LOCUS LT727365 6928 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF-BOSbsscFvB1-2C11, complete
sequence.
ACCESSION LT727365
VERSION LT727365.1
KEYWORDS .
SOURCE Mammalian expression vector pEF-BOSbsscFvB1-2C11
ORGANISM Mammalian expression vector pEF-BOSbsscFvB1-2C11
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6928)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6928
/organism="Mammalian expression vector
pEF-BOSbsscFvB1-2C11"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3617."
/db_xref="taxon:1945011"
rep_origin 207..663
/note="M13-O"
regulatory complement(918..1484)
/note="polyA"
/regulatory_class="terminator"
CDS complement(1515..>2270)
/codon_start=1
/transl_table=11
/note="sFv"
/protein_id="SJL88243.1"
/translation="EVKLVESGGGLVQPGKSLKLSCEASGFTFSGYGMHWVRQAPGRG
LESVAYITSSSINIKYLDAVKGRFTVSRDNAKNLLFLQMNILKSEDTAMYYCARFDWD
KNYWGQGTMVTVSSRSGGGGSGGGGSGGGGSMDIQMTQSPSSLPASLGDRVTINCQAS
QDISNYLNWYQQKPGKAPKLLIYYTNKLADGVPSRFSGSGSGRDSSFTISSLESEDIG
SYYCQQYYNYPWTFGPGTKLEIKGSRSHHHHHH"
CDS complement(<2325..>3047)
/codon_start=1
/transl_table=11
/note="B1sFv"
/protein_id="SJL88244.1"
/translation="EVKLVESGGGLVKPGGSLKLSCAASGFAFSTYDMSWVRQTPEKR
LEWVATISRGGNYTYYPDSVKGRFTISRDNARNALYLQMGSLRSEDTALYYCASPNWG
GGYFDVWGAGTTVTVSSGGGGSGGGGSGGGGSDIVMTQSQKFMSTSVGDRVSITCKAS
QNVGTDVAWYQQKLGQSPKLLIDSTSSRYTGVPDRFTGSGSGTDFTLTISNVQSEDLA
DYFCQQYSNYPLTFGAGTKLELK"
misc_feature complement(3048..3104)
/note="sB1H"
regulatory complement(3193..4336)
/note="hEF1"
/regulatory_class="promoter"
misc_feature complement(3202..4140)
/note="intron"
CDS complement(<4377..4446)
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88245.1"
/translation="MDKVLNREESLQLMDLLGLERSA"
rep_origin complement(4485..4560)
/note="SV40 ori"
regulatory complement(4517..4688)
/note="SV40 early promoter"
/regulatory_class="promoter"
regulatory complement(4750..4833)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(4925..5327)
/note="pMB1 ori"
CDS complement(5868..6728)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88246.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1658 a 1899 c 1758 g 1613 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
241 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
301 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
361 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
421 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
481 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
541 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
601 caggattttc gcctgctggg gcaaaccagc gtggaccgaa ttttaacaaa atattaacgt
661 tttgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
781 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
841 ccagtcacga cgttgtaaaa cgacggccag tgaattcttt tttttttttt tttttttttt
901 tttttttttt tttttttgga ggcaaacact ttattatcct gaagttcagt tacaatgtga
961 cagtggaggg gacactggtg agtggggggt gaagaggtgg agggtgacag tggagcttcc
1021 tttcacacac aggcctgaca tgattcctcc tgtctgctcc ctcccacatc cccagtcccc
1081 atccagcaag gcaaatgtcc aggcacctgc ctggccctaa aaagagtcac agtcctgacc
1141 ctcgtggggg cagggcaggt gtcccgaggc cacccagaaa aacaggagac acgtcggtga
1201 tgttcgggag tcaaaccatg tcccaaaagt cttaagaaga aaaacgagat tccccagcaa
1261 attcgtggga ccccagggcc atgcctccca gctctggcca cctctgcttg tccaggggcc
1321 tcacagccaa ggggatgcag gggccgctgt gcagtcggct gaggccagag tgaggggtgc
1381 aaggacccag atggggaaca ctgctgttta aatattaaac agggatttct tgtctcccac
1441 gtgggagacc tgatactctc aagggtccca ggtggggacc ctcactctag agtcgatcga
1501 cctgcagccc aagcttagtg atggtgatgg tgatgagatc tggatccttt tatttccagc
1561 ttggtgccag gtccgaacgt ccacggatag ttataatact gttgacagta ataagatcca
1621 atatcttcgg attccaggct gctgatagtg aaagaagaat ctctcccaga accactgcca
1681 ctgaaccttg atgggactcc atctgccaat ttatttgtat aatagatcag gagcttagga
1741 gctttccctg gtttctgctg gtaccagttt aaataattgc taatgtcctg actggcctga
1801 caattgatag tgactctgtc tcccagggag gcaggcagtg atgatggaga ctgggtcatc
1861 tggatgtcca tcgatccgcc accgccagag ccacctccgc ctgaaccgcc tccaccagat
1921 cttgaggaga cggtgaccat ggttccttgg ccccagtaat ttttgtccca gtcgaatctt
1981 gcacagtagt acatggctgt gtcctcagac ttgagaatgt tcatttgtag aaacagtaag
2041 ttcttggcat tgtctctgga gacggtgaac cggcctttca cagcgtccaa gtatttgata
2101 ttaatactac tactagtaat gtatgcgacc gactccagcc ccctccctgg agcctggcgg
2161 acccagtgca tgccatagcc gctgaaggtg aatccagagg cctcacagga gagtttcagg
2221 gactttccag gctgcaccaa gcctccccca gactccacca gcttgacctc catcgatccg
2281 ccaccgccag agccacctcc gcctgaaccg cctccaccag atcctttcag ctccagcttg
2341 gtcccagcac cgaacgtgag aggatagttg ctatattgtt ggcagaaata atctgccagg
2401 tcttcagact gcacattgct gatggtgaga gtgaaatctg tcccagatcc actgcctgtg
2461 aagcgatcag ggactccagt gtaccgactg gatgtcgagt caatcagtag tttaggagat
2521 tgtcctagtt tctgttgata ccaggctaca tcagtaccca cattctgact ggccttgcag
