LOCUS LT727334 9534 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pAS3, complete sequence.
ACCESSION LT727334
VERSION LT727334.1
KEYWORDS .
SOURCE Yeast expression vector pAS3
ORGANISM Yeast expression vector pAS3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9534)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9534
/organism="Yeast expression vector pAS3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4253."
/db_xref="taxon:1945466"
rep_origin complement(2..982)
/note="2mu ori"
regulatory complement(1060..1164)
/note="FLP terminator"
/regulatory_class="terminator"
CDS 3477..5192
/transl_table=11
/note="ADE2"
/protein_id="SJL88138.1"
/translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISN
SNDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRL
IQDKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNF
VVKNKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNI
CDLCYAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHN
SGHYTIDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELET
CERALATPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVA
QKLDLEAMVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYA
ISASKRGIKTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRG
VPVATVAINNSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEA
YLENK"
regulatory 6196..6599
/note="ADH1 promoter"
/regulatory_class="promoter"
CDS 6622..>7062
/transl_table=11
/note="GALbd"
/protein_id="SJL88139.1"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
misc_feature 7078..7104
/note="HA epitope"
regulatory 7172..7273
/note="ADH1 terminator"
/regulatory_class="terminator"
regulatory complement(7426..7509)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(7601..8003)
/note="pMB1 ori"
CDS complement(8544..9404)
/transl_table=11
/note="ampicillin"
/protein_id="SJL88140.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2820 a 1970 c 1965 g 2777 t
ORIGIN
1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt
1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca
1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt
1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc
1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact
1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc
1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta
1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat
1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac
1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca
1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa
1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta
1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat
1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg
1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg
2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact
2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg
2161 gcattgatnc cacggtgtta tagtatcagc aatagatctt tttactcaaa tttacagttt
2221 tgatatcttg gcgtttttcc tcacatttat tacaaaagaa agtgggcagg atttgccctt
2281 cacaaaatct ctgtccagga gaagaacgta tttgaccaga attgctcaat cttcttcatt
2341 gagacagttt ttacaactga gtacacgccc taatataagg attctgggag gtaatgggca
2401 gggtactgga aattccaatt atcccgaatt tacaaacgca tcctcagtca tctcacccaa
2461 gctaagggct tctcctctac tagaaagaag ggcgtcaaaa atctgctatg ctattacgaa
2521 attactctat aggttagaat gtcatcccat aggtatggcc cttttaaaga agtatctcca
2581 caatcaattg cgagaagcct atctagaatc aaaacgacac tttatttcca aaaagggaga
2641 ttcgaccaac acttcctcta ccattgcatc atcatctttc gctggcgcat ctgttcctct
2701 atcttcaaac gaatcaggaa tgctaaacgg cttgaagcaa attaacgaac aacaagaatc
2761 tacattagaa accactcaaa aggaagacta gtaacgccgt atcgtgatta acgtattaca
2821 taagttacag gattcatgct tatgggttag ctatttcgcc caatgtgtcc atctgacatt
2881 actattttgc attttaattt aattagaact tgactagcgc actaccagta tatcatctca
2941 tttccgtaaa taccaaatgt attatatatt gaaagctttt gaccaggtta ttataaaaga
3001 aacttcatgc tcgaaaaaga tcatttcgaa aagttgccta gtttcatgaa attttaaagc
3061 agtttatata aattttacct tttgatgcgg aattgacttt ttcttgaata atacataact
3121 tttcttaaaa gaatcaaaga cagataaaat ttaagagata ttaaatatta gtgagaagcc
3181 gagaattttg taacaccaac ataacactga catctttaac aacttttaat tatgatacat
3241 ttcttacgtc atgattgatt attacagcta tgctgacaaa tgactcttgt tgcatggcta
3301 cgaaccgggt aatactaagt gattgactct tgctgacctt ttattaagaa ctaaatggac
3361 aatattatgg agcatttcat gtataaattg gtgcgtaaaa tcgttggatc tctcttctaa
3421 gtacatccta ctataacaat caagaaaaac aagaaaatcg gacaaaacaa tcaagtatgg
3481 attctagaac agttggtata ttaggagggg gacaattggg acgtatgatt gttgaggcag
3541 caaacaggct caacattaag acggtaatac tagatgctga aaattctcct gccaaacaaa
3601 taagcaactc caatgaccac gttaatggct ccttttccaa tcctcttgat atcgaaaaac
3661 tagctgaaaa atgtgatgtg ctaacgattg agattgagca tgttgatgtt cctacactaa
3721 agaatcttca agtaaaacat cccaaattaa aaatttaccc ttctccagaa acaatcagat
3781 tgatacaaga caaatatatt caaaaagagc atttaatcaa aaatggtata gcagttaccc
3841 aaagtgttcc tgtggaacaa gccagtgaga cgtccctatt gaatgttgga agagatttgg
3901 gttttccatt cgtcttgaag tcgaggactt tggcatacga tggaagaggt aacttcgttg
3961 taaagaataa ggaaatgatt ccggaagctt tggaagtact gaaggatcgt cctttgtacg
4021 ccgaaaaatg ggcaccattt actaaagaat tagcagtcat gattgtgaga tctgttaacg
4081 gtttagtgtt ttcttaccca attgtagaga ctatccacaa ggacaatatt tgtgacttat
4141 gttatgcgcc tgctagagtt ccggactccg ttcaacttaa ggcgaagttg ttggcagaaa
4201 atgcaatcaa atcttttccc ggttgtggta tatttggtgt ggaaatgttc tatttagaaa
4261 caggggaatt gcttattaac gaaattgccc caaggcctca caactctgga cattatacca
4321 ttgatgcttg cgtcacttct caatttgaag ctcatttgag atcaatattg gatttgccaa
4381 tgccaaagaa tttcacatct ttctccacca ttacaacgaa cgccattatg ctaaatgttc
