LOCUS LT727319 8321 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-prepro-TSE, complete sequence.
ACCESSION LT727319
VERSION LT727319.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-prepro-TSE
ORGANISM Mammalian expression vector pCAGGS-prepro-TSE
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8321)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8321
/organism="Mammalian expression vector pCAGGS-prepro-TSE"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4172."
/db_xref="taxon:1944894"
misc_difference 428..670
/note="ppMF"
CDS 698..>2629
/transl_table=11
/note="mature TS"
/protein_id="SJL88095.1"
/translation="MLAPGSSRVELFKRKNSTVPFEDKAGKVTERVVHSFRLPALVNV
DGVMVAIADARYDTSNDNSLIDTVAKYSVDDGETWETQIAIKNSRVSSVSRVVDPTVI
VKGNKLYVLVGSYYSSRSYWSSHGDARDWDILLAVGEVTKSIAGGKITASIKWGSPVS
LKKFFPAEMEGMHTNQFLGGAGVAIVASNGNLVYPVQVTNKRKQVFSKIFYSEDDGKT
WKFGKGRSDFGCSEPVALEWEGKLIINTRVDWKRRLVYESSDMGNTWVEAVGTLSRVW
GPSPKSDHPGSQSSFTAVTIEGMRVMLFTHPLNFKGRWLRDRLNLWLTDNQRIYNVGQ
VSIGDENSAYSSVLYKDDKLYCLHEINTDEVYSLVFARLVGELRIIKSVLRSWKNWDS
HLSSICTPADPAASSSESGCGPAVTTVGLVGFLSGNASQNVWEDAYRCVNASTANAER
VRNGLKFAGVGGGALWPVSQQGQNQRYRFANHAFTLVASVTIHEAPRAASPLLGASLD
SSGGKKLLGLSYDEKHQWQPIYGSTPVTPTGSWETGKRYHVVLTVANKIGSVYIDGEL
LEGSGQTVVPDGRTPDISHFYVGGYGRSDMPTISHVTVNNVLLYNRQLNTEEIRTLFL
SQDLIGTEAHMDSSSDTKEL"
misc_feature 2630..2668
/note="E-tag"
regulatory 3611..3702
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 3703..4059
/note="3' flanking region of rabbit beta-globin"
regulatory complement(4137..4219)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 4361..4436
/note="SV40 ori"
regulatory 4467..4541
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4467..4541)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(4656..5057)
/note="pMB1 ori"
CDS complement(5598..6458)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88096.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 6591..6971
/note="hCMV enhancer"
/regulatory_class="promoter"
regulatory 6977..8318
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 7346..8270
/note="intron AG promoter"
BASE COUNT 1841 a 2086 c 2322 g 2072 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 gtaatgctta gcgcagtctc tctatcgctt ctgaaccccg gtgcacctgt gccgaaacgc
121 aaatggggaa acacccgctt tttggatgat tatgcattgt ctccacattg tatgcttcca
181 agattctggt gggaatactg ctgatagcct aacgttcatg atcaaaattt aactgttcta
241 acccctactt gacagcaata tataaacaga aggaagctgc cctgtcttaa accttttttt
301 ttatcatcat tattagctta ctttcataat tgcgactggt tccaattgac aagcttttga
361 ttttaacgac ttttaacgac aacttgagaa gatcaaaaaa caactaatta ttcgaaggat
421 ccaaacgatg agatttcctt caatttttac tgcagtttta ttcgcagcat cctccgcatt
481 agctgctcca gtcaacacta caacagaaga tgaaacggca caaattccgg ctgaagctgt
541 catcggttac tcagatttag aaggggattt cgatgttgct gttttgccat tttccaacag
601 cacaaataac gggttattgt ttataaatac tactattgcc agcattgctg ctaaagaaga
661 aggggtatct ctcgagaaaa gagaggctga agcttacgtg ctggcacccg gatcgagccg
721 agttgagctg tttaagcgta agaattcgac ggtgccgttt gaagacaagg ccggcaaagt
781 caccgagcgg gttgtccact cgttccgcct ccccgccctt gttaatgtgg acggggtgat
841 ggttgccatc gcggacgctc gctacgacac atccaatgac aactccctca ttgatacggt
901 ggcgaagtac agcgtggacg atggggagac gtgggagacc caaattgcca tcaagaacag
961 ccgtgtatcg tctgtttctc gtgtggtgga tcccaccgtg attgtgaagg gcaacaagct
1021 ttacgtcctg gttggaagct actatagttc gagaagctac tggtcgtcgc atggtgatgc
1081 gagagactgg gatattctgc ttgccgttgg tgaggtcacg aagtccattg cgggcggcaa
1141 gataactgcg agtatcaaat gggggagccc cgtgtcactg aagaagtttt ttccggcaga
1201 aatggaaggc atgcacacaa atcaatttct tggcggcgcg ggtgttgcca ttgtggcgtc
1261 caacgggaat cttgtgtacc ctgtgcaggt tacgaacaaa aggaagcaag ttttctccaa
1321 gatcttctac tcggaagatg atgggaagac gtggaagttt gggaagggta ggagcgattt
1381 tggctgctct gaacctgtgg cccttgagtg ggaggggaag ctcatcataa acacccgagt
