LOCUS LT727372 5825 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMa9hsGCB1, complete sequence.
ACCESSION LT727372
VERSION LT727372.1
KEYWORDS .
SOURCE Vector pMa9hsGCB1
ORGANISM Vector pMa9hsGCB1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5825)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5825
/organism="Vector pMa9hsGCB1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3673."
/db_xref="taxon:1945338"
regulatory 24..105
/note="fd terminator"
/regulatory_class="terminator"
regulatory 134..215
/note="fd terminator"
/regulatory_class="terminator"
rep_origin 313..768
/note="f1 ori"
rep_origin complement(772..1145)
/note="pMB1 ori"
CDS complement(1686..2546)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88264.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature complement(2861..3520)
/note="chloramphenicol sensitive"
misc_feature 3770..3820
/note="5' UTR of hsGCB1"
CDS 3821..5680
/transl_table=11
/note="hsGCB1"
/protein_id="SJL88265.1"
/translation="MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYD
DSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLD
ALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGT
EIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPYTFCKAFP
FHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVF
VLRSKEGLLDVEKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLT
RRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDT
LLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLL
NDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAG
QVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSE
YTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETKQDDD"
misc_feature 5681..5814
/note="3' UTR of hsGCB1"
BASE COUNT 1582 a 1365 c 1329 g 1549 t
ORIGIN
1 aattcacctc gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt
61 ttttttggag attttcaacg tgaaaaaatt attattcgca attccaagct aattcacctc
121 gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt ttttttggag
181 attttcaacg tgaaaaaatt attattcgca attccaagct ctgcctcgcg cgtttcggtg
241 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
301 cggatgcaga tcacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
361 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
421 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
481 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
541 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
601 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
661 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
721 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttga tctgcgctcg
781 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
841 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
1021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
1081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
1141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
1201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
1261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
1321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
1381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
1441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
1501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
1561 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
1621 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
1681 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
1741 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
1801 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
1861 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
1921 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
1981 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
2041 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
2101 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
2161 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
2221 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
2281 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
2341 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
2401 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
2461 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
2521 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcagacag
2581 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
2641 cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgactcccc gcgcgcgatg
2701 ggtcgaattt gctttcgaaa aaaaagcccg ctcattaggc gggctaaaaa aaagcccgct
2761 cattaggcgg gctcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc
2821 aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
2881 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg
2941 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
3001 ccatagtgaa aacgggggcg aagaagttgt ccatattcgc cacgtttaaa tcaaaactgg
3061 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
3121 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
3181 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
3241 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
3301 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
3361 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctaa acggtctggt
3421 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
3481 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
3541 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
3601 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc
3661 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta
3721 tttattcgaa gacgaaaggg catcgcgcgc ggggaattcc cgatccgcgc ggccgtacct
3781 ctgcgtgggg gctgcctccc cggctcccgg tgcagacacc atgtacggat ttgtgaatca
3841 cgccctggag ttgctggtga tccgcaatta cggccccgag gtgtgggaag acatcaaaaa
3901 agaggcacag ttagatgaag aaggacagtt tcttgtcaga ataatatatg atgactccaa
3961 aacttatgat ttggttgctg ctgcaagcaa agtcctcaat ctcaatgctg gagaaatcct
4021 ccaaatgttt gggaagatgt ttttcgtctt ttgccaagaa tctggttatg atacaatctt
4081 gcgtgtcctg ggctctaatg tcagagaatt tctacagaac cttgatgctc tgcacgacca
4141 ccttgctacc atctacccag gaatgcgtgc accttccttt aggtgcactg atgcagaaaa
4201 gggcaaagga ctcattttgc actactactc agagagagaa ggacttcagg atattgtcat
4261 tggaatcatc aaaacagtgg cacaacaaat ccatggcact gaaatagaca tgaaggttat
4321 tcagcaaaga aatgaagaat gtgatcatac tcaattttta attgaagaaa aagagtcaaa
4381 agaagaggat ttttatgaag atcttgacag atttgaagaa aatggtaccc aggaatcacg
4441 catcagccca tatacattct gcaaagcttt tccttttcat ataatatttg accgggacct
4501 agtggtcact cagtgtggca atgctatata cagagttctc ccccagctcc agcctgggaa
4561 ttgcagcctt ctgtctgtct tctcgctggt tcgtcctcat attgatatta gtttccatgg
4621 gatcctttct cacatcaata ctgtttttgt attgagaagc aaggaaggat tgttggatgt
4681 ggagaaatta gaatgtgagg atgaactgac tgggactgag atcagctgct tacgtctcaa
4741 gggtcaaatg atctacttac ctgaagcaga tagcatactt tttctatgtt caccaagtgt
4801 catgaacctg gacgatttga caaggagagg gctgtatcta agtgacatcc ctctgcatga
4861 tgccacgcgc gatcttgttc ttttgggaga acaatttaga gaggaataca aactcaccca
4921 agaactggaa atcctcactg acaggctaca gctcacgtta agagccctgg aagatgaaaa
4981 gaaaaagaca gacacattgc tgtattctgt ccttcctccg tctgttgcca atgagctgcg
5041 gcacaagcgt ccagtgcctg ccaaaagata tgacaatgtg accatcctct ttagtggcat
5101 tgtgggcttc aatgctttct gtagcaagca tgcatctgga gaaggagcca tgaagatcgt
5161 caacctcctc aacgacctct acaccagatt tgacacactg actgattccc ggaaaaaccc
5221 atttgtttat aaggtggaga ctgttggtga caagtatatg acagtgagtg gtttaccaga
5281 gccatgcatt caccatgcac gatccatctg ccacctggcc ttggacatga tggaaattgc
5341 tggccaggtt caagtagatg gtgaatctgt tcagataaca atagggatac acactggaga
5401 ggtagttaca ggtgtcatag gacagcggat gcctcgatac tgtctttttg ggaatactgt
5461 caacctcaca agccgaacag aaaccacagg agaaaaggga aaaataaatg tgtctgaata
5521 tacatacaga tgtcttatgt ctccagaaaa ttcagatcca caattccact tggagcacag
5581 aggcccagtg tccatgaagg gcaaaaaaga accaatgcaa gtttggtttc tatccagaaa
5641 aaatacagga acagaggaaa caaagcagga tgatgactga atcttggatt atggggtgaa
5701 gaggagtaca gactaggttc cagttttctc ctaacacgtg ccaagcccag gagcagttct
5761 tccctatgga tacagatttt cttttgtcct tgtccattac cccaagactt tcttctagag
5821 gtcga
//