LOCUS LT727363 9700 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV51B1sFv, complete sequence.
ACCESSION LT727363
VERSION LT727363.1
KEYWORDS .
SOURCE Mammalian expression vector pSV51B1sFv
ORGANISM Mammalian expression vector pSV51B1sFv
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9700)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9700
/organism="Mammalian expression vector pSV51B1sFv"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3608."
/db_xref="taxon:1945100"
regulatory 101..382
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 339..414
/note="SV40 ori"
misc_feature 453..698
/note="SV40 large T-antigen"
CDS 453..977
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL88236.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
misc_feature 699..1044
/note="intron"
misc_feature 1045..2925
/note="SV40 large T-antigen"
regulatory complement(2926..3022)
/note="polyA"
/regulatory_class="terminator"
regulatory 2926..3022
/note="polyA"
/regulatory_class="terminator"
regulatory complement(3096..3191)
/note="tO"
/regulatory_class="terminator"
CDS complement(3211..>3960)
/codon_start=1
/transl_table=11
/note="B1sFv"
/protein_id="SJL88237.1"
/translation="EVKLVESGGGLVKPGGSLKLSCAASGFAFSTYDMSWVRQTPEKR
LEWVATISRGGNYTYYPDSVKGRFTISRDNARNALYLQMGSLRSEDTALYYCASPNWG
GGYFDVWGAGTTVTVSSGGGGSGGGGSGGGGSDIVMTQSQKFMSTSVGDRVSITCKAS
QNVGTDVAWYQQKLGQSPKLLIDSTSSRYTGVPDRFTGSGSGTDFTLTISNVQSEDLA
DYFCQQYSNYPLTFGAGTKLELKGSHHHHHH"
misc_feature complement(3961..4017)
/note="sB1H"
misc_feature complement(4207..5144)
/note="intron"
misc_feature complement(5145..5347)
/note="5UTR"
regulatory complement(5348..5643)
/note="SV40 late promoter"
/regulatory_class="promoter"
regulatory 5399..5680
/note="SV40 early promoter"
/regulatory_class="promoter"
misc_feature 5751..5996
/note="SV40 large T-antigen"
CDS 5751..6275
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL88238.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
misc_feature 5997..6342
/note="intron"
misc_feature 6343..7405
/note="part of SV40 large t-antigen"
rep_origin complement(7689..8091)
/note="pMB1 ori"
CDS complement(8632..9492)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88239.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2824 a 2040 c 2207 g 2629 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
3061 tgtatcttat catgtctgga tccggctcta gcttggattc tcaccaataa aaaacgcccg
3121 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
3181 caacaggagt ccaagctcag ctaattaagc ttagtgatgg tgatggtgat gagatccttt
3241 cagctccagc ttggtcccag caccgaacgt gagaggatag ttgctatatt gttggcagaa
3301 ataatctgcc aggtcttcag actgcacatt gctgatggtg agagtgaaat ctgtcccaga
3361 tccactgcct gtgaagcgat cagggactcc agtgtaccga ctggatgtcg agtcaatcag
3421 tagtttagga gattgtccta gtttctgttg ataccaggct acatcagtac ccacattctg
3481 actggccttg caggtgatgc tgaccctgtc tcctactgat gtggacatga atttttgaga
3541 ctgggtcatc acaatgtccg atccgccacc gccagagcca cctccgcctg aaccgcctcc
3601 acctgaggag acagtgaccg tggtccctgc gccccagaca tcgaagtacc ccccccccca
3661 gttaggactt gcacagtaat acaaggccgt gtcctcagac ctcagactgc ccatttgcag
3721 gtacagggcg ttcctggcat tgtctctgga gatggtgaat cggcccttca cactgtctgg
3781 atagtaggtg taattaccac cacgactaat ggttgcgacc cactccagcc tcttctccgg
3841 agtctggcga acccaagaca tgtcataggt actgaaagcg aatccagagg ctgcacagga
3901 gagtttcagg gaccctccag gcttcactaa gcctccccca gactccacca gcttcacttc
3961 acactggaca ccttttaaaa ttaggacaag gaaaatcaag ctcagcccaa agttcatggt
4021 gaggggtccg tgttcagtgc tgatcactga atgtgaggaa ctgggaattc caatgcttcg
4081 tttcgtatca cacaccccaa agccttctgc tttgaatgct gcccttcttc agggcttaat
4141 ttctctagag gctgcagccg accccggatc cggagctttt agagcagaag taacacttcc
4201 gtacaggcct agaagtaaag gcaacatcca ctgaggagca gttctttgat ttgcaccacc
4261 aggagcctca aatttttcaa taaattcacc tgactgcaca ttaggacttt ggctttgagc
4321 ttcccacctc tcagttactt gctgaatact gtctgcttca tcaatattat cataggtgtg
4381 cccaaatgat atttgcaacc cttccctgtt ggctacttgt ctcaccattg taggcctaat
4441 gggagacaaa gtagagtagt aatcttgtaa agagttatac caattaacag gagcattaat
4501 tactgtccaa gtagtttcct ctaaaaacct tgccaaactg tcccttaaat atctttgggt
4561 tcttctttca agctcctgtg aggtgagcct aggaatgtca ttttgtatta cacgccaaaa
4621 agcttgagaa atggcattaa aaagtgttgg accccaatgt ctggggtcaa gatactgaac
4681 actgtgaaca aaggtttgta ctccaggaaa taaaatatca tagtaatcat ctggcctata
4741 caaatctaca gccattcctg gttgttgata taaaccaaca gtagaaactt tgtgatccca
4801 gtcactaaaa aatctatacc ccacttgagc aacagcgctc acaccagtca cagtttgcag
4861 taaagctgca aatccagcta tagcagcagg agccccagat atcacagcat aggcctgtgg
4921 agtgaggcct atagcagcaa ttgcctcaga ggttgttagg ccttcaacag tagcaacaga
4981 tgcaagttgc acttcaattg cagcagcggc ctctccagca gcaatttcag ctactgaaaa
5041 tccagtagca gcagcagctt cagacacagt agcaattagg tcccccaaca gtgttaaagc
5101 agcacccatg gacctgaaat aaaagacaaa aagactaaac ttaccagtta actttctggt
