LOCUS LT727342 3851 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial expression vector pISA417, complete sequence.
ACCESSION LT727342
VERSION LT727342.1
KEYWORDS .
SOURCE Bacterial expression vector pISA417
ORGANISM Bacterial expression vector pISA417
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3851)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..3851
/organism="Bacterial expression vector pISA417"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3477."
/db_xref="taxon:1944672"
misc_feature complement(399..775)
/note="3' UTR of the cobA gene"
CDS complement(776..1549)
/transl_table=11
/note="cobA"
/protein_id="SJL88161.1"
/translation="MTTTLLPGTVTLVGAGPGDPELVTVAGLRAVQQAEVILYDRLAP
QDLLSEASDDAELVPVGKIPRGHYVPQEEINQLLVAHAREGRKVVRLKGGDSFVFGRG
GEEWQACAEAGIPVRVIPGVSSATAGPALAGIPLTHRHLVQGFTVVSGHVSPSDERSE
VPWRQLAKDRLTLVILMGVAHMRDIAPELMAGGLPADTPVRVVSNASLASQESWRTTL
GDAVADMDAHHVRPPALVVVGTLAGVDLSHPDHRAPSDH"
misc_feature complement(1550..1584)
/note="5' UTR of the cobA gene"
regulatory complement(1673..1756)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(1848..2250)
/note="pMB1 ori"
CDS complement(2791..3651)
/transl_table=11
/note="ampicillin"
/protein_id="SJL88162.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 826 a 1083 c 1121 g 817 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgacgcgcgt ggcatccgat
421 cggcgtgact ccccgcgcac cgtgaagccg acctcgcggc ccagggcggt gccggacagt
481 tcggcgagtc ggtgggccgc cgcgcgggcg gcgatgcgcc ggggttgggt gaccacgacg
541 cgcccggcat ggtgatgcgc ggccaggggc ggcatcacgg tggtcttgcc cgagcccgga
601 ggggcctgca ccaccaggcg cgcatccggc gccgtgaggg ccgcggcgag ctcgtccagg
661 cgcccaccaa ccggcagccc ggcggctatc cgatcgaggt caaacacgtt cggcagtgtc
721 tcacggccgc aggccacgcc gatcgatcag caggtgcccg gcggccggcc gggactcagt
781 ggtcgctggg cgcgcgatgg tcgggatgcg acaggtcgac gccggccagg gtacccacca
841 ccaccagcgc gggcggacgc acgtggtgcg cgtccatgtc ggccacggca tcgcccagcg
901 tggtgcgcca cgattcctgg ctggccaggc tcgcattgct caccacgcgc acgggggtgt
961 cggcaggcag cccgccggcc atcaattccg gcgcgatgtc gcgcatatgg gccacgccca
1021 tcaggatcac cagcgtgagc cggtccttgg cgagttggcg ccatggcacc tcggagcgct
1081 cgtcgctggg cgatacatgc cccgacacga cggtgaaccc ctgcaccagg tggcgatggg
1141 tcagcgggat gccggccagc gccgggcccg cggtggccga ggagactccc gggatcacgc
1201 gcaccgggat gccggcctcg gcgcaggcct gccattcctc gccgccacgc ccgaagacga
1261 acgagtcgcc acccttgagg cgcaccacct tgcggccctc gcgggcgtgc gcgacgagca
1321 gttggttgat ctcctcctgg ggcacatagt ggccgcgcgg gatcttgccg accggcacga
1381 gttcggcgtc gtcggacgcc tccgacagca ggtcctgcgg ggcgagccgg tcgtagagga
1441 tcacctcggc ctgctgcacg gcccgcaggc cggccacggt gacgagttca gggtcgccgg
1501 gcccggcgcc gacgagggtg acagtgccgg gcaacagtgt ggtggtcatc gggtttcctc
1561 atcggtgttg gtggtgtcgt cgnnnnctct agagtcgacc tgcaggcatg caagcttggc
1621 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
1681 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
1741 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
1801 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
1861 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
1921 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
1981 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
2041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
2101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
2161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
2221 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
2281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
2341 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
2401 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
2461 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
2521 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
2581 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
2641 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
2701 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
2761 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
2821 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
2881 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
2941 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
3001 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
3061 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
3121 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
3181 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
3241 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
3301 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
3361 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
3421 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
3481 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
3541 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
3601 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
3661 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
3721 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
3781 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
3841 gccctttcgt c
//