LOCUS LT727328 7258 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pCaMET15, complete sequence.
ACCESSION LT727328
VERSION LT727328.1
KEYWORDS .
SOURCE Yeast expression vector pCaMET15
ORGANISM Yeast expression vector pCaMET15
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7258)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7258
/organism="Yeast expression vector pCaMET15"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4218."
/db_xref="taxon:1945467"
regulatory complement(12..28)
/note="SP6 promoter"
/regulatory_class="promoter"
regulatory complement(89..172)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(264..666)
/note="pMB1 ori"
CDS complement(1207..2067)
/transl_table=11
/note="ampicillin"
/protein_id="SJL88124.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin complement(2506..2961)
/note="f1 ori"
regulatory 3125..3141
/note="T7 promoter"
/regulatory_class="promoter"
CDS 3692..4504
/transl_table=11
/note="CaURA3"
/protein_id="SJL88125.1"
/translation="MTVNTKTYSERAETHASPVAQRLFRLMELKKTNLCASIDVDTTK
EFLELIDKLGPYVCLIKTHIDIINDFSYESTIEPLLELSRKHQFMIFEDRKFADIGNT
VKKQYIGGVYKISSWADITNAHGVTGNGVVEGLKQGAKETTTNQEPRGLLMLAELSSV
GSLAYGEYSQKTVEIAKSDKEFVIGFIAQRDMGGQEEGFDWLIMTPGVGLDDKGDGLG
QQYRTVDEVVSTGTDIIIVGRGLFGKGRDPDIEGKRYRNAGWNAYLKKTGQL"
misc_feature complement(4804..5115)
/note="3' UTR of CaMET15, incl. polyA"
CDS complement(5116..6438)
/transl_table=11
/note="CaMET15"
/protein_id="SJL88126.1"
/translation="MPSHFDTLQLHAGQPVEKPHQPRAPPIYATTSYVFNDSKHGAQL
FGLETPGYIYSRIMNPTNDVFEQRIAALEGGIGALATSSGQSAQFLAIAGLAHAGDNI
ISTSYLYGGTYNQFKVAFKRLGIETKFVNGDAAEDFAKLIDDKTKAIYIETIGNPKYN
VPDFEKITKLAHEHGIPVVVDNTFGAGGFLVNPIAHGADIVVHSATKWIGGHGTTIAG
VIVDSGNFPWTEYPEKYPQFSKPSEGYHGLVLNDALGKAAYIGHLRIELLRDLGPALN
PFGSFLLLQGLETLSLRVERQSENALKLAQWLEKNPNVESVSYLGLPSHESHELSKKY
LNNDAKYFGGALAFTVKDITNTSSDPFNEASPKLVDNLEIASNLANVGDSKTLVIAPW
FTTHQQLSDEEKLASGVTKGLIRVSTGTEYIDDIINDFEQAFKKVYNN"
regulatory complement(6439..6979)
/note="CaMET15 promoter"
/regulatory_class="promoter"
regulatory 6988..7258
/note="CYC1 terminator"
/regulatory_class="terminator"
BASE COUNT 2143 a 1477 c 1583 g 2055 t
ORIGIN
1 cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc
61 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
121 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
181 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
241 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
301 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
361 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
421 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
481 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
541 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
601 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
661 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
721 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
781 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
841 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
901 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
961 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
1021 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
1081 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
1141 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
1201 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
1261 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
1321 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
1381 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
1441 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
1501 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
1561 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
1621 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
1681 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
1741 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
1801 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
1861 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
1921 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
1981 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
2041 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
2101 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
2161 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
2221 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
2281 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
2341 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
2401 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
2461 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgaaatt gtaaacgtta
2521 atattttgtt aaaattcgcg ttaaatattt gttaaatcag ctcatttttt aaccaatagg
2581 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
2641 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
2701 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgc
2761 ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga tttagagctt
2821 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
2881 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