2581 gtgatgctga ccctgtctcc tactgatgtg gacatgaatt tttgagactg ggtcatcaca
2641 atgtccgatc cgccaccgcc agagccacct ccgcctgaac cgcctccacc tgaggagaca
2701 gtgaccgtgg tccctgcgcc ccagacatcg aagtaccccc ccccccagtt aggacttgca
2761 cagtaataca aggccgtgtc ctcagacctc agactgccca tttgcaggta cagggcgttc
2821 ctggcattgt ctctggagat ggtgaatcgg cccttcacac tgtctggata gtaggtgtaa
2881 ttaccaccac gactaatggt tgcgacccac tccagcctct tctccggagt ctggcgaacc
2941 caagacatgt cataggtact gaaagcgaat ccagaggctg cacaggagag tttcagggac
3001 cctccaggct tcactaagcc tcccccagac tccaccagct tcacttcaca ctggacacct
3061 tttaaaatta ggacaaggaa aatcaagctc agcccaaagt tcatggtgag gggtccgtgt
3121 tcagtgctga tcactgaatg tgaggaactg ggaattcgag ctcgcccggg agtcgatcct
3181 ctagagaatt cctcacgaca cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg
3241 cttgagaatg aaccaagatc caaactcaaa aagggcaaat tccaaggaga attacatcaa
3301 gtgccaagct ggcctaactt cagtctccac ccactcagtg tggggaaact ccatcgcata
3361 aaacccctcc ccccaaccta aagacgacgt actccaaaag ctcgagaact aatcgaggtg
3421 cctggacggc gcccggtact ccgtggagtc acatgaagcg acggctgagg acggaaaggc
3481 ccttttcctt tgtgtgggtg actcacccgc ccgctctccc gagcgccgcg tcctccattt
3541 tgagctccct gcagcagggc cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga
3601 ccgggccagc cttgccgccc agggcggggc gatacacggc ggcgcgaggc caggcaccag
3661 agcaggccgg ccagcttgag actacccccg tccgattctc ggtggccgcg ctcgcaggcc
3721 ccgcctcgcc gaacatgtgc gctgggacgc acgggccccg tcgccgcccg cggccccaaa
3781 aaccgaaata ccagtgtgca gatcttggcc cgcatttaca agactatctt gccagaaaaa
3841 aagcgtcgca gcaggtcatc aaaaatttta aatggctaga gacttatcga aagcagcgag
3901 acaggcgcga aggtgccacc agattcgcac gcggcggccc cagcgcccag gccaggcctc
3961 aactcaagca cgaggcgaag gggctcctta agcgcaaggc ctcgaactct cccacccact
4021 tccaacccga agctcgggat caagaatcac gtactgcagc caggtggaag taattcaagg
4081 cacgcaaggg ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac
4141 ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat
4201 atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca
4261 gtttaccccg cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt
4321 ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc ctcacgaggc
4381 actcctttca agacctagaa ggtccattag ctgcaaagat tcctctctgt ttaaaacttt
4441 atccatcttt gcaaagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
4501 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
4561 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
4621 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
4681 gggagcctaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
4741 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
4801 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
4861 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
4921 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
4981 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
5041 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
5101 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
5161 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
5221 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
5281 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
5341 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
5401 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
5461 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
5521 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
5581 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
5641 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
5701 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
5761 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
5821 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
5881 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
5941 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
6001 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
6061 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
6121 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
6181 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
6241 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
6301 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
6361 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
6421 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
6481 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
6541 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
6601 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
6661 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
6721 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
6781 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
6841 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
6901 aataggcgta tcacgaggcc ctttcgtc
//