LOCUS LT727340 6345 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGShMnSOD, complete sequence.
ACCESSION LT727340
VERSION LT727340.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGShMnSOD
ORGANISM Mammalian expression vector pCAGGShMnSOD
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6345)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6345
/organism="Mammalian expression vector pCAGGShMnSOD"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3451."
/db_xref="taxon:1944872"
misc_feature 6..39
/note="5UTR"
CDS 40..708
/transl_table=11
/note="hMnSOD"
/protein_id="SJL88157.1"
/translation="MLSRAVCGTSRQLAPALGYLGSRQKHSLPDLPYDYGALEPHINA
QIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGDVTAQIALQPALKFNGGGHINHSIFW
TNLSPNGGGEPKGELLEAIKRDFGSFDKFKEKLTAASVGVQGSGWGWLGFNKQRGHLQ
IAACPNQDPLQGTTGLIPLLGIDVWEHAYYLQYKNVRPDYLKAIWNVINWENVTERYM
ACKK"
misc_feature 709..801
/note="3UTR"
regulatory 887..978
/note="polyA"
/regulatory_class="terminator"
misc_feature 979..1335
/note="3FR"
regulatory 1359..1433
/note="polyA"
/regulatory_class="terminator"
regulatory complement(1359..1433)
/note="polyA"
/regulatory_class="terminator"
misc_feature 1489..2234
/note="PY"
rep_origin 2122..2234
/note="PY-O"
rep_origin 2385..2460
/note="SV40 ori"
regulatory 2491..2565
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2491..2565)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(2680..3081)
/note="pMB1 ori"
CDS complement(3622..4482)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88158.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 4618..4995
/note="hCMV"
regulatory 5001..6342
/note="AG"
/regulatory_class="promoter"
misc_feature 5370..6294
/note="intron"
BASE COUNT 1425 a 1651 c 1706 g 1563 t
ORIGIN
1 ttcggcggcg gcatcagcgg tagcaccagc actagcagca tgttgagccg ggcagtgtgc
61 ggcaccagca ggcagctggc tccggctttg gggtatctgg gctccaggca gaagcacagc
121 ctccccgacc tgccctacga ctacggcgcc ctggaacctc acatcaacgc gcagatcatg
181 cagctgcacc acagcaagca ccacgcggcc tacgtgaaca acctgaacgt caccgaggag
241 aagtaccagg aggcgttggc caagggagat gttacagccc agatagctct tcagcctgca
301 ctgaagttca atggtggtgg tcatatcaat catagcattt tctggacaaa cctcagccct
361 aacggtggtg gagaacccaa aggggagttg ctggaagcca tcaaacgtga ctttggttcc
421 tttgacaagt ttaaggagaa gctgacggct gcatctgttg gtgtccaagg ctcaggttgg
481 ggttggcttg gtttcaataa gcaacgggga cacttacaaa ttgctgcttg tccaaatcag
541 gatccactgc aaggaacaac aggccttatt ccactgctgg ggattgatgt gtgggagcac
601 gcttactacc ttcagtataa aaatgtcagg cctgattatc taaaagctat ttggaatgta
661 atcaactggg agaatgtaac tgaaagatac atggcttgca aaaagtaaac cacgatcgtt
721 atgctgatca taccctaatg atcccagcaa gataatgtcc tgtcttctaa gatgtgcatc
781 aagcctggta catactgaaa cccccgaatt cactcctcag gtgcaggctg cctatcagaa
841 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
901 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
961 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
1021 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
1081 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
1141 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
1201 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
1261 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
1321 tctcttatgg agatccctcg acctgcagcc caagcttgaa cttgtttatt gcagcttata
1381 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttatcactgc
1441 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcccgccat
1501 ggtatcaacg ccatatttct atttacagta gggacctctt cgttgtgtag gtaccgctgt
1561 attcctaggg aaatagtaga ggcaccttga actgtctgca tcagccatat agcccccgct
1621 gttcgactta caaacacagg cacagtactg acaaacccat acacctcctc tgaaataccc
1681 atagttgcta gggctgtctc cgaactcatt acaccctacc aagtgagagc tgtaatttcg
1741 cgatcaaggg cagcgagggc ttctccagat aaaatagctt ctgccgagag tcccgtaagg
1801 gtagacactt cagctaatcc ctcgatgagg tctactagaa tagtcagtgc ggctcccatt
1861 ttgaaaattc acttacttga tcagcttcag aagatgggcg agggcctcca acacagtaat
1921 tttcctcccg actcttaaaa tagaaaatgt caagtcagtt aaggaggaag tggactaact
1981 gacgcagctg gccgtgcgac atcctctttt aattagttgc taggcaacgc cctccagagg
2041 gcgtgtggtt ttgcaagagg aagcaaaagc ctctccaccc aggcctagaa tgtttccacc
2101 caatcattac tatgacaaca gctgtttttt ttagtattaa gcagaggccg gggacccctg
2161 ggcccgctta ctctggagaa aaagaagaga ggcattgtag aggcttccag aggcaacttg
2221 tcaaaacagg actgcttcta tttctgtcac actgtctggc cctgtcacaa ggtccagcac
2281 ctccataccc cctttaataa gcagtttggg aacgggtgcg ggtcttactc cgcccatccc
2341 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
2401 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
2461 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
2521 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
2581 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
2641 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
2701 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
2761 taatacggtt atccacagaa tcagggataa cgcaggaaag aacatgtgag caaaaggcca
2821 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
2881 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
2941 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
3001 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
3061 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
3121 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
3181 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
3241 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
3301 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
3361 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
3421 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
3481 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
3541 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
3601 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
3661 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
3721 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
3781 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
3841 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
3901 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
3961 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
4021 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
4081 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
4141 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
4201 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
4261 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
4321 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
4381 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
4441 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
4501 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
4561 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac
4621 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
4681 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
4741 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
4801 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
4861 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
4921 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
4981 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
5041 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
5101 gggcgggggg gggggggggg ccccccccag gcggggcggg gcggggcgag gggcggggcg
5161 gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
5221 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
5281 tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
5341 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
5401 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
5461 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
5521 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
5581 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
5641 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
5701 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
5761 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
5821 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
5881 cgcctcgggc cggggagggc tcgggggaag gggcgcggcg gcccccggag cgccggcggc
5941 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
6001 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
6061 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
6121 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
6181 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
6241 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
6301 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaa
//