LOCUS LT727332 6882 bp DNA circular SYN 06-FEB-2017
DEFINITION Reporter vector pIgK3conalacZ, complete sequence.
ACCESSION LT727332
VERSION LT727332.1
KEYWORDS .
SOURCE Reporter vector pIgK3conalacZ
ORGANISM Reporter vector pIgK3conalacZ
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6882)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6882
/organism="Reporter vector pIgK3conalacZ"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4234."
/db_xref="taxon:1945221"
rep_origin complement(3..458)
/note="f1ori"
regulatory 626..642
/note="T7"
/regulatory_class="promoter"
regulatory 953..1005
/note="IgK3"
/regulatory_class="promoter"
regulatory 1009..1172
/note="CONA"
/regulatory_class="promoter"
CDS 1258..>1293
/transl_table=11
/note="NLS"
/protein_id="SJL88133.1"
/translation="MDPKKKRKVEDP"
CDS <1315..4362
/codon_start=1
/transl_table=11
/note="lacZ"
/protein_id="SJL88134.1"
/translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVN
PPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLP
SEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH
VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGG
YADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLL
LLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYT
LCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSL
GNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQS
LIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLS
GQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPES
AGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFC
IELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAW
VERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQM
AITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPL
SDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEE
GTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
regulatory 4492..4584
/note="polyA"
/regulatory_class="terminator"
regulatory complement(4492..4584)
/note="polyA"
/regulatory_class="terminator"
regulatory complement(4775..4854)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(4951..5353)
/note="pMB1 ori"
CDS complement(5894..6754)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88135.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1656 a 1808 c 1842 g 1576 t
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
661 gccccccctc gaggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
721 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
781 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
841 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
901 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctgggga
961 ttccccatga tctggggatt ccccatgatc tggggattcc ccagatccct attgagaaac
1021 aaggaaggac aaacagcgtt aggtcattgc ttctgcaaac acagccaggg ctgctcctct
1081 ataaaagggg aagaaagagg ctccgcagcc atcacagacc cagaggggac ggtctgtgac
1141 caacaccgct gctccccctc ctcaacaccc aggatcgatc ctattccaga agtagtgaag
1201 aggctttttt ggaggcctag gcttttgcaa aaagcttgat atcgaattcc tgcagccatg
1261 gatccaaaaa agaagagaaa ggtagaagac ccccgcatgg ctcgcgatga tcccgtcgtt
1321 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
1381 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
1441 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
1501 gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
1561 cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat tacggtcaat
1621 ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
1681 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa ctcggcgttt
1741 catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt gccgtctgaa
1801 tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgt
1861 tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt
1921 gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt tgccactcgc
1981 tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg cggcgagttg
2041 cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt cgccagcggc
2101 accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga tcgcgtcaca
2161 ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa tctctatcgt
2221 gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc ctgcgatgtc
2281 ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg
2341 attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat ggatgagcag
2401 acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt gcgctgttcg
2461 cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg
2521 gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac cgatgatccg
2581 cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac
2641 ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa tcacgacgcg
2701 ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga
2761 gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag
2821 cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc tggagagacg
2881 cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg cggtttcgct
2941 aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt ctgggactgg
3001 gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc ttacggcggt
3061 gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt ctttgccgac
3121 cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt ccagttccgt
3181 ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag cgataacgag
3241 ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga agtgcctctg
3301 gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca gccggagagc
3361 gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa
3421 gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc
3481 cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt ttgcatcgag
3541 ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca gatgtggatt
3601 ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc accgctggat
3661 aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt cgaacgctgg
3721 aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc agatacactt
3781 gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa aaccttattt
3841 atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac cgttgatgtt
3901 gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca gctggcgcag
3961 gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc cgaccgcctt
4021 actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac cccgtacgtc
4081 ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg cccacaccag
4141 tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact gatggaaacc
4201 agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga cggtttccat
4261 atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt ccagctgagc
4321 gccggtcgct accattacca gttggtctgg tgtcaaaaat aataataacc gggcaggggg
4381 gatctaagct ctagataagt aatgatcata atcagccata tcacatctgt agaggtttta
4441 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
4501 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
4561 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
4621 aatgtatctt atcatgtctg gatccactag ttctagagcg gccgccaccg cggtggagct
4681 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
4741 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
4801 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
4861 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
4921 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
4981 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
5041 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
5101 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
5161 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
5221 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
5281 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
5341 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
5401 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
5461 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
5521 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
5581 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
5641 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
5701 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
5761 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
5821 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
5881 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
5941 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
6001 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
6061 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
6121 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
6181 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
6241 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
6301 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
6361 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
6421 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
6481 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
6541 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
6601 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
6661 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
6721 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
6781 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
6841 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//