2941 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
3001 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
3061 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
3121 attgtaatac gactcactat agggcgaatt ggttttccaa tgatgagcac ttttaaagtt
3181 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
3241 atacactatt ctcagaatga cttggttgag tactaatagg aattgatttg gatggtataa
3301 acggaaacaa aaaaaagagc tggtactact ttctttaaaa ttattttatt atttgatttt
3361 atttaatagt atatattata ttttgaacgt agattatttt gttgaaagtt gctgtagtgc
3421 cattgattcg taacactaat tctgtattag tcattcctct tgtttgatag tatccaaaaa
3481 aacggctatt tttttgcaat cttatttcct gcatattata cagataacat aatgaaagaa
3541 aaaatctttt tttttgttct tcaatgatga tttcaaccat tcttttaaac attgatcaat
3601 tcctgagcaa caaccccata cacactggtt tatataccgc cccttttaca gttgaagaaa
3661 gaaatagaaa tagaaatagc aaacaaaaga tatgacagtc aacactaaga cctatagtga
3721 gagagcagaa actcatgcct caccagtagc acagcgatta tttcgattaa tggaactgaa
3781 gaaaaccaat ttatgtgcat caattgacgt tgataccact aaggaattcc ttgaattaat
3841 tgataaatta ggtccttatg tatgcttaat caagactcat attgatataa tcaatgattt
3901 ttcctatgaa tccactattg aaccattatt agaactttca cgtaaacatc aatttatgat
3961 ttttgaagat agaaaatttg ctgatattgg taataccgta aagaaacaat atattggtgg
4021 agtttataaa attagtagtt gggcagatat taccaatgct catggtgtca ctgggaatgg
4081 agtggttgaa ggattaaaac agggagctaa agaaaccacc accaaccaag agccaagagg
4141 gttattgatg ttagctgaat tatcatcagt gggatcatta gcatatggag aatattctca
4201 aaaaactgtt gaaattgcta aatccgataa ggaatttgtt attggattta ttgcccaacg
4261 tgatatgggt ggccaagaag aaggatttga ttggcttatt atgacacctg gagttggatt
4321 agatgataaa ggtgatggat taggacaaca atatagaact gttgatgaag ttgttagcac
4381 tggaactgat attatcattg ttggtagagg attgtttggt aaaggaagag atccagatat
4441 tgaaggtaaa aggtatagaa atgctggttg gaatgcttat ttgaaaaaga ctggccaatt
4501 ataaatgtga agggggagat tttcacttta ttagatttgt atatatgtag aataaataaa
4561 taaataagtt aaataaataa ttaaataagg gtggtaatta ttactattta caatcaaagg
4621 tggtccttct agctgtaatc cgggcagcgc aacggaacat tcatcagtgt aaaaatggaa
4681 tcaataaagc cctgcgcagc gcgcagggtc agcctgaata cgcgtttaat gaccagcaca
4741 gtcgtgatgg caaggtcaga atagcccaag tcggccgagg ggcctgtaca gtgagggaag
4801 atcgatctca tctttgattt gcttagctgt gactaccgct acctcttttt ttgaaatgat
4861 aggttactgt gtttttgcaa aatctttggc tagagtcttt ttcattgtca aggtcatatc
4921 tatgttggtt ttcgtcttca caatatgtat actatactgc ataatcatgg ttatgaaaaa
4981 ctttttaacc gatttaatca cccaatttca aattggaacc ctagagttcc cctccatcaa
5041 atggattact tggggtgttt taacattaat gtgtctaatt attgttgtga aacgttatct
5101 tatataccaa tctatttagt tgttataaac cttcttgaat gcttgttcaa agtcgttaat
5161 aatatcatca atatattcag taccagtaga aactctgatt aagcccttgg taacaccaga
5221 agccaacttt tcttcatcag acaattgttg atgtgtagta aaccatggag caataaccaa
5281 agtcttagag tcacccacat tagccaagtt tgaagcaatc tccaaattgt caaccaactt
5341 tggtgaggct tcattgaatg ggtcgctgga ggtgttggtg atgtccttga cagtaaatgc
5401 taaagcacca ccaaagtact tagcgtcatt gttcaagtat tttttactca attcgtggga
5461 ttcgtgagat ggcaatccca aataggacac agactcaaca tttgggttct tttctaacca
5521 ttgggccaat ttcaaagcat tttcagattg tctttcaact ctcaaagaca aagtttccaa
5581 accttgcaac aacaaaaaac ttccaaatgg attcaaagct ggacccaagt ctctcaacaa
5641 ttcaattctc aagtgaccaa tgtatgcggc cttacctaaa gcatcattca agaccaaccc
5701 gtggtaacct tctgatggtt tagagaattg tgggtatttt tctgggtact cggtccatgg
5761 gaagttaccg gaatcaacaa taacaccagc aattgtagta ccgtgaccac caatccattt
5821 agtagcagaa tgaacaacaa tatcagcacc gtgggcaatt gggttaacta aaaatccacc
5881 agcaccaaaa gtgttgtcga caacaacagg aataccgtgt tcatgggcca atttggtgat
5941 tttttcaaag tctggaacat tatatttagg gtttccaatg gtttcaatat aaatagcttt
6001 tgtcttgtcg tcaatcaatt tagcaaaatc ttcagcggcg tcaccattaa cgaatttggt
6061 ttcaatgccc aaacgtttga aagcaacttt gaattgatta taagtaccac catacaagta
6121 ggatgtactg ataatgttat caccagcatg agccaaccca gcaatagcca agaattgagc
6181 tgattgacca gaagaagtgg ccaatgcacc aataccacct tccaaggcag caattctttg
6241 ttcaaacaca tcgtttgttg gattcataat tctggagtaa atgtatcctg gggtttctaa
6301 accaaataat tgagcaccgt gtttagagtc attgaaaaca taggaggtgg ttgcataaat
6361 tggtggggct cttggttggt gtggtttttc aactggttgc ccagcatgta attgaagtgt
6421 atcaaagtga gaaggcattg tagtttagta atggggaaaa caaaaaagga agaattaaaa
6481 caaaaaaaaa aagtggctct aaagaatatt tatatttgtg aaaaacgtcg attcgtatag
6541 gctatttata ttatctcaaa ataatttcat catggaccga gaactgtgga tgcaaagcct
6601 ataaactttt aaccaccaca ggaggaaaag tcaataacgc ggagtgaatt gagggacaac
6661 aaaaaaaaac tgttaccatt tcacgtgaac tgtcaaagta cccaagaaat taactattaa
6721 aattcgtaat ttagaaaaca atagtgattc cgaaaccgct ctactgtttt agacttttca
6781 ttgaggcact ggttatagtg gtggctttac agcattacca tattgtagac tttacagttt
6841 atcaccaagt ttctacccaa gaccacttca aggtgtttta tttttcattt tttttggcaa
6901 atttttttct acaaaatatt ctctgcttca acatacattt gttatgttga gaaacaggaa
6961 aattgaaaac aaaagaattg atccgtcccc cttttccttt gtcgatatca tgtaattagt
7021 tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt
7081 tagacaacct gaagtctagg tccctattta tttttttata gttatgttag tattaagaac
7141 gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat
7201 tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcaag
//