LOCUS LT727367 8752 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMRS101, complete sequence.
ACCESSION LT727367
VERSION LT727367.1
KEYWORDS .
SOURCE Vector pMRS101
ORGANISM Vector pMRS101
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8752)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8752
/organism="Vector pMRS101"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3654."
/db_xref="taxon:1945350"
CDS 696..1499
/transl_table=11
/note="StrA"
/note="streptomycin resistance"
/protein_id="SJL88251.1"
/translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLL
ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
CDS 1499..>2304
/transl_table=11
/note="StrB"
/note="streptomycin resistance"
/protein_id="SJL88252.1"
/translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAA"
regulatory complement(2335..2351)
/note="T3 promoter"
/regulatory_class="promoter"
rep_origin complement(2357..2635)
/note="pMB1 ori"
CDS complement(3176..4036)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88253.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 4139..4526
/note="R6K ori"
rep_origin complement(5243..5353)
/note="RK2/RP4 oriT"
CDS 5767..6270
/transl_table=11
/note="insB"
/protein_id="SJL88254.1"
/translation="MPGNSPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMD
EQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDG
WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
LNIKHYQ"
CDS complement(6408..7829)
/transl_table=11
/note="sacB"
/protein_id="SJL88255.1"
/translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGIS
HITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHG
YHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQT
QEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIF
DGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEES
LFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIAS
NTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLN
KTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLN
IKGKKTSVVKDSILEQGQLTVNK"
BASE COUNT 2076 a 2131 c 2192 g 2353 t
ORIGIN
1 gtcgacggat ccccgggtac cataagtaga agcagcaacc caagtagctt taccagcatc
61 cggttcaacc agcatagtaa gaatcttact ggacatcggc agttcttcga acagtgcgcc
121 aactaccagc tctttctgca gttcattcag ggcaccggag aacctgcgtg caatccatct
181 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat ccatggatta cgcgttaacc
241 cgggcccgcg gatgcatatg atcttaaggc ctaggtctag agtctttgtt ttgacgccat
301 tagcgtacgt aacaatcctc gttaaaggac aaggacctga gcggaagtgt atcgtacagt
361 agacggagta tactagtata gtctatagtc cgtggaatta ttatatttat ctccgacgat
421 attctcatca gtgaaatcca ggggaattct catgtttgac agcttatcat cgataagctt
481 taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct
541 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
601 gttatgccgg tactgccggg cctcttgcgg gataactgca cattcgggat atttctctat
661 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
721 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
781 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
841 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
901 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
961 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
1021 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
1081 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
1141 acgaggacaa gagtacgccg ctgcacgatc ttttggctcg tgtcgaacga gagctaccgg
1201 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
1261 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
1321 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
1381 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
1441 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat
1501 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga
1561 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa
1621 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg
1681 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga
1741 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac
1801 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc
1861 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca
1921 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag
1981 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc
2041 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc
2101 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat
2161 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga
2221 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca
2281 acgcgatcta gctatcgcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
2341 gagggttaat tgcgcgcatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
2401 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
2461 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
2521 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
2581 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
2641 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
2701 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
2761 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
2821 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
2881 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
2941 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
3001 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
3061 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
3121 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
3181 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
3241 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
3301 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
3361 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
3421 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
3481 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
3541 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
3601 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
3661 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
3721 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
