LOCUS LT727371 5856 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pATmIL6hIFNG, complete sequence.
ACCESSION LT727371
VERSION LT727371.1
KEYWORDS .
SOURCE Vector pATmIL6hIFNG
ORGANISM Vector pATmIL6hIFNG
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5856)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5856
/organism="Vector pATmIL6hIFNG"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3665."
/db_xref="taxon:1945250"
regulatory 42..519
/note="mIL6 promoter"
/regulatory_class="promoter"
CDS 600..1100
/transl_table=11
/note="hIFNG"
/protein_id="SJL88261.1"
/translation="MKYTSYILAFQLCIVLGSLGCYCQDPYVKEAENLKKYFNAGHSD
VADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNV
KFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFR
GRRASQ"
CDS <1429..1503
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88262.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 1504..1570
/note="intron of SV40 small t-antigen"
misc_feature 1571..2122
/note="3' UTR of SV40 small t-antigen"
regulatory 2123..2219
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2123..2219)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3547..3949)
/note="pMB1 ori"
CDS complement(4490..5350)
/transl_table=11
/note="ampicillin"
/protein_id="SJL88263.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5482..5556
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(5482..5556)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 5697..5793
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(5697..5793)
/note="SV40 polyA late"
/regulatory_class="terminator"
BASE COUNT 1534 a 1373 c 1328 g 1621 t
ORIGIN
1 aagcttgcat gcctgcaggt cgactctaga ggatccttac agacatacaa aagaatccta
61 gcctcttatt catgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgta
121 tgtgtgtgtc gtctgtcatg cgcgcgtgcc tgcgtttaaa taacatcagc tttacgttct
181 ctttctcctt ataaaacatt gtgaatttca gttttctttc ccatcaagac atgctcaagt
241 gctgagtcac ttttaaagaa aaaaagaaga gtgctcatgc ttcttagggc tagcctcaag
301 gatgacttaa gcacactttt cccttcctag ttgtgattct ttcgatgcta aacgacgtca
361 cattgtgcaa tcttaataag gtttccaatc agccccaccc actctggccc cacccccacc
421 ctccaacaaa gatttttatc aaatgtggga ttttcccatg agtctcaaaa ttagagagtt
481 gactcctaat aaatatgaga ctggggatgt ctgtagctca ttctgctctg ggatctcgag
541 gtcagcttga tacaagaact actgatttca acttctttgg cttaattctc tcggaaacga
601 tgaaatatac aagttatatc ttggcttttc agctctgcat cgttttgggt tctcttggct
661 gttactgcca ggacccatat gtaaaagaag cagaaaacct taagaaatat tttaatgcag
721 gtcattcaga tgtagcggat aatggaactc ttttcttagg cattttgaag aattggaaag
781 aggagagtga cagaaaaata atgcagagcc aaattgtctc cttttacttc aaacttttta
841 aaaactttaa agatgaccag agcatccaaa agagtgtgga gaccatcaag gaagacatga
901 atgtcaagtt tttcaatagc aacaaaaaga aacgagatga cttcgaaaag ctgactaatt
961 attcggtaac tgacttgaat gtccaacgca aagcaataca tgaactcatc caagtgatgg
1021 ctgaactgtc gccagcagct aaaacaggga agcgaaaaag gagtcagatg ctgtttcgag
1081 gtcgaagagc atcccagtaa tggttgtcct gcctgcaata tttgaatttt aaatctaaat
1141 ctatttatta atatttaaca ttatttatat ggggaatata tttttagact catcaatcaa
1201 ataagtattt ataatagcaa cttttgtgta atgaaaatga atatctatta atatatgtat
1261 tatttataat tcctatatcc tgtgactgtc tcacttaatc ctttgttttc tgactaatta
1321 ggcaaggcta tgtgattaca aggctttatc tcaggggcca actaggcagc caacctaagc
1381 aagacctagg cttttgcaaa aagctcaagc ttggctctag agtcgacgga tctttgtgaa
1441 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
1501 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
1561 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
1621 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
1681 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
1741 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
1801 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
1861 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
1921 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
1981 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
2041 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
2101 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
2161 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
2221 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
2281 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
2341 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
2401 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
2461 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
2521 tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc
2581 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
2641 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
2701 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
2761 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
2821 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
2881 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
2941 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
3001 cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt
3061 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc
3121 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg
3181 aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca atcaattctt
3241 gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat
3301 ctccagcagc cgcacgcggc gcatctcggg cagcgttggg tcctggccac gggtgcgcat
3361 gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca
3421 gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac
3481 ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa
3541 gtcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
3601 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
3661 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
3721 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
3781 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
3841 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
3901 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
3961 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
4021 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
4081 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
4141 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
4201 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
4261 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
4321 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
4381 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
4441 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
4501 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
4561 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
4621 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
4681 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
4741 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
4801 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
4861 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
4921 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
4981 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
5041 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
5101 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
5161 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
5221 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
5281 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
5341 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
5401 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
5461 ttccccgaaa agtgccacct gaacttgttt attgcagctt ataatggtta caaataaagc
5521 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
5581 tccaaactca tcaatgtatc ttatcatgtc tggatcataa tcagccatac cacatttgta
5641 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
5701 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
5761 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
5821 aaactcatca atgtatctta tcatgtctgg atctcg
//