4441 ttggagacaa acatacaaaa gataaagagc tagaaacttg cgaaagagca ttggcgactc
4501 caggttcctc agtgtactta tatggaaaag agtctagacc taacagaaaa gtaggtcaca
4561 taaatattat tgcctccagt atggcggaat gtgaacaaag gctgaactac attacaggta
4621 gaactgatat tccaatcaaa atctctgtcg ctcaaaagtt ggacttggaa gcaatggtca
4681 aaccattggt tggaatcatc atgggatcag actctgactt gccggtaatg tctgccgcat
4741 gtgcggtttt aaaagatttt ggcgttccat ttgaagtgac aatagtctct gctcatagaa
4801 ctccacatag gatgtcagca tatgctattt ccgcaagcaa gcgtggaatt aaaacaatta
4861 tcgctggagc tggtggggct gctcacttgc caggtatggt ggctgcaatg acaccacttc
4921 ctgtcatcgg tgtgcccgta aaaggttctt gtctagatgg agtagattct ttacattcaa
4981 ttgtgcaaat gcctagaggt gttccagtag ctaccgtcgc tattaataat agtacgaacg
5041 ctgcgctgtt ggctgtcaga ctgcttggcg cttatgattc aagttataca acgaaaatgg
5101 aacagttttt attaaagcaa gaagaagaag ttcttgtcaa agcacaaaag ttagaaactg
5161 tcggttacga agcttatcta gaaaacaagt aatatataag tttattgata tacttgtaca
5221 gcaaataatt ataaaatgat atacctattt tttaggcttt gttatgatta catcaaatgt
5281 ggacttcata catagaaatc aacgcttaca ggtgtccttt tttaagaatt tcatacataa
5341 gatcacttat tatacataca tacatatcca gtaacaagaa gcaaggaata attacctgct
5401 taagtctgcg attaaaaaaa taacgtttcg atacagttca tataaggcgg ctcaatgcag
5461 aaccgaggat agcgctacgt caggatatct ttgtagttcc caaatataaa tgcgacaata
5521 tagtttcttt ctttcatatc aataatatcc ttttctccac tgaaatcacg aatcaaacct
5581 ggagcaaaaa ctaaagccaa attataaagc gtcattcgat tccagtgact gtaccgtgta
5641 accttttcta tatgttcact cagtactctt aacaccctat aatgttccct tggaaggtct
5701 tccaatatgt tttttaaagc gctcttgctc gacatataag tgtccgaatt ctttgcttct
5761 aaggacaact ttcctccaac aaatggcaag ttttccatca tttttttaga tttaactaac
5821 ctcatcaacg gctcgtatat ttgaaaggta aagatagggt tggggagctt tcttaagtat
5881 cgcttcaana tccttttgtt gtttccgggt gtacaatatg gacttcctct tttctggcaa
5941 ccaaacccat acatcgggat tcctataata ccttcgttgg tctccctaac atgtaggtgg
6001 cggaggggag atatacaata gaacagatac cagacaagac ataatgggct aaacaagact
6061 acaccaatta cactgcctca ttgatggtgg tacataacga actaatactg tagccctaga
6121 cttgatagcc atcatcatat cgaagtttca ctaccctttt tccatttgcc atctattgaa
6181 gtaataatag gcgcatgcaa cttcttttct ttttttttct tttctctctc ccccgttgtt
6241 gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga cactaaagga aaaaattaac
6301 gacaaagaca gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca
6361 cacgaaactt tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg
6421 gccttccttc cagttacttg aatttgaaat aaaaaaagtt tgccgctttg ctatcaagta
6481 taaatagacc tgcaattatt aatcttttgt ttcctcgtca ttgttctcgt tccctttctt
6541 ccttgtttct ttttctgcac aatatttcaa gctataccaa gcatacaatc aactccaagc
6601 ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta tcgaacaagc atgcgatatt
6661 tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag
6721 aacaactggg agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat
6781 ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct
6841 cgagaagacc ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta
6901 acaggattat ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca
6961 gtggagactg atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg
7021 gaagagagta gtaacaaagg tcaaagacag ttgactgtat cgccggaatt catggcttac
7081 ccatacgatg ttccagatta cgctagcttg ggtggtcata tggccatgga ggccccgggg
7141 atccgtcgac ctgcagccaa gctaattccg ggcgaatttc ttatgattta tgatttttat
7201 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
7261 aaaacgaaaa ttcttgttct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
7321 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgcaagctt ggcgtaatca
7381 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
7441 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt
7501 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct ggattaatga
7561 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
7621 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
7681 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
7741 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
7801 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
7861 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
7921 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
7981 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
8041 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
8101 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
8161 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
8221 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
8281 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
8341 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
8401 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
8461 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
8521 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
8581 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
8641 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
8701 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
8761 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
8821 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
8881 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
8941 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
9001 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
9061 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
9121 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
9181 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
9241 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
9301 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
9361 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
9421 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
9481 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acct
//