LOCUS LT727326 6592 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pMB-7, complete sequence.
ACCESSION LT727326
VERSION LT727326.1
KEYWORDS .
SOURCE Yeast expression vector pMB-7
ORGANISM Yeast expression vector pMB-7
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6592)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6592
/organism="Yeast expression vector pMB-7"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4216."
/db_xref="taxon:1945489"
CDS complement(625..1500)
/transl_table=11
/note="Salmonella hisG"
/protein_id="SJL88114.1"
/translation="MLDNTRLRIAIQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYLTLRRLDFG
GCRLSLATPVDEAWDGPAALDGKRIATSYPHLLKRYLDQKGVSFKSCLLNGSVEVAPR
AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMAQSKQELIDKLLTRIQ
GVIQARESKYIMMHAPSERLEEVIALLPGAERPTILPLAGEQQRVAMHMVSSETLFWE
TMDPRDNRVIDPWRN"
CDS complement(2089..2901)
/transl_table=11
/note="Candida URA3"
/protein_id="SJL88115.1"
/translation="MTVNTKTYSERAETHASPVAQRLFRLMELKKTNLCASIDVDTTK
EFLELIDKLGPYVCLIKTHIDIINDFSYESTIEPLLELSRKHQFMIFEDRKFADIGNT
VKKQYIGGVYKISSWADITNAHGVTGNGVVEGLKQGAKETTTNQEPRGLLMLAELSSV
GSLAYGEYSQKTVEIAKSDKEFVIGFIAQRDMGGQEEGFDWLIMTPGVGLDDKGDGLG
QQYRTVDEVVSTGTDIIIVGRGLFGKGRDPDIEGKRYRNAGWNAYLKKTGQL"
CDS complement(3370..4227)
/transl_table=11
/note="Salmonella hisG"
/protein_id="SJL88116.1"
/translation="MLDNTRLRIAIQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYLTLRRLDFG
GCRLSLATPVDEAWDGPAALDGKRIATSYPHLLKRYLDQKGVSFKSCLLNGSVEVAPR
AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMAQSKQELIDKLLTRIQ
GVIQARESKYIMMHAPSERLEEVIALLPGAERPTILPLAGEQQRVAMHMVSSETLFWE
TMDPLPCYP"
misc_feature complement(4376..4413)
/note="5' UTR of lacZ, incl. RBS"
regulatory complement(4414..4497)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(4589..4991)
/note="pMB1 ori"
CDS complement(5532..6392)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88117.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1676 a 1645 c 1534 g 1737 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
421 actctagagg atccctgttc cagtcaatca gggtattgaa gctcatggtc tttactccat
481 cacagggttc cgccttatcc ggcctacaga acccaaaata tcaacgcatt acgtaggcct
541 gataagcgca gcgccatcag gcgtcagatc actccatcat cttctcgatc ggcagtacca
601 gaatcgagct ggcgccaagc gctttcagtt tctccatgga tctattaccc tgttatccct
661 aggatccatg gtttcccaga acaacgtttc gctgctgacc atgtgcatcg ccacgcgctg
721 ttgctcgcct gccagcggca gaattgtcgg cctttcggcg cctggcagca gggcgataac
781 ctcttccagg cgttcacttg gcgcgtgcat catgatgtat ttcgattcgc gcgcctgaat
841 cacgccctga atacgggtca gcaatttatc gatcagctct tgcttgctct gtgccatctc
901 accgtcgcgc tgaatcagac aggctttaga gcggtagata acttcgactt cacgcaggcc
961 gttagcttca agcgtcgcgc cggtagagac caaatcgcag atagcgtcgg ccagccccgc
1021 gcgcggcgcg acttcgacag aaccatttaa cagacacgat ttaaaagaga cgcctttctg
1081 gtcgaggtag cgtttgagga ggtgcggata tgaggtagcg atacgtttac cgtccagcgc
1141 ggccgggccg tcccaggctt cgtcaaccgg tgttgccagc gataaacggc agccgccgaa
1201 gtcaagacgg cgcagggtta aatagcgtgg atcttcgccc tgtgcgcggc ggttgagtag
1261 ctcttcttcc agcacgtttt cgccgataat accgagatcg accacgccat ccattaccag
1321 acccggaatg tcatcatcac gcacgcgcag gatatcaatc ggcatgtttt ccgccatcgc
1381 aatcaggcgc tgagtgtgta aattaatttt tatgccgcag cgggccagca attctcgtga
1441 atcatcgctt aaacggcctg atttctgaat agctatgcgt aagcgggtgt tgtctaacat
1501 tctgcgttcc tctttatcct gtctgaaccg gtctgtatcg cgcgccaaaa aaaaagcccc
1561 cggaagatga tcttccgggg gctttctcat gcgttcatgc accactggaa gatccacagg
1621 acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg
1681 actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc
1741 atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg
1801 atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg
1861 gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg
1921 cgttagcaat ttaactgtga taaactaccg cattaaagct ctagaaggac cacctttgat
1981 tgtaaatagt aataattacc acccttattt aattatttat ttaacttatt tatttattta
2041 ttctacatat atacaaatct aataaagtga aaatctcccc cttcacattt ataattggcc
2101 agtctttttc aaataagcat tccaaccagc atttctatac cttttacctt caatatctgg
2161 atctcttcct ttaccaaaca atcctctacc aacaatgata atatcagttc cagtgctaac
2221 aacttcatca acagttctat attgttgtcc taatccatca cctttatcat ctaatccaac
2281 tccaggtgtc ataataagcc aatcaaatcc ttcttcttgg ccacccatat cacgttgggc
2341 aataaatcca ataacaaatt ccttatcgga tttagcaatt tcaacagttt tttgagaata
2401 ttctccatat gctaatgatc ccactgatga taattcagct aacatcaata accctcttgg
2461 ctcttggttg gtggtggttt ctttagctcc ctgttttaat ccttcaacca ctccattccc
