LOCUS LT727389 6256 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSEAPBhBCTNf2, complete sequence.
ACCESSION LT727389
VERSION LT727389.1
KEYWORDS .
SOURCE Mammalian expression vector pSEAPBhBCTNf2
ORGANISM Mammalian expression vector pSEAPBhBCTNf2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6256)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6256
/organism="Mammalian expression vector pSEAPBhBCTNf2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3784."
/db_xref="taxon:1945062"
regulatory 60..357
/note="human BCTN promoter region"
/regulatory_class="promoter"
misc_feature 358..496
/note="5' UTR of human BCTN"
CDS 515..2035
/transl_table=11
/note="human SEAP"
/protein_id="SJL88320.1"
/translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQ
PAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNV
DKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSV
GVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILG
GGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLD
PSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDH
GHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIF
GLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHA
GEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTDAAHPG"
CDS <2551..2625
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88321.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 2626..2692
/note="intron of SV40 small t-antigen"
misc_feature 2693..3244
/note="3' UTR of SV40 small t-antigen"
regulatory 3245..3341
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3245..3341)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3530..3932)
/note="pMB1 ori"
CDS complement(4473..5333)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88322.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 5465..5920
/note="f1 ori"
regulatory 6088..6184
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(6088..6184)
/note="SV40 polyA late"
/regulatory_class="terminator"
BASE COUNT 1518 a 1659 c 1603 g 1476 t
ORIGIN
1 cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc taattcgtcg
61 ggggcccggc ctccccgatg cagaccacag cgccctcacg ggctgccctc aggccgcgca
121 gcgggcagcc gccagccgtc accccgggga gcgtccgtgg ggtgcccagg caccccaccc
181 cggcccgggg cgctcagacg gcagcagact gctgggcggc gcggggacta ctttccaccg
241 ccccctcgcg ccccgcccct tgtcctcgcg cggcggaacg ctccgcgctg cgccggtggc
301 ggcaggatac agcggcttct gcgcgactta taagagctcc ttgtgcggcg ccattttaag
361 cctctcggtc tgtggcagca gcgttggccc ggccccggga gcggagagcg aggggaggcg
421 gagacggagg aaggtctgag gagcagcttc agtccccgcc gagccgccac cgcaggtcga
481 ggacggtcgg actcccgacc tcgaagcttc ctgcatgctg ctgctgctgc tgctgctggg
541 cctgaggcta cagctctccc tgggcatcat cccagttgag gaggagaacc cggacttctg
601 gaaccgcgag gcagccgagg ccctgggtgc cgccaagaag ctgcagcctg cacagacagc
661 cgccaagaac ctcatcatct tcctgggcga tgggatgggg gtgtctacgg tgacagctgc
721 caggatccta aaagggcaga agaaggacaa actggggcct gagatacccc tggccatgga
781 ccgcttccca tatgtggctc tgtccaagac atacaatgta gacaaacatg tgccagacag
841 tggagccaca gccacggcct acctgtgcgg ggtcaagggc aacttccaga ccattggctt
901 gagtgcagcc gcccgcttta accagtgcaa cacgacacgc ggcaacgagg tcatctccgt
961 gatgaatcgg gccaagaaag cagggaagtc agtgggagtg gtaaccacca cacgagtgca
1021 gcacgcctcg ccagccggca cctacgccca cacggtgaac cgcaactggt actcggacgc
1081 cgacgtgcct gcctcggccc gccaggaggg gtgccaggac atcgctacgc agctcatctc
1141 caacatggac attgacgtga tcctaggtgg aggccgaaag tacatgtttc gcatgggaac
1201 cccagaccct gagtacccag atgactacag ccaaggtggg accaggctgg acgggaagaa
1261 tctggtgcag gaatggctgg cgaagcgcca gggtgcccgg tatgtgtgga accgcactga
1321 gctcatgcag gcttccctgg acccgtctgt gacccatctc atgggtctct ttgagcctgg
1381 agacatgaaa tacgagatcc accgagactc cacactggac ccctccctga tggagatgac
1441 agaggctgcc ctgcgcctgc tgagcaggaa cccccgcggc ttcttcctct tcgtggaggg
1501 tggtcgcatc gaccatggtc atcatgaaag cagggcttac cgggcactga ctgagacgat
1561 catgttcgac gacgccattg agagggcggg ccagctcacc agcgaggagg acacgctgag
1621 cctcgtcact gccgaccact cccacgtctt ctccttcgga ggctaccccc tgcgagggag
1681 ctccatcttc gggctggccc ctggcaaggc ccgggacagg aaggcctaca cggtcctcct
1741 atacggaaac ggtccaggct atgtgctcaa ggacggcgcc cggccggatg ttaccgagag
1801 cgagagcggg agccccgagt atcggcagca gtcagcagtg cccctggacg aagagaccca
1861 cgcaggcgag gacgtggcgg tgttcgcgcg cggcccgcag gcgcacctgg ttcacggcgt
1921 gcaggagcag accttcatag cgcacgtcat ggccttcgcc gcctgcctgg agccctacac
1981 cgcctgcgac ctggcgcccc ccgccggcac caccgacgcc gcgcacccgg gttaacccgt
2041 ggtccccgcg ttgcttcctc tgctggccgg gacatcaggt ggcccccgct gaattggaat
2101 cgatattgtt acaacacccc aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg
2161 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
2221 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
2281 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
2341 gagagatcct cataaaggcc aagaagggcg gaaagtccaa attgtaaaat gtaactgtat
2401 tcagcgatga cgaaattctt agctattgta atactgcgat gagtggcagg gcggggcgta
2461 atttttttaa ggcagttatt ggtgccctta aacgcctggt gctacgcctg aataagtgat
2521 aataagcgga tgaatggcag aaattcgccg gatctttgtg aaggaacctt acttctgtgg
2581 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
2641 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
2701 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
2761 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
2821 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
2881 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
2941 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
3001 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
3061 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
3121 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
3181 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
3241 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
3301 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
3361 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgtcgaccg atgcccttga
3421 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
3481 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct
3541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
3601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
3661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
3721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
3781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
3841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
3901 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
3961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
4021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
4081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
4141 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
4201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
4261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
4321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
4381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
4441 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
4501 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
4561 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
4621 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
4681 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
4741 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
4801 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
4861 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
4921 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
4981 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
5041 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
5101 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
5161 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
5221 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
5281 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
5341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
5401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
5461 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
5521 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
5581 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
5641 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
5701 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
5761 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
5821 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
5881 tttaacgcga attttaacaa aatattaacg tttacaattt cccattcgcc attcaggctg
5941 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta
6001 ccatgataag taagtaatat taaggtacgt ggaggtttta cttgctttaa aaaacctccc
6061 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
6121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
6181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atggtactgt
6241 aactgagcta acataa
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