LOCUS LT727383 7278 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGSEhA20S565A, complete sequence. ACCESSION LT727383 VERSION LT727383.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGSEhA20S565A ORGANISM Mammalian expression vector pCAGGSEhA20S565A other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7278) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..7278 /organism="Mammalian expression vector pCAGGSEhA20S565A" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 3776." /db_xref="taxon:1944768" misc_feature 16..54 /note="E-tag" CDS 64..2436 /transl_table=11 /note="hA20m" /protein_id="SJL88307.1" /translation="MAEQVLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIHHFKTMH RYTLEMFRTCQFCPQFREIIHKALIDRNIQATLESQKKLNWCREVRKLVALKTNGDGN CLMHATSQYMWGVQDTDLVLRKALFSTLKETDTRNFKFRWQLESLKSQEFVETGLCYD TRNWNDEWDNLIKMASTDTPMARSGLQYNSLEEIHIFVLCNILRRPIIVISDKMLRSL ESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSHHFVPLVTLKDSGPEIRAVPLV NRDRGRFEDLKVHFLTDPENEMKEKLLKEYLMVIEIPVQGWDHGTTHLINAAKLDEAN LPKEINLVDDYFELVQHEYKKWQENSEQGRREGHAQNPMEPSVPQLSLMDVKCETPNC PFFMSVNTQPLCHECSERRQKNQNKLPKLNSKPGPEGLPGMALGASRGEAYEPLAWNP EESTGGPHSAPPTAPSPFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHNARQLHASH APDHTRHLDPGKCQACLQDVTRTFNGICSTCFKRTTAEASSSLSTSLPPSCHQRSKAD PSRLVRSPSPHSCHRAGNDAPAGCLSQAARTPGDRTGTSKCRKAGCVYFGTPENKGFC TLCFIEYRENKHFAAASGKVSPTASRFQNTIPCLGRECGTLGSTMFEGYCQKCFIEAQ NQRFHEAKRTEEQLRSSQRRDVPRTTQSTSRPKCARASCKNILACRSEELCMECQHPN QRMGPGAHRGEPAPEDPPKQRCRAPACDHFGNAKCNGYCNECFQFKQMYG" misc_feature 2437..2480 /note="3UTR" regulatory 2568..2659 /note="polyA" /regulatory_class="terminator" regulatory complement(3094..3176) /note="lac" /regulatory_class="promoter" rep_origin 3318..3393 /note="SV40 ori" regulatory 3424..3498 /note="polyA" /regulatory_class="terminator" regulatory complement(3424..3498) /note="polyA" /regulatory_class="terminator" rep_origin complement(3613..4014) /note="pMB1 ori" CDS complement(4555..5415) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL88308.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 5551..5928 /note="hCMV" /regulatory_class="promoter" regulatory 5934..7275 /note="AG" /regulatory_class="promoter" misc_feature 6303..7227 /note="intron" BASE COUNT 1660 a 1953 c 1969 g 1696 t ORIGIN 1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 61 gcaatggctg aacaagtcct tcctcaggct ttgtatttga gcaatatgcg gaaagctgtg 121 aagatacggg agagaactcc agaagacatt tttaaaccta ctaatgggat cattcatcat 181 tttaaaacca tgcaccgata cacactggaa atgttcagaa cttgccagtt ttgtcctcag 241 tttcgggaga tcatccacaa agccctcatc gacagaaaca tccaggccac cctggaaagc 301 cagaagaaac tcaactggtg tcgagaagtc cggaagcttg tggcgctgaa aacgaacggt 361 gacggcaatt gcctcatgca tgccacttct cagtacatgt ggggcgttca ggacacagac 421 ttggtactga ggaaggcgct gttcagcacg ctcaaggaaa cagacacacg caactttaaa 481 ttccgctggc aactggagtc tctcaaatct caggaatttg ttgaaacggg gctttgctat 541 gatactcgga actggaatga tgaatgggac aatcttatca aaatggcttc cacagacaca 601 cccatggccc gaagtggact tcagtacaac tcactggaag aaatacacat atttgtcctt 661 tgcaacatcc tcagaaggcc aatcattgtc atttcagaca aaatgctaag aagtttggaa 721 tcaggttcca atttcgcccc tttgaaagtg ggtggaattt acttgcctct ccactggcct 781 gcccaggaat gctacagata ccccattgtt ctcggctatg acagccatca ttttgtaccc 841 ttggtgaccc tgaaggacag tgggcctgaa atccgagctg ttccacttgt taacagagac 901 cggggaagat ttgaagactt aaaagttcac tttttgacag atcctgaaaa tgagatgaag 961 gagaagctct taaaagagta cttaatggtg atagaaatcc ccgtccaagg ctgggaccat 1021 ggcacaactc atctcatcaa tgccgcaaag ttggatgaag ctaacttacc aaaagaaatc 1081 aatctggtag atgattactt tgaacttgtt cagcatgagt acaagaaatg gcaggaaaac 1141 agcgagcagg ggaggagaga ggggcacgcc cagaatccca tggaaccttc cgtgccccag 1201 ctttctctca tggatgtaaa atgtgaaacg cccaactgcc ccttcttcat gtctgtgaac 1261 acccagcctt tatgccatga gtgctcagag aggcggcaaa agaatcaaaa caaactccca 1321 aagctgaact ccaagccggg ccctgagggg ctccctggca tggcgctcgg ggcctctcgg 1381 ggagaagcct atgagccctt ggcgtggaac cctgaggagt ccactggggg gcctcattcg 1441 gccccaccga cagcacccag cccttttctg ttcagtgaga ccactgccat gaagtgcagg 1501 agccccggct gccccttcac actgaatgtg cagcacaacg gattttgtga acgttgccac 1561 aacgcccggc aacttcacgc cagccacgcc ccagaccaca caaggcactt ggatcccggg 1621 aagtgccaag cctgcctcca ggatgttacc aggacattta atgggatctg cagtacttgc 1681 ttcaaaagga ctacagcaga ggcctcctcc agcctcagca ccagcctccc tccttcctgt 1741 caccagcgat cgaaggcaga tccctcgcgg ctcgtccgga gcccctcccc gcattcttgc 1801 cacagagctg gaaacgacgc ccctgctggc tgcctgtctc aagctgcacg gactcctggg 1861 gacaggacgg ggacgagcaa gtgcagaaaa gccggctgcg tgtattttgg gactccagaa 1921 aacaagggct tttgcacact gtgtttcatc gagtacagag aaaacaaaca ttttgctgct 1981 gcctcaggga aagtcagtcc cacagcgtcc aggttccaga acaccattcc gtgcctgggg 2041 agggaatgcg gcacccttgg aagcaccatg tttgaaggat actgccagaa gtgtttcatt 2101 gaagctcaga atcagagatt tcatgaggcc aaaaggacag aagagcaact gagatcgagc 2161 cagcgcagag atgtgcctcg aaccacacaa agcacctcaa ggcccaagtg cgcccgggcc 2221 tcctgcaaga acatcctggc ctgccgcagc gaggagctct gcatggagtg tcagcatccc 2281 aaccagagga