2521 agtgacacca tgagcattgg taatatctgc ccaactacta attttataaa ctccaccaat
2581 atattgtttc tttacggtat taccaatatc agcaaatttt ctatcttcaa aaatcataaa
2641 ttgatgttta cgtgaaagtt ctaataatgg ttcaatagtg gattcatagg aaaaatcatt
2701 gattatatca atatgagtct tgattaagca tacataagga cctaatttat caattaattc
2761 aaggaattcc ttagtggtat caacgtcaat tgatgcacat aaattggttt tcttcagttc
2821 cattaatcga aataatcgct gtgctactgg tgaggcatga gtttctgctc tctcactata
2881 ggtcttagtg ttgactgtca tatcttttgt ttgctatttc tatttctatt tctttcttca
2941 actgtaaaag gggcggtata taaaccagtg tgtatggggt tgttgctcag gaattgatca
3001 atgtttaaaa gaatggttga aatcatcatt gaagaacaaa aaaaaagatt ttttctttca
3061 ttatgttatc tgtataatat gcaggaaata agattgcaaa aaaatagccg tttttttgga
3121 tactatcaaa caagaggaat gactaataca gaattagtgt tacgaatcaa tggcactaca
3181 gcaactttca acaaaataat ctacgttcaa aatataatat atactattaa ataaaatcaa
3241 ataataaaat aattttaaag aaagtagtac cagctctttt ttttgtttcc gtttatacca
3301 tccaaatcaa ttcctattag tgtaccagaa tcgagctggc gccaagcgct ttcagtttct
3361 ccatggatct tagggataac agggtaatgg atccatggtt tcccagaaca acgtttcgct
3421 gctgaccatg tgcatcgcca cgcgctgttg ctcgcctgcc agcggcagaa ttgtcggcct
3481 ttcggcgcct ggcagcaggg cgataacctc ttccaggcgt tcacttggcg cgtgcatcat
3541 gatgtatttc gattcgcgcg cctgaatcac gccctgaata cgggtcagca atttatcgat
3601 cagctcttgc ttgctctgtg ccatctcacc gtcgcgctga atcagacagg ctttagagcg
3661 gtagataact tcgacttcac gcaggccgtt agcttcaagc gtcgcgccgg tagagaccaa
3721 atcgcagata gcgtcggcca gccccgcgcg cggcgcgact tcgacagaac catttaacag
3781 acacgattta aaagagacgc ctttctggtc gaggtagcgt ttgaggaggt gcggatatga
3841 ggtagcgata cgtttaccgt ccagcgcggc cgggccgtcc caggcttcgt caaccggtgt
3901 tgccagcgat aaacggcagc cgccgaagtc aagacggcgc agggttaaat agcgtggatc
3961 ttcgccctgt gcgcggcggt tgagtagctc ttcttccagc acgttttcgc cgataatacc
4021 gagatcgacc acgccatcca ttaccagacc cggaatgtca tcatcacgca cgcgcaggat
4081 atcaatcggc atgttttccg ccatcgcaat caggcgctga gtgtgtaaat taatttttat
4141 gccgcagcgg gccagcaatt ctcgtgaatc atcgcttaaa cggcctgatt tctgaatagc
4201 tatgcgtaag cgggtgttgt ctaacattct gcgttcctct ttatcctgtc tgaaccggtc
4261 tgtatcgcgc gccaaaaaaa aagcccccgg aagatgatct tccgggggct ttctcatgcg
4321 ttcatgcacc actggaagat ctgggtaccg agctcgaatt cgtaatcatg gtcatagctg
4381 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
4441 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
4501 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
4561 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
4621 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
4681 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
4741 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
4801 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
4861 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
4921 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
4981 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
5041 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
5101 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
5161 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
5221 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
5281 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
5341 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
5401 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
5461 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
5521 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
5581 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
5641 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
5701 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
5761 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
5821 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
5881 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
5941 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
6001 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
6061 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
6121 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
6181 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
6241 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
6301 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
6361 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
6421 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
6481 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
6541 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//