LOCUS LT727333 8796 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pACCMVpLpA, complete sequence.
ACCESSION LT727333
VERSION LT727333.1
KEYWORDS .
SOURCE Mammalian expression vector pACCMVpLpA
ORGANISM Mammalian expression vector pACCMVpLpA
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8796)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8796
/organism="Mammalian expression vector pACCMVpLpA"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4235."
/db_xref="taxon:1944745"
rep_origin 1..454
/note="Ad5 ori"
regulatory 673..1256
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
CDS <1346..1420
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88136.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 1421..1487
/note="intron of SV40 small t-antigen"
regulatory 1625..1721
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1625..1721)
/note="SV40 polyA late"
/regulatory_class="terminator"
misc_feature 1795..4697
/note="Ad5 flanking np3334-6236"
rep_origin complement(6778..7180)
/note="pMB1 ori"
CDS complement(7721..8581)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88137.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1960 a 2216 c 2402 g 2218 t
ORIGIN
1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg
361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc
421 cgggtcaaag ttggcgtttt attattatag tcagtctagg cggccgcgat ctatacattg
481 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
541 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
601 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
661 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
721 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
781 atagtaacgc caatagggac tttccattga cgtcaataat gacgtatgtt cccatagtaa
841 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
901 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta
961 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt
1021 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg
1081 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
1141 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc
1201 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
1261 tagtgaaccg tcagatccgg tcgcgcgaat tcgagctcgg tacccgggga tcctctagag
1321 tcgacctgca ggcatgcaag cttgggatct ttgtgaagga accttacttc tgtggtgtga
1381 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
1441 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt cacagtccca
1501 aggctcattt caggcccctc agtcctcaca gtctgttcat gatcataatc agccatacca
1561 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
1621 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
1681 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
1741 gtttgtccaa actcatcaat gtatcttatc atgtctggat cgcggccgcc tagaggaagg
1801 tgctgaggta cgatgagacc cgcaccaggt gcagaccctg cgagtgtggc ggtaaacata
1861 ttaggaacca gcctgtgatg ctggatgtga ccgaggagct gaggcccgat cacttggtgc
1921 tggcctgcac ccgcgctgag tttggctcta gcgatgaaga tacagattga ggtactgaaa
1981 tgtgtgggcg tggcttaagg gtgggaaaga atatataagg tgggggtctt atgtagtttt
2041 gtatctgttt tgcagcagcc gccgccgcca tgagcaccaa ctcgtttgat ggaagcattg
2101 tgagctcata tttgacaacg cgcatgcccc catgggccgg ggtgcgtcag aatgtgatgg
2161 gctccagcat tgatggtcgc cccgtcctgc ccgcaaactc tactaccttg acctacgaga
2221 ccgtgtctgg aacgccgttg gagactgcag cctccgccgc cgcttcagcc gctgcagcca
2281 ccgcccgcgg gattgtgact gactttgctt tcctgagccc gcttgcaagc agtgcagctt
2341 cccgttcatc cgcccgcgat gacaagttga cggctctttt ggcacaattg gattctttga
2401 cccgggaact taatgtcgtt tctcagcagc tgttggatct gcgccagcag gtttctgccc
2461 tgaaggcttc ctcccctccc aatgcggttt aaaacataaa taaaaaacca gactctgttt
2521 ggatttggat caagcaagtg tcttgctgtc tttatttagg ggttttgcgc gcgcggtagg
2581 cccgggacca gcggtctcgg tcgttgaggg tcctgtgtat tttttccagg acgtggtaaa
2641 ggtgactctg gatgttcaga tacatgggca taagcccgtc tctggggtgg aggtagcacc
2701 actgcagagc ttcatgctgc ggggtggtgt tgtagatgat ccagtcgtag caggagcgct
2761 gggcgtggtg cctaaaaatg tctttcagta gcaagctgat tgccaggggc aggcccttgg
2821 tgtaagtgtt tacaaagcgg ttaagctggg atgggtgcat acgtggggat atgagatgca
2881 tcttggactg tatttttagg ttggctatgt tcccagccat atccctccgg ggattcatgt
2941 tgtgcagaac caccagcaca gtgtatccgg tgcacttggg aaatttgtca tgtagcttag
3001 aaggaaatgc gtggaagaac ttggagacgc ccttgtgacc tccaagattt tccatgcatt
3061 cgtccataat gatggcaatg ggcccacggg cggcggcctg ggcgaagata tttctgggat
3121 cactaacgtc atagttgtgt tccaggatga gatcgtcata ggccattttt acaaagcgcg
3181 ggcggagggt gccagactgc ggtataatgg ttccatccgg cccaggggcg tagttaccct
3241 cacagatttg catttcccac gctttgagtt cagatggggg gatcatgtct acctgcgggg
3301 cgatgaagaa aacggtttcc ggggtagggg agatcagctg ggaagaaagc aggttcctga
3361 gcagctgcga cttaccgcag ccggtgggcc cgtaaatcac acctattacc gggtgcaact
3421 ggtagttaag agagctgcag ctgccgtcat ccctgagcag gggggccact tcgttaagca
3481 tgtccctgac tcgcatgttt tccctgacca aatccgccag aaggcgctcg ccgcccagcg
3541 atagcagttc ttgcaaggaa gcaaagtttt tcaacggttt gagaccgtcc gccgtaggca
3601 tgcttttgag cgtttgacca agcagttcca ggcggtccca cagctcggtc acctgctcta
3661 cggcatctcg atccagcata tctcctcgtt tcgcgggttg gggcggcttt cgctgtacgg
3721 cagtagtcgg tgctcgtcca gacgggccag ggtcatgtct ttccacgggc gcagggtcct
3781 cgtcagcgta gtctgggtca cggtgaaggg gtgcgctccg ggctgcgcgc tggccagggt
3841 gcgcttgagg ctggtcctgc tggtgctgaa gcgctgccgg tcttcgccct gcgcgtcggc
