LOCUS LT727346 5023 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pSV/TXBP15-1-AS, complete sequence.
ACCESSION LT727346
VERSION LT727346.1
KEYWORDS .
SOURCE Vector pSV/TXBP15-1-AS
ORGANISM Vector pSV/TXBP15-1-AS
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5023)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5023
/organism="Vector pSV/TXBP15-1-AS"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3515."
/db_xref="taxon:1945409"
regulatory 45..326
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 283..358
/note="SV40 ori"
CDS 413..>632
/transl_table=11
/note="GALbd"
/protein_id="SJL88172.1"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
KRSPLTRAHLTEVESRLERLEQLFLLIFP"
CDS complement(690..2381)
/transl_table=11
/note="TAXBP"
/protein_id="SJL88173.1"
/translation="MERELNHEKERCDQLQAEQKGLTEVTQSLKMENEEFKKRFSDAT
SKAHQLEEDIVSVTHKAIEKETELDSLKDKLKKAQHEREQLECQLKTEKDEKELYKVH
LKNTEIENTKLMSEVQTLKNIDGNKESVITHFKEEIGRLQLCLAEKENLQRTFLLTTS
SKEDTCFLKEQLRKAEEQVQATRQEVVFLAKELSDAVNVRDRTMADLHTARLENEKVK
KQLADAVAELKLNAMKKDQDKTDTLEHELRREVEDLKLRLQMAADHYKEKFKECQRLQ
KQINKLSDQSANNNNVFTKKTGNQQKVNDASVNTDPATSASTVDVKPSPSAAEADFDI
VTKGQVCEMTKEIADKTEKYNKCKQLLQDEKAKCNKYADELAKMELKWKEQVKIAENV
KLELAEVQDNYKELKRSLENPAERKMEDGADGAFYPDEIQRPPVRVPSWGLEDNVVCS
QPARNFSRPDGLEDSEDSKEDENVPTAPDPPSQHLRGHGTGFCFDSSFDVHKKCPLCE
LMFPPNYDQSKFEEHVESHWKVCPMCSEQFPPDYDQQVFERHVQTHFDQNVLNFD"
regulatory 2530..2626
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2530..2626)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(2789..3191)
/note="pMB1 ori"
CDS complement(3732..4592)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88174.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERHTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1248 a 1205 c 1065 g 1505 t
ORIGIN
1 tatgtatcat acacatacga tttaggtgac actatagaac tcgactgtgg aatgtgtgtc
61 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
121 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
181 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
241 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
301 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
361 ttggaggcct aggcttttgc aaaaagctag atctaagcta gcttcctgaa agatgaagct
421 actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca agtgctccaa
481 agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct actctcccaa
541 aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa ggctagaaag
601 actggaacag ctatttctac tgatttttcc tcgagttttt tttttttttt ttttactgct
661 aaactatatt aactcataat aaaaagtaac tagtcaaaat ttagaacatt ctgatcaaaa
721 tgggtctgca catgcctttc aaacacctgc tggtcatagt caggagggaa ctgctcgctg
781 cacatcgggc acaccttcca gtgactttca acatgttctt caaatttgct ctgatcatag
841 ttaggaggaa acattaactc acagagggga cacttcttgt gaacatcaaa gctggaatca
901 aagcaaaagc ctgtcccatg cccacgtaaa tgttgacttg gaggatcagg agcagtaggc
961 acattctcat cttctttgct atcctcagag tcctctaagc catcaggccg actaaagttt
1021 cgagcaggct ggctgcagac aacattgtct tccagtcccc aagaggggac tctgacaggt
1081 ggcctttgta tttcatctgg gtaaaaagca ccatctgctc catcttccat tttcctttct
1141 gctggatttt ctagactcct tttaagttct ttataattgt cctgtacttc agctagttca
1201 agttttacat tttcagcaat tttcacttgt tctttccatt tcagctccat ttttgcaagt
1261 tcatcagcat atttattgca ttttgctttc tcatcctgca agagttgttt acatttatta
1321 tacttttctg ttttgtcagc aatttctttg gtcatttcac agacttgccc ctttgttact
1381 atgtcaaaat ctgcctctgc tgcagaaggt gatggcttta catctacagt agaggcagaa
1441 gtggctgggt ctgtgtttac tgaagcatca ttcactttct gctgattccc cgttttcttt
1501 gtgaagacat tattattatt agctgattga tctgaaagtt tgtttatttg tttttggagc
1561 ctttggcatt ccttaaattt ttctttataa tggtctgcag ccatctgaag acggagtttc
1621 agatcttcaa cttctcttct tagttcgtgt tccagtgtat cagtcttgtc ctgatctttt
1681 ttcatagcat ttagtttaag ttctgccact gcatcagcta actgcttttt cactttctcg
1741 ttttccaagc gtgcagtatg caggtctgcc atcgttctgt ctcgtacgtt gacagcatca
1801 ctgagttctt tagccagaaa gacaacttct tgccgagttg cctgaacctg ttcctctgct
1861 ttacgaagtt gctcctttaa aaaacaagta tcttctttac ttgaggttgt aagcaggaaa
1921 gttctttgca gattttcctt ttcagccaaa cataactgca gcctgccaat ctcttctttg
1981 aaatgagtaa tcacgctttc tttgttccca tctatatttt ttaaagtctg gacctctgac
2041 ataagcttgg tattttctat ttctgtattc ttcaaatgta ccttataaag ttccttttca
2101 tccttctctg tcttcaactg acattcaagt tgttctcttt catgttgtgc cttcttgagt
2161 ttgtccttta aactgtctaa ttcggtttct ttttcaattg ctttatgtgt tactgacaca
2221 atatcttcct caagctgatg ggctttggat gtagcatcac tgaacctctt cttaaactct
2281 tcattttcca tttttaagct ttgtgttact tcagtaagac ccttttgttc tgcttgcagt
2341 tggtcacatc tttctttctc atggttaagt tctctttcca ttctccctcg tgccgaattc
2401 ccggggatcc gtcgacgcgt ctgcagaagc ttctagataa gtaatgatca taatcagcca
2461 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
2521 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
2581 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
2641 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgcc ggtctcccta
2701 tagtgagtcg tattaatttc gataagccag gttaacctgc attaatgaat cggccaacgc
2761 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
2821 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
2881 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
2941 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
3001 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
3061 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
3121 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
3181 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
3241 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
3301 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
3361 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
3421 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
3481 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
3541 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
3601 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
3661 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
3721 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
3781 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
3841 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
3901 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
3961 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
4021 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtgacgctc gtcgtttggt
4081 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
4141 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
4201 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
4261 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
4321 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
4381 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
4441 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
4501 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
4561 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
4621 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
4681 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
4741 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
4801 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
4861 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
4921 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
4981 gacatattgt cgttagaacg cggctacaat taatacataa cct
//