LOCUS LT727393 4425 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial expression vector pPLc236, complete sequence.
ACCESSION LT727393
VERSION LT727393.1
KEYWORDS .
SOURCE Bacterial expression vector pPLc236
ORGANISM Bacterial expression vector pPLc236
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4425)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4425
/organism="Bacterial expression vector pPLc236"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 379."
/db_xref="taxon:1944692"
rep_origin complement(2010..2415)
/note="pMB1 ori"
CDS complement(2953..3813)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88332.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4179..4309
/note="PL"
/regulatory_class="promoter"
BASE COUNT 1030 a 1213 c 1155 g 1027 t
ORIGIN
1 ttccggatcc ggccaagctt ggccaagctt ggccggatcc tctacgccgg acgcatcgtg
61 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
121 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
181 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
241 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
301 aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
361 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
421 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
481 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
541 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
601 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
661 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
721 aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc
781 ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca
841 tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg
901 cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta
961 acggattcac cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc
1021 aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc
1081 gcatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga
1141 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg
1201 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg
1261 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta
1321 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
1381 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
1441 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta
1501 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt
1561 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
1621 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
1681 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt
1741 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
1801 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
1861 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
1921 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
1981 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
2041 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
2101 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
2161 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
2221 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
2281 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
2341 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
2401 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
2461 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
2521 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
2581 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
2641 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
2701 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
2761 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
2821 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
2881 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
2941 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
3001 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
3061 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
3121 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
3181 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
3241 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
3301 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
3361 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
3421 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
3481 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
3541 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
3601 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
3661 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
3721 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
3781 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attatgtaag
3841 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
3901 tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
3961 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca
4021 actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg
4081 atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc
4141 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tctcacctac caaacaatgc
4201 ccccctgcaa aaaataaatt catataaaaa acatacagat aaccatctgc ggtgataaat
4261 tatctctggc ggtgttgaca taaataccac tggcggtgat actgagcaca tcagcaggac
4321 gcactgacca ccatgaaggt gacgctctta aaattaagcc ctgaagaagg gcagcattca
4381 aagcagaagg ctttggggtg tgtgatacga aacgaagcat tggaa
//