LOCUS LT727347 6997 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pLT10lacZT3, complete sequence.
ACCESSION LT727347
VERSION LT727347.1
KEYWORDS .
SOURCE Vector pLT10lacZT3
ORGANISM Vector pLT10lacZT3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6997)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6997
/organism="Vector pLT10lacZT3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3520."
/db_xref="taxon:1945327"
regulatory 24..40
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 41..103
/note="5' UTR of T7 gene 10, incl. RBS"
CDS 104..3160
/transl_table=11
/note="lacZ"
/protein_id="SJL88175.1"
/translation="MDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ
QLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPI
TVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDS
RLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQIS
DFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDE
RGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPL
WYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVII
WSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFP
AVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWV
DQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQF
RLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQ
PESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEM
DFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDP
NAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGS
GQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWD
LPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLH
AEEGTWLNIDGFHMGIGGDDSWSPSVSAXXQLSAGRYHYQLVWCQK"
regulatory complement(3592..3608)
/note="T3 promoter"
/regulatory_class="promoter"
regulatory 3626..3673
/note="T7 terminator"
/regulatory_class="terminator"
regulatory 3747..3794
/note="T7 terminator"
/regulatory_class="terminator"
regulatory 3863..3944
/note="fd terminator"
/regulatory_class="terminator"
regulatory 3973..4054
/note="fd terminator"
/regulatory_class="terminator"
rep_origin 4152..4607
/note="f1 ori"
rep_origin complement(4611..4984)
/note="pMB1 ori"
CDS complement(5525..6385)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88176.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 6751..6881
/note="PL promoter"
/regulatory_class="promoter"
BASE COUNT 1678 a 1784 c 1787 g 1742 t
ORIGIN
1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc
61 ctctagaaat aattttgttt aactttaaga aggagatata catatggatc ccgtcgtttt
121 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
181 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
241 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga
301 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca
361 gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc
421 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga
481 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca
541 tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc cgtctgaatt
601 tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgttg
661 gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga
721 cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt
781 taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg
841 tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg ccagcggcac
901 cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacact
961 acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc
1021 ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg
1081 tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat
1141 tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac
1201 gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca
1261 ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga
1321 tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg
1381 ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc
1441 gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct
1501 gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc
1561 cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc
1621 cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg
1681 cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gtttcgctaa
1741 atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt
1801 ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga
1861 ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg
1921 cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc agttccgttt
1981 atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct
2041 cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga
2101 tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc
2161 cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc
2221 cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc
2281 cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct
2341 gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg
2401 cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa
2461 cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa
2521 ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc
2581 tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat
2641 cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga
2701 agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc tggcgcaggt
2761 agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac
2821 tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt
2881 cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg
2941 gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag
3001 ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat
3061 ggggattggt ggcgacgact cctggagccc gtcagtatcg gcgnnnnnnc agctgagcgc
3121 cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg
3181 tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg
3241 gatgttcggt ttattctttt tcttttactt ttttatcatg ggagcctact tcccgttttt
3301 cccgatttgg ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc
3361 cgctatttct ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact
3421 cgggctgcgc aaatacctgc tgtggattat taccggcatg ttagtgatgt ttgcgccgtt
3481 ctttattttt atcttcgggc cactgttaca atacaacatt ttagtaggat cgattgttgg
3541 tggtatttat ctaggctttt gttttaacgc cggggccgac ttggccttcc ctttagtgag
3601 ggttaataaa cttggtgagc aataactagc ataacccctt ggggcctcta aacgggtctt
3661 gaggggtttt ttgctgaaag gaggaactat atgcgctcat acgatatgaa cgttgagact
3721 gccgctgagt tatcagtgag caataactag cataacccct tggggcctct aaacgggtct
3781 tgaggggttt tttgctgaaa ggaggaacta tatccggata gcttatcgct agaggtcgaa
3841 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
3901 tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttccaagcta attcacctcg
3961 aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt tttttggaga
4021 ttttcaacgt gaaaaaatta ttattcgcaa ttccaagctc tgcctcgcgc gtttcggtga
4081 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
4141 ggatgcagat cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
4201 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
4261 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
4321 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
4381 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
4441 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
4501 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
4561 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttgat ctgcgctcgg
4621 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
4681 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
4741 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
4801 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
4861 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
4921 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
4981 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
5041 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
5101 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
5161 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
5221 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
5281 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
5341 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
5401 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
5461 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
5521 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
5581 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
5641 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
5701 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
5761 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
5821 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
5881 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
5941 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
6001 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
6061 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
6121 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
6181 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
6241 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
6301 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
6361 cgacacggaa atgttgaata ctcatactct tcctttttca atattatgta agcagacagt
6421 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
6481 acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat
6541 cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc
6601 acctacaaca aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg
6661 attcaggcct ggtatgagtc agcaacacct tcttcacgag gcagacctca gcgccggtga
6721 tgccggccac gatgcgtccg gcgtagagga tctctcacct accaaacaat gcccccctgc
6781 aaaaaataaa ttcatataaa aaacatacag ataaccatct gcggtgataa attatctctg
6841 gcggtgttga cataaatacc actggcggtg atactgagca catcagcagg acgcactgac
6901 caccatgaag gtgacgctct taaaattaag ccctgaagaa gggcagcatt caaagcagaa
6961 ggctttgggg tgtgtgatac gaaacgaagc attggaa
//