LOCUS LT727339 6479 bp DNA circular SYN 06-FEB-2017
DEFINITION Reporter vector pBIC, complete sequence.
ACCESSION LT727339
VERSION LT727339.1
KEYWORDS .
SOURCE Reporter vector pBIC
ORGANISM Reporter vector pBIC
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6479)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6479
/organism="Reporter vector pBIC"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3450."
/db_xref="taxon:1945198"
regulatory complement(64..160)
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory 64..160
/note="SV40 polyA late"
/regulatory_class="terminator"
misc_feature complement(161..779)
/note="3' UTR of SV40 small t-antigen"
misc_feature complement(867..911)
/note="3' UTR of LUC"
CDS complement(912..2564)
/transl_table=11
/note="LUC"
/protein_id="SJL88154.1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
misc_feature complement(2600..3060)
/note="5' UTR of FMDV-POLYP (IRES)"
CDS complement(3126..3785)
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL88155.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
regulatory complement(3813..3921)
/note="HSV-TK promoter"
/regulatory_class="promoter"
regulatory complement(3954..3970)
/note="T7 promoter"
/regulatory_class="promoter"
rep_origin complement(4038..4440)
/note="pMB1 ori"
CDS complement(4981..5841)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88156.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1700 a 1553 c 1494 g 1732 t
ORIGIN
1 ggggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
61 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
121 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
181 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
241 gatctctagt caaggcacta tacatcaaat attccttatt aaccccttta caaattaaaa
301 agctaaaggt acacaatttt tgagcatagt tattaatagc agacactcta tgcctgtgtg
361 gagtaagaaa aaacagtatg ttatgattat aactgttatg cctacttata aaggttacag
421 aatatttttc cataattttc ttgtatagca gtgcagcttt ttcctttgtg gtgtaaatag
481 caaagcaagc aagagttcta ttactaaaca cagcatgact caaaaaactt agcaattctg
541 aaggaaagtc cttggggtct tctacctttc tcttcttttt tggaggagta gaatgttgag
601 agtcagcagt agcctcatca tcactagatg gcatttcttc tgagcaaaac aggttttcct
661 cattaaaggc attccaccac tgctcccatt catcagttcc ataggttgga atctaaaata
721 cacaaacaat tagaatcagt agtttaacac attatacact taaaaatttt atatttacct
781 tagagcttta aatctctgta ggtagtttgt ccaattatgt cacaccacag aagtaaggtt
841 ccttcacaaa gagatcctct agagtcatta caatagctaa gaatttcgtc atcgctgaat
901 acagttacat tttacaattt ggactttccg cccttcttgg cctttatgag gatctctctg
961 atttttcttg cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact
1021 cctccgcgca actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt
1081 tccgtcatcg tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca
1141 tcgtcgggaa gacctgccac gcccgcgtcg aagatgttgg ggtgttgtaa caatatcgat
1201 tccaattcag cgggggccac ctgatatcct ttgtatttaa ttaaagactt caagcggtca
1261 actatgaaga agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca
1321 tccttgtcaa tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata
1381 ggtcctctga cacataattc gcctctctga ttaacgccca gcgttttccc ggtatccaga
1441 tccacaacct tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc
1501 ccctcgggtg taatcagaat agctgatgta gtctcagtga gcccatatcc ttgtcgtatc
1561 cctggaagat ggaagcgttt tgcaaccgct tccccgactt ctttcgaaag aggtgcgccc
1621 ccagaagcaa tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg
1681 aagaatgaaa atagggttgg tactagcaac gcactttgaa ttttgtaatc ctgaagggat
1741 cgtaaaaaca gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa
1801 tatccgagtg tagtaaacat tccaaaaccg tgatggaatg gaacaacact taaaatcgca
1861 gtatccggaa tgatttgatt gccaaaaata ggatctctgg catgcgagaa tctgacgcag
1921 gcagttctat gcggaagggc cacaccctta ggtaacccag tagatccaga ggaattcatt
1981 atcagtgcaa ttgttttgtc acgatcaaag gactctggta caaaatcgta ttcattaaaa
2041 ccgggaggta gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt
2101 ttagaatcca tgataataat tttctggatt attggtaatt ttttttgcac gttcaaaatt
2161 ttttgcaacc cctttttgga aacaaacact acggtaggct gcgaaatgtt catactgttg
2221 agcaattcac gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac
2281 gcgcccaaca ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga
2341 tttgtattca gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtat
2401 tccgcgtacg tgatgttcac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc
2461 agggcgtatc tcttcatagc cttatgcagt tgctctccag cggttccatc ctctagagga
2521 tagaatggcg ccgggccttt ctttatgttt ttggcgtctt ccattttacc aacagtaccg
2581 gaatgccaag ctcgagctca gggtcattaa ttgtaaagga aaggtgccga cctccggtca
2641 cctattcagg cgtagaagct ttttaaaccg agcgctttta tagaagcccc agtccccttc
2701 tcagatcccg agtgtcgcgt gttacctcgg ggtacctgaa gggcatcctt agcctgtcac
2761 cagtgtgtgg gtaccagttt caatgtcact ttaaagtggg tttcgcagta aagtcgccgt
2821 gctggggttg cacacatgac gggcccgtgt gggcgtggct tttggccccg tgggtccttg
2881 ttaccaagga ggagttccca cggccgtcat gctccgctac gaagcaacag tgctgttact
2941 aacactcgcc agtggatcga gcgtggagcc aaacacagta caaagtgtta cccctctaga
3001 cctggaaaga ccaggcggag tttcaagttg cacgttttgt gtcagttggg gaaacctgct
3061 gagctctgcg gcacgctgtt gacgctgtta agcgggtcgc tgcagcaata actgccttaa
3121 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
3181 ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc
3241 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
3301 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
3361 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
3421 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
3481 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
3541 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
3601 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
3661 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
3721 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
3781 tccattttag cttctgcagg gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg
3841 cgaagtggac ctcggaccgc gccgccccga ctgcatctgc gtgttcgaat tcgccaatga
3901 caagacgctg ggcggggttt gccggatcct ctagagtcga gcttgggtct ccctatagtg
3961 agtcgtatta atttcgataa gccagctgca ttaatgaatc ggccaacgcg cggggagagg
4021 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
4081 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
4141 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
4201 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
4261 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
4321 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
4381 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
4441 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
4501 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
4561 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
4621 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
4681 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
4741 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
4801 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
4861 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
4921 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
4981 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
5041 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
5101 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
5161 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
5221 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
5281 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
5341 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
5401 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
5461 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
5521 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
5581 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
5641 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
5701 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
5761 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
5821 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
5881 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
5941 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
6001 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
6061 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
6121 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
6181 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatcg acgctctccc
6241 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
6301 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
6361 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
6421 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgcc
//