LOCUS LT727322 6640 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pGAL1PSiST-1, complete sequence.
ACCESSION LT727322
VERSION LT727322.1
KEYWORDS .
SOURCE Yeast expression vector pGAL1PSiST-1
ORGANISM Yeast expression vector pGAL1PSiST-1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6640)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6640
/organism="Yeast expression vector pGAL1PSiST-1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4177."
/db_xref="taxon:1945483"
regulatory 113..383
/note="CYC1 terminator"
/regulatory_class="terminator"
regulatory complement(446..529)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(627..1035)
/note="pMB1 ori"
CDS complement(1576..2436)
/transl_table=11
/note="ampicillin"
/protein_id="SJL88101.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
CDS 3402..4214
/transl_table=11
/note="CaURA3"
/protein_id="SJL88102.1"
/translation="MTVNTKTYSERAETHASPVAQRLFRLMELKKTNLCASIDVDTTK
EFLELIDKLGPYVCLIKTHIDIINDFSYESTIEPLLELSRKHQFMIFEDRKFADIGNT
VKKQYIGGVYKISSWADITNAHGVTGNGVVEGLKQGAKETTTNQEPRGLLMLAELSSV
GSLAYGEYSQKTVEIAKSDKEFVIGFIAQRDMGGQEEGFDWLIMTPGVGLDDKGDGLG
QQYRTVDEVVSTGTDIIIVGRGLFGKGRDPDIEGKRYRNAGWNAYLKKTGQL"
regulatory 4593..5805
/note="CaGAL1 promoter"
/regulatory_class="promoter"
CDS 5873..6436
/transl_table=11
/note="hIFNbeta"
/protein_id="SJL88103.1"
/translation="MTNKCLLQIALLLCFSTTALSMSYNLLGFLQRSSNFQCQKLLWQ
LNGRLEYCLKDRMNFDIPEEIKQLQQFQKEDAALTIYEMLQNIFAIFRQDSSSTGWNE
TIVENLLANVYHQINHLKTVLEEKLEKEDFTRGKLMSSLHLKRYYGRILHYLKAKEYS
HCAWTIVRVEILRNFYFINRLTGYLRN"
misc_feature 6437..6636
/note="3' UTR of hIFNbeta"
BASE COUNT 1935 a 1342 c 1466 g 1897 t
ORIGIN
1 gaggcctagc ggccgcctag aggatccccg ggcgctaggc ggccgctagg cctttttggc
61 caagctcgaa tttcgaggaa ttcgagctcg gtacccgggg gatcgatccg tccccctttt
121 cctttgtcga tatcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat
181 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
241 ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt
301 acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac
361 gctcgaaggc tttaatttgc aagctagctt ggcgtaatca tggtcatagc tgtttcctgt
421 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
481 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
541 tttccagtcg ggaaacctgt cgtgccagag atctctgcat taatgaatcg gccaacgcgc
601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
661 ctcggtcgtt cggctgcggc gagcggtatc agatcgatct cactcaaagg cggtaatacg
721 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
781 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
841 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
901 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
961 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
1021 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
1081 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
1141 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
1201 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
1261 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
1321 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
1381 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
1441 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
1501 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
1561 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
1621 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
1681 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
1741 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
1801 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
1861 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
1921 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
1981 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
2041 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
2101 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
2161 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
2221 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
2281 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
2341 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
2401 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
2461 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
2521 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
2581 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
2641 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
2701 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
2761 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
2821 tatcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
