LOCUS LT727320 6308 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-mCASP-1, complete sequence.
ACCESSION LT727320
VERSION LT727320.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-mCASP-1
ORGANISM Mammalian expression vector pCAGGS-mCASP-1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6308)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6308
/organism="Mammalian expression vector pCAGGS-mCASP-1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4175."
/db_xref="taxon:1944874"
misc_feature 42..118
/note="5' UTR of mCASP-1"
CDS 119..1327
/transl_table=11
/note="mCASP-1"
/protein_id="SJL88097.1"
/translation="MADKILRAKRKQFINSVSIGTINGLLDELLEKRVLNQEEMDKIK
LANITAMDKARDLCDHVSKKGPQASQIFITYICNEDCYLAGILELQSAPSAETFVATE
DSKGGHPSSSETKEEQNKEDGTFPGLTGTLKFCPLEKAQKLWKENPSEIYPIMNTTTR
TRLALIICNTEFQHLSPRVGAQVDLREMKLLLEDLGYTVKVKENLTALEMVKEVKEFA
ACPEHKTSDSTFLVFMSHGIQEGICGTTYSNEVSDILKVDTIFQMMNTLKCPSLKDKP
KVIIIQACRGEKQGVVLLKDSVRDSEEDFLTDAIFEDDGIKKAHIEKDFIAFCSSTPD
NVSWRHPVRGSLFIESLIKHMKEYAWSCDLEDIFRKVRFSFEQPEFRLQMPTADRVTL
TKRFYLFPGH"
misc_feature 1328..1438
/note="3' UTR of mCASP-1"
regulatory 1598..1689
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1690..2046
/note="3' FR of beta-globin"
regulatory complement(2124..2206)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2348..2423
/note="SV40 ori"
regulatory 2454..2528
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2454..2528)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2643..3044)
/note="pMB1 ori"
CDS complement(3585..4445)
/transl_table=11
/note="ampicillin"
/protein_id="SJL88098.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4581..4958
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4964..6305
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5333..6257
/note="intron AG promoter"
BASE COUNT 1495 a 1573 c 1673 g 1567 t
ORIGIN
1 ttcctcgaag cttggccaaa acggccgagc tcgaattcgt ccggccgagc tcgaattcgt
61 caggccaaca caatatgctt tcagtttcag tagctctgcg gtgtagaaaa gaaacgccat
121 ggctgacaag atcctgaggg caaagaggaa gcaatttatc aactcagtga gtatagggac
181 aataaatgga ttgttggatg aacttttaga gaagagagtg ctgaatcagg aagaaatgga
241 taaaataaaa cttgcaaaca ttactgctat ggacaaggca cgggacctat gtgatcatgt
301 ctctaaaaaa gggccccagg caagccaaat ctttatcact tacatttgta atgaagactg
361 ctacctggca ggaattctgg agcttcaatc agctccatca gctgaaacat ttgttgctac
421 agaagattct aaaggaggac atccttcatc ctcagaaaca aaggaagaac agaacaaaga
481 agatggcaca tttccaggac tgactgggac cctcaagttt tgccctttag aaaaagccca
541 gaagttatgg aaagaaaatc cttcagagat ttatccaata atgaatacaa ccactcgtac
601 acgtcttgcc ctcattatct gcaacacaga gtttcaacat ctttctccga gggttggagc
661 tcaagttgac ctcagagaaa tgaagttgct gctggaggat ctggggtata ccgtgaaagt
721 gaaagaaaat ctcacagctc tggagatggt gaaagaggtg aaagaatttg ctgcctgccc
781 agagcacaag acttctgaca gtactttcct tgtattcatg tctcatggta tccaggaggg
841 aatatgtggg accacatact ctaatgaagt ttcagatatt ttaaaggttg acacaatctt
901 tcagatgatg aacactttga agtgcccaag cttgaaagac aagcccaagg tgatcattat
961 tcaggcatgc cgtggagaga aacaaggagt ggtgttgtta aaagattcag taagagactc
1021 tgaagaggat ttcttaacgg atgcaatttt tgaagatgat ggcattaaga aggcccatat
1081 agagaaagat tttattgctt tctgctcttc aacaccagat aatgtgtctt ggagacatcc
1141 tgtcaggggc tcacttttca ttgagtcact catcaaacac atgaaagaat atgcctggtc
1201 ttgtgacttg gaggacattt tcagaaaggt tcgattttca tttgaacaac cagaatttag
1261 gctacagatg cccactgctg atagggtgac cctgacaaaa cgtttctacc tcttcccggg
1321 acattaaacg aagaatccag ttcattctta tgtacctatg ctgagaatcg tgccaataag
1381 aagccaatac ttccttagat gatgcaataa atattaaaat aaaacaaaac agaaggctaa
1441 aaaaaaaaaa aaaaaagctc gcccggggat cctctaggcg gccgctaggc ctttttggcc
1501 aagctcgaat ttcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
1561 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
1621 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
1681 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
1741 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
1801 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
1861 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
1921 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
1981 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
2041 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
2101 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
2161 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
2221 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
2281 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
2341 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
2401 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
2461 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
2521 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
2581 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
2641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
2701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga
2761 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
2821 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
2881 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
2941 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
3001 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
3061 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
3121 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
3181 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
3241 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
3301 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
3361 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
3421 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
3481 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
3541 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
3601 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
3661 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
3721 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
3781 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
3841 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
3901 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
3961 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
4021 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
4081 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
4141 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
4201 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
4261 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
4321 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
4381 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
4441 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
4501 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
4561 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
4621 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
4681 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
4741 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
4801 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
4861 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
4921 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
4981 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
5041 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc
5101 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag
5161 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
5221 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc
5281 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
5341 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
5401 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
5461 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
5521 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
5581 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
5641 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
5701 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
5761 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
5821 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg
5881 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
5941 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
6001 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
6061 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
6121 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
6181 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
6241 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
6301 gcaaagaa
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