNGSWYIQDLCEMLGKYGSSLEFTELLTLVNRKVSQRRVDFCKDPSAIGKKQVP
CFASMLTKKLHFFPKSN"
regulatory complement(2106..2122)
/note="T3"
/regulatory_class="promoter"
regulatory complement(2186..2269)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(2361..2763)
/note="pMB1 ori"
CDS complement(3304..4164)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88346.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1034 a 1076 c 1014 g 1168 t
ORIGIN
1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc
661 ccccctcgag tttttttttt ttttttttta aggcagttct ctgctaggca ttaaacttta
721 aaacatttga atcattagac cataatgctt caccctaacg atatttatat aaaaggaaga
781 gaaagacatt ttcttttttt ttgagacgga gtttcactcg ttgcccaggc tggagtgcaa
841 tggcgcaatg ctcggctcac cgcagcctcc acctcctggg ttcaagtgat tctcctgcct
901 cagccttcca agtagctggg attgcaggca tgcgccgcca tgcctagcta aatttttttt
961 tgcattttta gtagaggcgg ggcttctgcc atgttggtca ggctggtctc gaactcccga
1021 cctcaggtga tccgcccacc ttggactccc aaagtgctgg gattacaggt gtgagtaacc
1081 acgcctggct gagaaagcca ttttcaatac agagtgtgaa attagatagc ctctattaat
1141 taattagatt ttggaaagaa atgcagcttt ttagttagca ttgaggcaaa acagggaacc
1201 tgcttctttc caattgcact tgggtctttg caaaagtcca ctcggcgctg agaaactttc
1261 ctgttcacca gtgtgaggag ttctgtgaac tctaaggagg agccatattt tcccaacatc
1321 tcacacaaat cttgaatgta ccatgagccg ttcacagttt cccggtgaga ataatatcct
1381 tctgcaacag agtaacacat gaggaagtca gctccagcag gcagcgtgta aacggaggct
1441 gcatccacct cagttatgtt ggtgtccaac ttctctgtct gattatctac tacatccaaa
1501 ggaatgactg gcacatcgtg ctggtttccc cgacatgcct ggatgataaa tatcttgggt
1561 tttccaacca ggctgtgaca cttgtctcct ttgaacaagc cagttaatgt ctgaatttcg
1621 attttagcat catatgcata aatgtgattg ccttcgccat ggctcaggaa gacacacaca
1681 aagcaatcgg catctgcgtg gctaacagtt gacacctcat gaattttgag cagtagttct
1741 tctgctttaa gatcattaaa gcatttcact tcaaatccta gatctgaaaa cctgcgggta
1801 agattgtctc tatctgcgca ggtgcgccgc ctttctggca gtgttaagtg ccaaaagaac
1861 ctctcatgat tgaagattaa agcaattcct ctcctcctgt ggtccatttt gtacttttct
1921 gccggatcaa acatttctct tttatagaag gcatctgttt ctgtcatgtt ttcttcccca
1981 cctgccgggt gccccctgcg gagccccgag gccgagctca ttgcagctcg agaattcctg
2041 cagcccgggg gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg
2101 ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt
2161 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
2221 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
2281 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
2341 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
2401 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
2461 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
2521 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
2581 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
2641 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
2701 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
2761 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
2821 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
2881 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
2941 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
3001 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
3061 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
3121 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
3181 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
3241 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
3301 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
3361 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
3421 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
3481 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
3541 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
3601 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
3661 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
3721 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
3781 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
3841 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
3901 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
3961 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
4021 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
4081 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
4141 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
4201 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
4261 ggttccgcgc acatttcccc gaaaagtgcc ac
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