LOCUS LT727341 5453 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pIRES-LUC, complete sequence.
ACCESSION LT727341
VERSION LT727341.1
KEYWORDS .
SOURCE Vector pIRES-LUC
ORGANISM Vector pIRES-LUC
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5453)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5453
/organism="Vector pIRES-LUC"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3472."
/db_xref="taxon:1945321"
rep_origin 4..459
/note="f1 ori"
regulatory 626..642
/note="T7 promoter"
/regulatory_class="promoter"
regulatory complement(759..855)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 759..855
/note="SV40 polyA early"
/regulatory_class="terminator"
CDS complement(943..2595)
/transl_table=11
/note="LUCm"
/protein_id="SJL88159.1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
misc_feature complement(2609..3196)
/note="5' UTR of the EMCV polyprotein gene (IRES)"
regulatory complement(3267..3283)
/note="T3 promoter"
/regulatory_class="promoter"
regulatory complement(3347..3430)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(3522..3924)
/note="pMB1 ori"
CDS complement(4465..5325)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88160.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1343 a 1373 c 1304 g 1433 t
ORIGIN
1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
661 gccccccctc gacggatcct tatcgatttt accacatttg tagaggtttt acttgcttta
721 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
781 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
841 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
901 tatcatgtct gctcgaagcg gccggccgcc ccgactctag aattacacgg cgatctttcc
961 gcccttcttg gcctttatga ggatctctct gatttttctt gcgtcgagtt ttccggtaag
1021 acctttcggt acttcgtcca caaacacaac tcctccgcgc aactttttcg cggttgttac
1081 ttgactggcg acgtaatcca cgatctcttt ttccgtcatc gtctttccgt gctccaaaac
1141 aacaacggcg gcgggaagtt caccggcgtc atcgtcggga agacctgcga cacctgcgtc
1201 gaagatgttg gggtgttgga gcaagatgga ttccaattca gcgggagcca cctgatagcc
1261 tttgtactta atcagagact tcaggcggtc aacgatgaag aagtgttcgt cttcgtccca
1321 gtaagctatg tctccagaat gtagccatcc atccttgtca atcaaggcgt tggtcgcttc
1381 cggattgttt acataaccgg acataatcat aggacctctc acacacagtt cgcctctttg
1441 attaacgccc agcgttttcc cggtatccag atccacaacc ttcgcttcaa aaaatggaac
1501 aactttaccg accgcgcccg gtttatcatc cccctcgggt gtaatcagaa tagctgatgt
1561 agtctcagtg agcccatatc cttgcctgat acctggcaga tggaacctct tggcaaccgc
1621 ttccccgact tccttagaga ggggagcgcc accagaagca atttcgtgta aattagataa
1681 atcgtatttg tcaatcagag tgcttttggc gaagaaggag aatagggttg gcaccagcag
1741 cgcactttga atcttgtaat cctgaaggct cctcagaaac agctcttctt caaatctata
1801 cattaagacg actcgaaatc cacatatcaa atatccgagt gtagtaaaca ttccaaaacc
1861 gtgatggaat ggaacaacac ttaaaatcgc agtatccgga atgatttgat tgccaaaaat
1921 aggatctctg gcatgcgaga atctcacgca ggcagttcta tgaggcagag cgacaccttt
1981 aggcagacca gtagatccag aggagttcat gatcagtgca attgtcttgt ccctatcgaa
2041 ggactctggc acaaaatcgt attcattaaa accgggaggt agatgagatg tgacgaacgt
2101 gtacatcgac tgaaatccct ggtaatccgt tttagaatcc atgataataa ttttttggat
2161 gattgggagc tttttttgca cgttcaaaat tttttgcaac ccctttttgg aaacgaacac
2221 cacggtaggc tgcgaaatgc ccatactgtt gagcaattca cgttcattat aaatgtcgtt
2281 cgcgggcgca actgcaactc cgataaataa cgcgcccaac accggcataa agaattgaag
2341 agagttttca ctgcatacga cgattctgtg atttgtattc agcccatatc gtttcatagc
2401 ttctgccaac cgaacggaca tttcgaagta ctcagcgtaa gtgatgtcca cctcgatatg
2461 tgcatctgta aaagcaattg ttccaggaac cagggcgtat ctcttcatag ccttatgcag
2521 ttgctctcca gcggttccat cttccagcgg atagaatggc gccgggcctt tctttatgtt
2581 tttggcgtct tccatggttg tggcaagctt atcatcgtgt ttttcaaagg aaaaccacgt
2641 ccccgtggtt cggggggcct agacgttttt taacctcgac taaacacatg taaagcatgt
2701 gcaccgaggc cccagatcag atcccataca atggggtacc ttctgggcat ccttcagccc
2761 cttgttgaat acgcttgagg agagccattt gactctttcc acaactatcc aactcacaac
2821 gtggcactgg ggttgtgccg cctttgcagg tgtatcttat acacgtggct tttggccgca
2881 gaggcacctg tcgccaggtg gggggttccg ctgcctgcaa agggtcgcta cagacgttgt
2941 ttgtcttcaa gaagcttcca gaggaactgc ttccttcacg acattcaaca gaccttgcat
3001 tcctttggcg agaggggaaa gacccctagg aatgctcgtc aagaagacag ggccaggttt
3061 ccgggccctc acattgccaa aagacggcaa tatggtggaa aataacatat agacaaacgc
3121 acaccggcct tattccaagc ggcttcggcc agtaacgtta gggggggggg agggagaggg
3181 gggggggggg ggggggcgga attctctaga ggatccacta gttctagagc ggccgccacc
3241 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
3301 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
3361 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
3421 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
3481 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
3541 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
3601 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
3661 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
3721 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
3781 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
3841 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
3901 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
3961 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
4021 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
4081 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
4141 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
4201 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
4261 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
4321 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
4381 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
4441 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
4501 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
4561 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
4621 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
4681 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
4741 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
4801 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
4861 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
4921 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
4981 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
5041 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
5101 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
5161 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
5221 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
5281 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
5341 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
5401 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//