5161 ttttcagtta acctttctgg ttttttgcgt ttcccgtcaa cagtatcttc cccttcacaa
5221 aattgcagca aaagctctaa aacaaacaca aaaaggcgtt gagctgtttt tttactttca
5281 gtccatgacc tacgaacctt aacggaggcc tggcgtgaca gccggcgcag caccatggcc
5341 tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct
5401 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
5461 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
5521 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
5581 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
5641 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
5701 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttgcaaag atggataaag
5761 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtgcct
5821 gggggaatat tcctctgatg agaaaggcat atttaaaaaa atgcaaggag tttcatcctg
5881 ataaaggagg agatgaagaa aaaatgaaga aaatgaatac tctgtacaag aaaatggaag
5941 atggagtaaa atatgctcat caacctgact ttggaggctt ctgggatgca actgaggtat
6001 ttgcttcttc cttaaatcct ggtgttgatg caatgtactg caaacaatgg cctgagtgtg
6061 caaagaaaat gtctgctaac tgcatatgct tgctgtgctt actgaggatg aagcatgaaa
6121 atagaaaatt atacaggaaa gatccacttg tgtgggttga ttgctactgc ttcgattgct
6181 ttagaatgtg gtttggactt gatctttgtg aaggaacctt acttctgtgg tgtgacataa
6241 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
6301 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
6361 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
6421 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
6481 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
6541 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
6601 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
6661 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
6721 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
6781 gaatatttga tgtatagtgc cttgactaga gatccatttt ctgttattga ggaaagtttg
6841 ccaggtgggt taaaggagca tgattttaat ccagaagaag cagaggaaac taaacaagtg
6901 tcctggaagc ttgtaacaga gtatgcaatg gaaacaaaat gtgatgatgt gttgttattg
6961 cttgggatgt acttggaatt tcagtacagt tttgaaatgt gtttaaaatg tattaaaaaa
7021 gaacagccca gccactataa gtaccatgaa aagcattatg caaatgctgc tatatttgct
7081 gacagcaaaa accaaaaaac catatgccaa caggctgttg atactgtttt agctaaaaag
7141 cgggttgata gcctacaatt aactagagaa caaatgttaa caaacagatt taatgatctt
7201 ttggatagga tggatataat gtttggttct acaggctctg ctgacataga agaatggatg
7261 gctggagttg cttggctaca ctgtttgttg cccaaaatgg attcagtggt gtatgacttt
7321 ttaaaatgca tggtgtacaa cattcctaaa aaaagatact ggctgtttaa aggaccaatt
7381 gatagtggta aaactacatt agcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
7441 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
7501 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
7561 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
7621 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
7681 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
7741 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
7801 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
7861 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
7921 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
7981 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
8041 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
8101 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
8161 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
8221 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
8281 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
8341 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
8401 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
8461 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
8521 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
8581 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
8641 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
8701 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
8761 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
8821 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
8881 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
8941 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
9001 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
9061 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
9121 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
9181 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
9241 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
9301 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
9361 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
9421 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
9481 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
9541 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
9601 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
9661 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
//