LOCUS LT727321 8408 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-mCASP-2, complete sequence.
ACCESSION LT727321
VERSION LT727321.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-mCASP-2
ORGANISM Mammalian expression vector pCAGGS-mCASP-2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8408)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8408
/organism="Mammalian expression vector pCAGGS-mCASP-2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4176."
/db_xref="taxon:1944881"
misc_feature 88..170
/note="5' UTR of mCASP-2"
CDS 171..1529
/transl_table=11
/note="mCASP-2"
/protein_id="SJL88099.1"
/translation="MAAPSGRSQSSLHRKGLMAADRRSRILAVCGMHPDHQETLKKNR
VVLAKQLLLSELLEHLLEKDIITLEMRELIQAKGGSFSQNVELLNLLPKRGPQAFDAF
CEALRETRQGHLEDLLLTTLSDIQHVLPPLSCDYDTSLPFSVCESCPPHKQLRLSTDA
TEHSLDNGDGPPCLLVKPCTPEFYQAHYQLAYRLQSQPRGLALVLSNVHFTGEKDLEF
RSGGDVDHTTLVTLFKLLGYNVHVLHDQTAQEMQEKLQNFAQLPAHRVTDSCVVALLS
HGVEGGIYGVDGKLLQLQEVFRLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDG
KNHTQSPGCEESDAGKEELMKMRLPTRSDMICGYACLKGNAAMRNTKRGSWYIEALTQ
VFSERACDMHVADMLVKVNALIKEREGYAPGTEFHRCKEMSEYCSTLCQQLYLFPGYP
PT"
misc_feature 1530..3548
/note="3' UTR of mCASP-2"
regulatory 3698..3789
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 3790..4146
/note="3' flanking region of rabbit beta-globin"
regulatory complement(4224..4306)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 4448..4523
/note="SV40 ori"
regulatory 4554..4628
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4554..4628)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(4743..5144)
/note="pMB1 ori"
CDS complement(5685..6545)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88100.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 6681..7058
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 7064..8405
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 7433..8357
/note="intron AG promoter"
BASE COUNT 1808 a 2124 c 2188 g 2288 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 ggatccccgg gaattcggca cgagggctgg gggagggatg cgggggctgg cgcggcccag
121 cgtttgtttg ttttgtgggt ggtgcgtgtg cagcccggga aaagctggaa atggcggcgc
181 cgagcgggag gtcgcagtcc tccctgcaca ggaaggggct gatggcggct gacaggagga
241 gcaggatttt ggcagtgtgt ggaatgcatc ctgaccacca ggaaacactg aaaaagaatc
301 gagtggtgct ggccaagcag ctgctgctga gcgagctgtt agaacacctc ctagagaagg
361 acattatcac tttggaaatg agggagctca tccaggccaa agggggcagt ttcagccaga
421 atgtggaact cctcaacctg ctgccaaaga gaggacccca ggcttttgat gccttctgtg
481 aagccctgcg ggagaccagg cagggtcact tggaagactt actgctcaca accctctcag
541 atattcagca cgtactccca ccgttgagct gtgactatga cacaagtctc cctttctcgg
601 tgtgtgagtc ctgccctcct cacaagcagc tccgcctatc cacagatgct acggaacact
661 ccttagataa tggtgatggt cctccctgtc ttctggtgaa gccatgcact cctgagtttt
721 accaggcaca ctaccagctg gcctataggt tgcaatctca gccccgtggc ttggcactgg
781 tgctgagcaa tgtgcacttc actggagaga aagacctgga attccgctct ggaggggatg
841 tggaccacac tactctagtc accctcttca agcttttggg ctacaatgtc catgtgctac
901 atgaccagac cgcacaggaa atgcaagaga aacttcagaa ttttgcacag ttacctgcac
961 accgggtcac agactcctgc gtagtggcac tcctctcaca tggtgtggaa ggtggcatct
1021 atggtgtaga tggcaaactg cttcagctcc aagaggtttt tcgacttttt gacaatgcta
1081 actgtccaag tctacagaac aagccaaaaa tgttcttcat ccaagcatgt cgtggagatg
1141 agacagatag aggtgtcgac cagcaagatg gaaagaacca cacacaatcc cctggatgtg
1201 aggagagtga tgctggcaaa gaggagttga tgaagatgag actgcctact cgctcagaca
1261 tgatatgtgg ctatgcttgc cttaaaggta atgctgccat gcggaacacc aaacggggtt
1321 cctggtacat tgaggccctc actcaggtgt tctctgaaag agcttgtgac atgcacgtgg
1381 ccgacatgct tgttaaggtg aatgccctta tcaaggagcg tgaaggctat gcccctggca
1441 cagaattcca ccgatgcaag gagatgtctg agtactgtag tactctgtgc cagcaactct
1501 acctgttccc aggctaccca cccacgtgat gccgcctgct attcctgctg ttggaggcca
1561 ctggaccact gggggcacaa tggagacttc tcttcagaat ggtttttgtt ctgtctaccc
1621 tctcagggat atgagattct cccaggcttg tttcctgtca gccatctctg tctttgggta
1681 tgaaacataa ggatggctcc tccggtgtcg tgttctctac ctatagagcc agctctgaat
1741 ggatgtgtta ccagaagcat tttagctaca gcctagaaaa tgacattgtg aacacagtat
1801 tattgtggga agagggcatt tggatttctc aatgtttgtg atatttttgt tcccaaggca
1861 tcttaggagt acttggatca tagctttttt tttttttcct aaatcagtta aggagtctca
1921 gagatcatct cctttttttt ccatatctac aacctcattt ttcccacagt ggagatttgg
1981 aagatgtccc aatttaatgt aggtgttttc atctgtcatg aagggacaga tgagatccta
2041 ctacttgcga agtttctatg cataccttta agttcaggcc ctaggtgaag gacagtccct
2101 cagcctttcc attggttcct ttgtgttcag tgcacccagc ctttgaacag agcctagggt
2161 ctgtatgcca tgacactgga agtcatagaa atttccctgg tcatgctttg tttgaactgt
2221 cactgaatga accttatcgg gcataactac atgaaaatgc agtgacagct gagtgtgctg
2281 tgtctcacac tatcacccgt catcaggatg tctctccttc cttactgtgg cttctgcatg
2341 cacttacact gtacttgacg gctggcctcc agggtctctc ttgctttgta ctggttcccc
2401 tctttacctt caccattcgc tgcttctgcc aagtctgtga agccgtcctt tgtaggatgt
2461 ttcttgccac ttacgctgta ctgtagttgc ttattctttc tgccttctgc ttcagcgtga
2521 ggcttctttg gttttctgtg gcagcgtctc ccttctcatt gtttctctgt gttttagtgg
2581 ggatagtacc atatgtgata taacctagaa gcacttgtct ctgctcttat gaaacttgct
2641 tattcttgaa aaccttctgc atttccattt tttcctctct tccaatttat tctccatgta
2701 acagagtagt ttggttttta aaatatctgg tgatgtcatt ctcttgctta gaacactagc
2761 ttcctgttac gcttcatcta aaatgcaaat tcttacaccc agcttacgag atctggctca
2821 taccttccct ttggatctca ttaaatggtg atttctcact atgctccagc ccctcttagg
2881 tcctcatctc cgtcttgcag gtgttctgaa ctctcctttg gctagtctct gatttttgag
2941 tctggcggag gcctcttgac catgctgccc atgctgtcta ctgtgcctcc ttatgagggc