3781 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
3841 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
3901 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
3961 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
4021 aatgttgaat actcatactc ttcctttttc aatctagcta gagcggccgc gatcaagcag
4081 gtgcgacaga cgtcatacta gatatctaga tccggcccga tgcgtccggc gtagaggatc
4141 tgaagatcag cagttcaacc tgttgatagt acgtactaag ctctcatgtt tcacgtacta
4201 agctctcatg tttaacgtac taagctctca tgtttaacga actaaaccct catggctaac
4261 gtactaagct ctcatggcta acgtactaag ctctcatgtt tcacgtacta agctctcatg
4321 tttgaacaat aaaattaata taaatcagca acttaaatag cctctaaggt tttaagtttt
4381 ataagaaaaa aaagaatata taaggctttt aaagctttta aggtttaacg gttgtggaca
4441 acaagccagg gatgtaacgc actgagaagc ccttagagcc tctcaaagca attttcagtg
4501 acacaggaac acttaacggc tgacatggga attctactgt ttgggtgtat gagctatttg
4561 caaaggaaat tggtgatgac aaagctcggc gctatttgaa gaaaatcgac tatggcaacg
4621 ccgatccttt ttgtccggtg ttgggttgaa ggtgaagccg gtcggggccg cagcgggggc
4681 cggcttttca gccttgcccc cctgcttcgg ccgccgtggc tccggcgtct tgggtgccgg
4741 cgcgggttcc gcagccttgg cctgcggtgc gggcacatcg gcgggcttgg ccttgatgtg
4801 ccgcctggcg tgcgagcgga acgtctcgta ggagaacttg accttccccg tttcccgcat
4861 gtgctcccaa atggtgacga gcgcatagcc ggacgctaac gccgcctcga catccgccct
4921 caccgccagg aacgcaaccg cagcctcatc acgccggcgc ttcttggccg cgcgggattc
4981 aacccactcg gccagctcgt cggtgtagct ctttggcatc gtctctcgcc tgtcccctca
5041 gttcagtaat ttcctgcatt tgcctgtttc cagtcggtag atattccaca aaacagcagg
5101 gaagcagcgc ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt
5161 atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc
5221 ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg
5281 gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct
5341 gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg ctgatgaaac
5401 caagccaacc aggaagggca gcccacctat caaggtgtac tgccttccag acgaacgaag
5461 agcgattgag gaaaaggcgg cggcggccgg catgagcctg tcggcctacc tgctggccgt
5521 cggccagggc tacaaaatca cgggcgtcgt ggactatgag cacgtccgcg agctggcccg
5581 catcaatggc gacctgggcc gcctgggcgg cctgctgaaa ctctggctca ccgacgaccc
5641 gcgcacggcg cggttcggtg atgccacgat ccgggcaacg ttgttgccat tgctgcagtt
5701 cacttacacc gcttctcaac ccggtacgca ccataaaatc attgatatgg ccatgaatgg
5761 cgttggatgc cgggcaacag cccgcattat gggcgttggc ctcaacacga ttttacgtca
5821 cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac agccgggcag tgacgtcatc
5881 gtctgcgcgg aaatggacga acagtggggc tatgtcgggg ctaaatcgcg ccagcgctgg
5941 ctgttttacg cgtatgacag tctccggaag acggttgttg cgcacgtatt cggtgaacgc
6001 actatggcga cgctggggcg tcttatgagc ctgctgtcac cctttgacgt ggtgatatgg
6061 atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca cgtaatcagc
6121 aagcgatata cgcagcgaat tgagcggcat aacctgaatc tgaggcagca cctggcacgg
6181 ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg
6241 cattatctga acataaaaca ctatcaataa gttggagtca ttaccaaaag gttaggaata
6301 cggttagcca tttgcctgct tttatatagt tcatatggga ttcaccttta tgttgataag
6361 aaataaaaga aaatgccaat aggatatcgg cattttcttt tgcgttttta tttgttaact
6421 gttaattgtc cttgttcaag gatgctgtct ttgacaacag atgttttctt gcctttgatg
6481 ttcagcagga agcttggcgc aaacgttgat tgtttgtctg cgtagaatcc tctgtttgtc
6541 atatagcttg taatcacgac attgtttcct ttcgcttgag gtacagcgaa gtgtgagtaa
6601 gtaaaggtta catcgttagg atcaagatcc atttttaaca caaggccagt tttgttcagc
6661 ggcttgtatg ggccagttaa agaattagaa acataaccaa gcatgtaaat atcgttagac
6721 gtaatgccgt caatcgtcat ttttgatccg cgggagtcag tgaacaggta ccatttgccg
6781 ttcattttaa agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga tgcaatcagc
6841 ggtttcatca cttttttcag tgtgtaatca tcgtttagct caatcatacc gagagcgccg
6901 tttgctaact cagccgtgcg ttttttatcg ctttgcagaa gtttttgact ttcttgacgg
6961 aagaatgatg tgcttttgcc atagtatgct ttgttaaata aagattcttc gccttggtag
7021 ccatcttcag ttccagtgtt tgcttcaaat actaagtatt tgtggccttt atcttctacg
7081 tagtgaggat ctctcagcgt atggttgtcg cctgagctgt agttgccttc atcgatgaac
7141 tgctgtacat tttgatacgt ttttccgtca ccgtcaaaga ttgatttata atcctctaca
7201 ccgttgatgt tcaaagagct gtctgatgct gatacgttaa cttgtgcagt tgtcagtgtt
7261 tgtttgccgt aatgtttacc ggagaaatca gtgtagaata aacggatttt tccgtcagat
7321 gtaaatgtgg ctgaacctga ccattcttgt gtttggtctt ttaggataga atcatttgca
7381 tcgaatttgt cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc aatagaagtt
7441 tcgccgactt tttgatagaa catgtaaatc gatgtgtcat ccgcattttt aggatctccg
7501 gctaatgcaa agacgatgtg gtagccgtga tagtttgcga cagtgccgtc agcgttttgt
7561 aatggccagc tgtcccaaac gtccaggcct tttgcagaag agatattttt aattgtggac
7621 gaatcgaatt caggaacttg atatttttca tttttttgct gttcagggat ttgcagcata
7681 tcatggcgtg taatatggga aatgccgtat gtttccttat atggcttttg gttcgtttct
7741 ttcgcaaacg cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt taatactgtt
7801 gcttgttttg caaacttttt gatgttcatc gttcatgtct ccttttttat gtactgtgtt
7861 agcggtctgc ttcttccagc cctcctgttt gaagatggca agttagttac gcacaataaa
7921 aaaagaccta aaatatgtaa ggggtgacgc caaagtatac actttgccct ttacacattt
7981 taggtcttgc ctgctttatc agtaacaaac ccgcgcgatt tacttttcga cctcattcta
8041 ttagactctc gtttggattg caactggtct attttcctct tttgtttgat agaaaatcat
8101 aaaaggattt gcagactacg ggcctaaaga actaaaaaat ctatctgttt cttttcattc
8161 tctgtatttt ttatagtttc tgttgcatgg gcataaagtt gcctttttaa tcacaattca
8221 gaaaatatca taatatctca tttcactaaa taatagtgaa cggcaggtat atgtgatggg
8281 ttaaaaagga tcttaaggcc taggtctagc ttggctgcag gggggggggg gggacttcca
8341 ttgttcattc cacggacaaa aacagagaaa ggaaacgaca gaggccaaaa agcctcgctt
8401 tcagcacctg tcgtttcctt tcttttcaga gggtatttta aataaaaaca ttaagttatg
8461 acgaagaaga acggaaacgc cttaaaccgg aaaattttca taaatagcga aaacccgcga
8521 ggtcgccgcc ccgtaacctg tcggatcacc ggaaaggacc cgtaaagtga taatgattat
8581 catctacata tcacaacgtg cgtggaggcc atcaaaccac gtcaaataat caattatgac
8641 gcaggtatcg tattaattga tctgcatcaa cttaacgtaa aaacaacttc agacaataca
8701 aatcagcgac actgaatacg gggcaacctc atgtcccccc ccccccctgc ag
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