tgggccctgg ggcccaccgg ggtgagcctg cccccgaaga cccccccaag 2341 cagcgttgcc gggcccccgc ctgtgatcat tttggcaatg ccaagtgcaa cggctactgc 2401 aacgaatgct ttcagttcaa gcagatgtat ggctaaccgg aaacaggtgg gtcacctcct 2461 gcaagaagtg gggcctcgag gtcgaggaat tcactcctca ggtgcaggct gcctatcaga 2521 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct 2581 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa 2641 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga 2701 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa 2761 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata 2821 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga 2881 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac 2941 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc 3001 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg 3061 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 3121 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 3181 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc 3241 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 3301 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 3361 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 3421 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 3481 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 3541 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 3601 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga 3721 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 3781 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 3841 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 3901 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 3961 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 4021 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 4081 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 4141 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 4201 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 4261 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 4321 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 4381 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 4441 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 4501 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 4561 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 4621 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 4681 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 4741 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 4801 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 4861 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 4921 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 4981 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 5041 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 5101 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 5161 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 5221 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 5281 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 5341 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 5401 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 5461 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 5521 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt 5581 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta 5641 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga 5701 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt 5761 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta 5821 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg 5881 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga 5941 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt 6001 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc 6061 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag 6121 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 6181 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc 6241 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac 6301 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac 6361 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt 6421 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg 6481 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc 6541 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg 6601 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg 6661 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg 6721 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt 6781 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg 6841 aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat 6901 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg 6961 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc 7021 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc 7081 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg 7141 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat 7201 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct 7261 catcattttg gcaaagaa //