3001 atcatgttgg tctctgttgt gcttactgca ggctgtaatg gcacttttgc ttgtttcact
3061 tgttccctct gaggctgaat gctccaagag agtgggaact gtgcttctta cttactgata
3121 tccagtaact ggcacttact aggtcttcat gaatgtttcc tgagtaaagg aaggagacca
3181 gcagctaacc ttagttagag cctacctttt gcagtttcta aattgctatt atagtgtaca
3241 gttcaattag tatatgggtt tttttttcca ggtgttttat ttttatccac tgttttgttg
3301 ttgttttttt atattttcta catatcacgt gtgagacaac ttctttcaca tctccatagt
3361 gcccagcaaa tttgaggcct atggtagttg aggtgctcaa ctaatgtttg ttgtatgaac
3421 caagtggttt gaagacttgc tgccaaattc tgccttttgg gtcagtatag gatgcataag
3481 tggtagaatc ttcacacttc ccactgccaa gattttgtat tgccatcagg tgccaaataa
3541 atgttgatct cgtgccgaat tcgatctctt tttttgggtt tggtggggta tcttcatcat
3601 cgaatagata gttatataca tcatccattg tagtggtatt aaacatccct gtagtgattc
3661 caaacgcgcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
3721 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
3781 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
3841 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
3901 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
3961 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
4021 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
4081 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
4141 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
4201 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
4261 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
4321 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
4381 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
4441 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
4501 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
4561 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
4621 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
4681 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
4741 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
4801 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga
4861 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
4921 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
4981 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
5041 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
5101 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
5161 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
5221 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
5281 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
5341 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
5401 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
5461 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
5521 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
5581 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
5641 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
5701 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
5761 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
5821 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
5881 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
5941 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
6001 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
6061 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
6121 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
6181 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
6241 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
6301 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
6361 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
6421 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
6481 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
6541 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
6601 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
6661 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
6721 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
6781 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
6841 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
6901 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
6961 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
7021 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
7081 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
7141 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc
7201 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag
7261 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
7321 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc
7381 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
7441 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
7501 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
7561 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
7621 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
7681 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
7741 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
7801 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
7861 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
7921 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg
7981 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
8041 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
8101 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
8161 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
8221 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
8281 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
8341 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
8401 gcaaagaa
//