LOCUS LT727350 5800 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGSE6L, complete sequence.
ACCESSION LT727350
VERSION LT727350.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGSE6L
ORGANISM Mammalian expression vector pCAGGSE6L
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5800)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5800
/organism="Mammalian expression vector pCAGGSE6L"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3547."
/db_xref="taxon:1944759"
misc_feature 10..61
/note="rGlBf"
misc_feature 160..178
/note="5' UTR of mIghg2b"
CDS 179..883
/transl_table=11
/note="mIghg2b"
/note="light chain of mouse anti-hPLAP monoclonal antibody
(E6L)"
/protein_id="SJL88183.1"
/translation="MSVPTQVLGLLLLWLTDARCDIQMTQSPASLSVSVGESVTITCR
ASENIYSNLAWYQQKQGKSPQLLVYVATKLVDGVPSRFSGSGSGTQYSLKINSLQSED
FGSYYCQHFWDTPFTFGSGTKLEMKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFY
PKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATH
KTSTSPIVKSFNRNEC"
misc_feature 884..1052
/note="3' UTR of mIghg2b"
misc_feature 1061..1181
/note="rGlBf"
misc_feature 1182..1538
/note="3FR"
regulatory complement(1616..1698)
/note="lac"
/regulatory_class="promoter"
rep_origin 1840..1915
/note="SV40 ori"
regulatory 1946..2020
/note="polyA"
/regulatory_class="terminator"
regulatory complement(1946..2020)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(2135..2536)
/note="pMB1 ori"
CDS complement(3077..3937)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88184.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 4073..4450
/note="hCMV"
regulatory 4456..5797
/note="AG"
/regulatory_class="promoter"
misc_feature 4825..5749
/note="intron"
BASE COUNT 1266 a 1550 c 1564 g 1420 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 gctagagaaa ttaagccctg aagaagggca gcattcaaag cagaaggctt tggggtgtgt
121 gatacgaaac gaagcattgg aattccggat ccggccaagc ttgggatcac acacagacat
181 gagtgtgccc actcaggtcc tggggttgct gctgctgtgg cttacagatg ccagatgtga
241 catccagatg actcagtctc cagcctccct atccgtttct gtgggagaat ctgtcaccat
301 cacatgtcga gcaagtgaga atatttacag taatttagca tggtatcagc agaaacaggg
361 aaaatctcct cagctccttg tctatgttgc gacaaagtta gtagatggtg tgccatcaag
421 gttcagtggc agtggatcag gcacacagta ttccctcaag atcaacagcc tgcagtctga
481 agattttggg agttattact gtcaacattt ttgggacact ccattcacgt tcggctcggg
541 gacaaagttg gaaatgaaac gggctgatgc tgcaccaact gtatccatct tcccaccatc
601 cagtgagcag ttaacatctg gaggtgcctc agtcgtgtgc ttcttgaaca acttctaccc
661 caaagacatc aatgtcaagt ggaagattga tgggagtgaa cgacaaaatg gcgtcctgaa
721 cagttggact gatcaggaca gcaaagacag cacctacagc atgagcagca ccctcacgtt
781 gaccaaggac gagtatgaac gacataacag ctatacctgt gaggccactc acaagacatc
841 aacttcaccc attgtcaaga gcttcaacag gaatgagtgt tagagacaaa ggtcctgaga
901 cgccaccacc agctccccag ctccatccta tcttcccttc taaggtcttg gaggcttccc
961 cacaagcgac ctaccactgt tgcggtgctc caaacctcct ccccacctcc ttctcctcct
1021 cctccctttc cttccccccc cccccccccg tcgactctag ccaatgccct ggctcacaaa
1081 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
1141 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
1201 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
1261 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
1321 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
1381 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
1441 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
1501 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
1561 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
1621 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc
1681 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
1741 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
1801 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
1861 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
1921 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
1981 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
2041 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
2101 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
2161 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
2221 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
2281 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
2341 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
2401 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
2461 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
2521 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
2581 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
2641 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
2701 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
2761 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
2821 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
2881 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
2941 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
3001 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
3061 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
3121 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
3181 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
3241 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
3301 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
3361 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
3421 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
3481 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
3541 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
3601 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
3661 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
3721 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
3781 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
3841 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
3901 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
3961 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
4021 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
4081 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
4141 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
4201 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
4261 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
4321 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
4381 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
4441 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
4501 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
4561 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg
4621 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
4681 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt
4741 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
4801 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
4861 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
4921 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
4981 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
5041 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
5101 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
5161 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
5221 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
5281 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
5341 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg
5401 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
5461 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
5521 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
5581 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
5641 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
5701 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
5761 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
//