LOCUS LT727336 6561 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF1mtBIDHis, complete sequence.
ACCESSION LT727336
VERSION LT727336.1
KEYWORDS .
SOURCE Mammalian expression vector pEF1mtBIDHis
ORGANISM Mammalian expression vector pEF1mtBIDHis
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6561)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6561
/organism="Mammalian expression vector pEF1mtBIDHis"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4323."
/db_xref="taxon:1944986"
regulatory 508..1651
/note="hEF1"
/regulatory_class="promoter"
misc_feature 704..1642
/note="intron"
regulatory 1668..1684
/note="T7"
/regulatory_class="promoter"
CDS <1757..>2164
/codon_start=1
/transl_table=11
/note="mtBID"
/protein_id="SJL88145.1"
/translation="GSQASRSFNQGRIEPDSESQEEIIHNIARHLAQIGDEMDHNIQP
TLVRQLAAQFMNGSLSEEDKRNCLAKALDEVKTAFPRDMENDKAMLIMTMLLAKKVAS
HAPSLLRDVFHTTVNFINQNLFSYVRNLVRNEMD"
misc_feature 2192..2233
/note="V5"
misc_feature 2243..2260
/note="H6"
regulatory 2289..2513
/note="polyA"
/regulatory_class="terminator"
rep_origin 2559..2987
/note="f1 ori"
regulatory 2992..3273
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3230..3305
/note="SV40 ori"
CDS 3397..4191
/transl_table=11
/note="neomycin resistance"
/protein_id="SJL88146.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 4367..4441
/note="polyA"
/regulatory_class="terminator"
regulatory complement(4367..4441)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(4755..5146)
/note="pMB1 ori"
CDS complement(5687..6547)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88147.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1452 a 1710 c 1771 g 1628 t
ORIGIN
1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag
1741 tgtggtggaa ttcatgggca gccaggccag ccgctccttc aaccaaggaa gaatagagcc
1801 agattctgaa agtcaggaag aaatcatcca caacattgcc agacatctcg cccaaatagg
1861 cgatgagatg gaccacaaca tccagcccac actggtgaga cagctagccg cacagttcat
1921 gaatggcagc ctgtcggagg aagacaaaag gaactgcctg gccaaagccc ttgatgaggt
1981 gaagacagcc ttccccagag acatggagaa cgacaaggcc atgctgataa tgacaatgct
2041 gttggccaaa aaagtggcca gtcacgcacc atctttgctc cgtgatgtct tccacacgac
2101 tgtcaacttt attaaccaga acctattctc ctatgtgagg aacttggtta gaaacgagat
2161 ggactgggcc gctcgaggtc acccattcga aggtaagcct atccctaacc ctctcctcgg
2221 tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
2281 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
2341 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
2401 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
2461 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
2521 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
2581 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
2641 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
2701 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
2761 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
2821 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
2881 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
2941 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
3001 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
3061 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
3121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
3181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
3241 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
3301 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
3361 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
3421 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
3481 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
3541 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
3601 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
3661 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
3721 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
3781 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
3841 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
3901 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
3961 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
4021 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
4081 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
4141 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
4201 tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
4261 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
4321 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
4381 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
4441 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
4501 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
4561 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
4621 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
4681 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
4741 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
4801 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
4861 ggataacgca ggaaagaaca tgtgagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
4921 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
4981 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
5041 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
5101 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
5161 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
5221 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
5281 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
5341 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
5401 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
5461 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
5521 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
5581 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
5641 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
5701 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
5761 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
5821 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
5881 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
5941 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
6001 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
6061 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
6121 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
6181 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
6241 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
6301 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
6361 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
6421 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
6481 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
6541 tactcatact cttccttttt c
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