3901 caggtagcat ttgaccatgg tgtcatagtc cagcccctcc gcggcgtggc ccttggcgcg
3961 cagcttgccc ttggaggagg cgccgcacga ggggcagtgc agacttttga gggcgtagag
4021 cttgggcgcg agaaataccg attccgggga gtaggcatcc gcgccgcagg ccccgcagac
4081 ggtctcgcat tccacgagcc aggtgagctc tggccgttcg gggtcaaaaa ccaggtttcc
4141 cccatgcttt ttgatgcgtt tcttacctct ggtttccatg agccggtgtc cacgctcggt
4201 gacgaaaagg ctgtccgtgt ccccgtatac agacttgaga ggcctgtcct cgagcggtgt
4261 tccgcggtcc tcctcgtata gaaactcgga ccactctgag acaaaggctc gcgtccaggc
4321 cagcacgaag gaggctaagt gggaggggta gcggtcgttg tccactaggg ggtccactcg
4381 ctccagggtg tgaagacaca tgtcgccctc ttcggcatca aggaaggtga ttggtttgta
4441 ggtgtaggcc acgtgaccgg gtgttcctga aggggggcta taaaaggggg tgggggcgcg
4501 ttcgtcctca ctctcttccg catcgctgtc tgcgagggcc agctgttggg gtgagtactc
4561 cctctgaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa acgaggagga
4621 tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcatcca tctggtcaga
4681 aaagacaatc tttttgttgt caagctagct tctatgggtt taacaatacc tgtgtggaag
4741 cctggaccga tgtaagggtt cggggctgtg ccttttactg ctgctggaag ggggtggtgt
4801 gtcgccccaa aagcagggct tcaattaaga aatgcctctt tgaaaggtgt accttgggta
4861 tcctgtctga gggtaactcc agggtgcgcc acaatgtggc ctccgactgt ggttgcttca
4921 tgctagtgaa aagcgtggct gtgattaagc ataacatggt atgtggcaac tgcgaggaca
4981 gggcctctca gatgctgacc tgctcggacg gcaactgtca cctgctgaag accattcacg
5041 tagccagcca ctctcgcaag gcctggccag tgtttgagca taacatactg acccgctgtt
5101 ccttgcattt gggtaacagg aggggggtgt tcctacctta ccaatgcaat ttgagtcaca
5161 ctaagatatt gcttgagccc gagagcatgt ccaaggtgaa cctgaacggg gtgtttgaca
5221 tgaccatgaa gatctgcgtc tccggtctcg ccgcttagat cgctctgtgt agtagttgta
5281 gtatatccac tctctcaaag catccaggcg ccccctggct tcgggttcta tgtaaactcc
5341 ttcatgcgcc gctgccctga taacatccac caccgcagaa taagccacac ccagccaacc
5401 tacacattcg ttctgcgagt cacacacggg aggagcggga agagctggaa gaaccatgtt
5461 ttttttttta ttccaaaaga ttatccaaaa cctcaaaatg aagatcctct acgccggacg
5521 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
5581 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
5641 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
5701 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
5761 gtcgcataag ggagagcgtc gatcgaccga tgcccttgag agccttcaac ccagtcagct
5821 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
5881 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
5941 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
6001 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg
6061 ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga
6121 tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg
6181 ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg
6241 ctcttaccag cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct
6301 cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc
6361 tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg
6421 gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac
6481 tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag
6541 ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct
6601 cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc
6661 accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac
6721 aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgct
6781 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
6841 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
6901 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
6961 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
7021 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
7081 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
7141 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
7201 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
7261 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
7321 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
7381 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
7441 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
7501 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
7561 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
7621 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
7681 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
7741 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
7801 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
7861 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
7921 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
7981 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
8041 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
8101 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
8161 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
8221 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
8281 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
8341 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
8401 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
8461 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
8521 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
8581 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
8641 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
8701 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
8761 gtatcacgag gccctttcgt cttcaagaat taattc
//