LOCUS LT727368 3493 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial expression vector pPLc300, complete sequence.
ACCESSION LT727368
VERSION LT727368.1
KEYWORDS .
SOURCE Bacterial expression vector pPLc300
ORGANISM Bacterial expression vector pPLc300
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3493)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..3493
/organism="Bacterial expression vector pPLc300"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3658."
/db_xref="taxon:1944700"
regulatory 359..440
/note="fdT"
/regulatory_class="terminator"
regulatory 469..550
/note="fdT"
/regulatory_class="terminator"
rep_origin 648..1103
/note="f1 ori"
rep_origin complement(1107..1480)
/note="pMB1 ori"
CDS complement(2021..2881)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88256.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3247..3377
/note="PL"
/regulatory_class="promoter"
BASE COUNT 896 a 874 c 824 g 899 t
ORIGIN
1 ttcagcacct gcacatggga cgtcgacctg aggtaattat aacccgggcc ctatatatgg
61 atccaattgc aatgatcatc atgacagatc tgcgcgcgat cgatatcagc gctttaaatt
121 tgcgcatgct agctatagtt ctagaggtac cggttgttaa cgttagccgg ctacgtatac
181 tccggaatat taataggcct aggatgcata tggcggccgc ctgcagctgg cgccatcgat
241 acgcgtacgt cgcgaccgcg gacatgtaca gagctcgaga agtactagtg gccacgtggg
301 ccgtgcacct taagcttggc cgcgctagag gtcgaaattc acctcgaaag caagctgata
361 aaccgataca attaaaggct ccttttggag cctttttttt tggagatttt caacgtgaaa
421 aaattattat tcgcaattcc aagctaattc acctcgaaag caagctgata aaccgataca
481 attaaaggct ccttttggag cctttttttt tggagatttt caacgtgaaa aaattattat
541 tcgcaattcc aagctctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
601 gcagctcccg gagacggtca cagcttgtct gtaagcggat gcagatcacg cgccctgtag
661 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
721 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
781 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
841 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
901 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
961 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
1021 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
1081 caaaatatta acgtttacaa tttgatctgc gctcggtcgt tcggctgcgg cgagcggtat
1141 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
1201 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
1261 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
1321 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
1381 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
1441 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
1501 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
1561 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
1621 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
1681 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
1741 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
1801 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
1861 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
1921 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
1981 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
2041 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
2101 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
2161 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
2221 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
2281 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
2341 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
2401 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
2461 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
2521 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
2581 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
2641 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
2701 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
2761 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
2821 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
2881 tactcttcct ttttcaatat tatgtaagca gacagtttta ttgttcatga tgatatattt
2941 ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat
3001 cgaacttttg ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc agaccgttcc
3061 gtggcaaagc aaaagttcaa aatcaccaac tggtccacct acaacaaagc tctcatcaac
3121 cgtggctccc tcactttctg gctggatgat ggggcgattc aggcctggta tgagtcagca
3181 acaccttctt cacgaggcag acctcagcgc cggtgatgcc ggccacgatg cgtccggcgt
3241 agaggatctc tcacctacca aacaatgccc ccctgcaaaa aataaattca tataaaaaac
3301 atacagataa ccatctgcgg tgataaatta tctctggcgg tgttgacata aataccactg
3361 gcggtgatac tgagcacatc agcaggacgc actgaccacc atgaaggtga cgctcttaaa
3421 attaagccct gaagaagggc agcattcaaa gcagaaggct ttggggtgtg tgatacgaaa
3481 cgaagcattg gaa
//