2881 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
2941 ccggccacgg ggcctgccac catacccacg ccgaaacaag cactaatagg aattgatttg
3001 gatggtataa acggaaacaa aaaaaagagc tggtactact ttctttaaaa ttattttatt
3061 atttgatttt atttaatagt atatattata ttttgaacgt agattatttt gttgaaagtt
3121 gctgtagtgc cattgattcg taacactaat tctgtattag tcattcctct tgtttgatag
3181 tatccaaaaa aacggctatt tttttgcaat cttatttcct gcatattata cagataacat
3241 aatgaaagaa aaaatctttt tttttgttct tcaatgatga tttcaaccat tcttttaaac
3301 attgatcaat tcctgagcaa caaccccata cacactggtt tatataccgc cccttttaca
3361 gttgaagaaa gaaatagaaa tagaaatagc aaacaaaaga tatgacagtc aacactaaga
3421 cctatagtga gagagcagaa actcatgcct caccagtagc acagcgatta tttcgattaa
3481 tggaactgaa gaaaaccaat ttatgtgcat caattgacgt tgataccact aaggagttcc
3541 tcgagttaat tgataaatta ggtccttatg tatgcttaat caagactcat attgatataa
3601 tcaatgattt ttcctatgaa tccactattg aaccattatt agaactttca cgtaaacatc
3661 aatttatgat ttttgaagat agaaaatttg ctgatattgg taataccgta aagaaacaat
3721 atattggtgg agtttataaa attagtagtt gggcagatat taccaatgct catggtgtca
3781 ctgggaatgg agtggttgaa ggattaaaac agggagctaa agaaaccacc accaaccaag
3841 agccaagagg gttattgatg ttagctgaat tatcatcagt gggatcatta gcatatggag
3901 aatattctca aaaaactgtt gaaattgcta aatccgataa ggaatttgtt attggattta
3961 ttgcccaacg tgatatgggt ggccaagaag aaggatttga ttggcttatt atgacacctg
4021 gagttggatt agatgataaa ggtgatggat taggacaaca atatagaact gttgatgaag
4081 ttgttagcac tggaactgat attatcattg ttggtagagg attgtttggt aaaggaagag
4141 atccagatat tgaaggtaaa aggtatagaa atgctggttg gaatgcttat ttgaaaaaga
4201 ctggccaatt ataaatgtga agggggagat tttcacttta ttagatttgt atatatgtag
4261 aataaataaa taaataagtt aaataaataa ttaaataagg gtggtaatta ttactattta
4321 caatcaaagg tggtccttct agctgtaatc cgggcagcgc aacggaacat tcatcagtgt
4381 aaaaatggaa tcaataaagc cctgcgctca tgagcccgaa gtggcgagcc cgatcttccc
4441 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg cagcgcgcag
4501 ggtcagcctg aatacgcgtt taatgaccag cacagtcgtg atggcaaggt cagaatagcc
4561 caagtcggcc gaggggcctg tacagtgagg gaagatctga tattgacgaa gaggaaccaa
4621 tgtaacgtta cactgaagaa aacacacaat aaacgggaag aaacggtgta aaagtgtgaa
4681 aataattttt gaatatcatt tcccttggtt taattccaaa cgaaacgtgt tttttttaga
4741 gaatgggaat tcttattgga tgtctagatt gtttgtttac tccagactgt gcacaaaaac
4801 gtttggatgg atgatcagaa gatattttta ggcttagctc taaatataag aaatgatgct
4861 tgaaaaacca gacagaaatt gagtttcaaa aattggtaat gtgaggtatt agtcaactaa
4921 ccaaataaca atgcaaaccg gttgatacat ttcattttga aaataatgaa actggaattg
4981 gatgaccagc acacaaacac ataaagtaat tatgggaatt agaagcgaac atagaggagt
5041 acttggccac gaacagaata caagtgggaa cactattttc tccattgttt tagttctgtt
5101 tttttgtcag cctagttttg tgctatgtgt aaaaaatatt gccaagaaaa aaagcttgtt
5161 ttgtggccag tgtccgaaaa aaattttggg gaatcttcgg attaatttat gttttcattc
5221 catcggggaa agtggggggg aaaaaatttt aagcagttca caaaaccttc caaaaaatat
5281 atggacaaag atgattgtat tttcccgaca ccaaaatcat aattaattat gagaaagtta
5341 aatgtaacgt tacaatttat gtttatttga aggtgaaaag cgatttatga tttttccgaa
5401 atgaaaattt tttttaggtt tatttttttt gtcgggcaaa gaaaaactga acaaggatta
5461 ttaaaatttt tggtgtttgt ttgtgtctgg agaattcatt cctctctcat cttcacacaa
5521 tgtttagaca tctgacacga ttcatgatag ttcggtttcc ggggttggtg tttagttttc
5581 gtttttcttt ttttttggaa agaatgtttt agctcattgg ttttctttct tcattcaata
5641 gttttgaaag aatttgccca cttgttatta caatcatata aaattaaact ttgatataaa
5701 atagagtttg aaagtttccc agatcctttt tgatttcttt gtaaattttt ttttctccca
5761 catatacaca catacaaacc gatttttata agaaagagtt ataccctgca gctcgacctc
5821 gactgtttaa acctgcaggc atgcaagctt ggccaaaaag gcctcgagga acatgaccaa
5881 caagtgtctc ctccaaattg ctctcctgtt gtgcttctcc actacagctc tttccatgag
5941 ctacaacttg cttggattcc tacaaagaag cagcaatttt cagtgtcaga agctcctgtg
6001 gcaattgaat gggaggcttg aatactgcct caaggacagg atgaactttg acatccctga
6061 ggagattaag cagctgcagc agttccagaa ggaggacgcc gcattgacca tctatgagat
6121 gctccagaac atctttgcta ttttcagaca agattcatct agcactggct ggaatgagac
6181 tattgttgag aacctcctgg ctaatgtcta tcatcagata aaccatctga agacagtcct
6241 ggaagaaaaa ctggagaaag aagatttcac caggggaaaa ctcatgagca gtctgcacct
6301 gaaaagatat tatgggagga ttctgcatta cctgaaggcc aaggagtaca gtcactgtgc
6361 ctggaccata gtcagagtgg aaatcctaag gaacttttac ttcattaaca gacttacagg
6421 ttacctccga aactgaagat ctcctagcct gtgcctctgg gactggacaa ttgcttcaag
6481 cattcttcaa ccagcagatg ctgtttaagt gactgatggc taatgtactg catatgaaag
6541 gacactagaa gattttgaaa tttttattaa attatgagtt atttttattt atttaaattt
6601 tattttggaa aataaattat ttttggtgca aaagtccctc
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