1441 tgactggaaa cgccgtctgg tgtacgagtc cagtgacatg gggaatacgt gggtggaggc
1501 tgtcggcacg ctctcgcgtg tgtggggccc ctcaccaaaa tcggaccatc ccggcagtca
1561 gagcagcttc actgccgtga ccatcgaagg aatgcgtgtg atgctcttca cacacccgct
1621 gaattttaag ggaaggtggc tgcgcgaccg actgaacctc tggctgacgg ataaccagcg
1681 catttataac gttgggcaag tatccattgg tgatgaaaat tccgcctaca gctccgtcct
1741 gtacaaggat gataagctgt actgtttgca tgagatcaac acggacgagg tgtacagcct
1801 tgtttttgca cgcctggttg gcgagctacg gatcattaaa tcagtgctgc gatcctggaa
1861 gaattgggac agccacctgt ccagcatttg cacccctgct gatccagccg cttcgtcgtc
1921 agagagtggt tgtggtcccg ctgtcaccac ggttggtctt gttggctttt tgtccggcaa
1981 tgcctcccaa aacgtatggg aggatgcgta ccgctgcgtc aacgcaagca cggcaaatgc
2041 ggagagggtt cggaacggtt tgaagtttgc gggggttggc ggaggggcgc tttggccggt
2101 gagccagcag gggcagaatc agcggtatcg ttttgcaaac cacgcgttca cgctggtggc
2161 gtcggtgacg attcacgagg ctccgagggc cgcgagtcct ttgctgggtg cgagcctgga
2221 ctcttctggt ggcaaaaaac tcctggggct ctcgtacgac gagaagcacc agtggcagcc
2281 aatatacgga tcaacgccgg tgacgccgac gggatcgtgg gagacgggta agaggtacca
2341 cgtggttctt acggtggcga ataaaattgg ctccgtgtac attgatggag aacttctgga
2401 gggttcagga cagaccgttg tgccagacgg gaggacgcct gacatctccc acttctacgt
2461 tggcgggtat ggaaggagtg atatgccaac cataagccac gtgacggtga ataatgttct
2521 tctttacaac cgacagctga ataccgagga gatcaggacc ttgttcttga gccaggacct
2581 gattggcacg gaagcacaca tggacagcag cagcgacacg aaggaattgg gtgcgccggt
2641 gccgtatccg gatccgctgg aaccgcgtgc cgcatagact gttgaaagtt gtttagcaaa
2701 acctcataca gaaaattcat ttactaacgt ctggaaagac gacaaaactt tagatcgtta
2761 cgctaactat gagggctgtc tgtggaatgc tacaggcgtt gtggtttgta ctggtgacga
2821 aactcagtgt tacggtacat gggttcctat tgggcttgct atccctgaaa atgagggtgg
2881 tggctctgag ggtggcggtt ctgagggtgg cggttctgag ggtggcggta ctaaacctcc
2941 tgagtacggt gatacaccta ttccgggcta tacttatatc aaccctctcg acggcactta
3001 tccgcctggt actgagcaaa accccgctaa tcctaatcct tctcttgagg agtctcagcc
3061 tcttaatact ttcatgtttc agaataatag gttccgaaat aggcagggtg cattaactgt
3121 ttatacgggc actgttactc aaggcactga ccccgttaaa acttattacc agtacactcc
3181 tgtatcatca aaagccatgt atgacgctta ctggaacggt aaattcagag actgcgcttt
3241 ccattctggc tttaatgagg atccattcgt ttgtgaatat caaggccaat cgtctgacct
3301 gcctcaacct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg gcggctctga
3361 gggtggcggc tctgagggtg gcggttctga gggtggcggc tctgagggtg gcggttccgg
3421 tggcggctcc ggttccggtg attttgatta tgaaaaaatg gcaaacgcta ataagggggc
3481 tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca aacttgattc
3541 tgtcgctact gattacggtg ctgctatcgg gccgcgaatt accaatgccc tggctcacaa
3601 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
3661 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
3721 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
3781 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
3841 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
3901 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
3961 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
4021 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
4081 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
4141 acaacatacg agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact
4201 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
4261 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
4321 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
4381 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
4441 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat
4501 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
4561 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg
4621 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
4681 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
4741 acggttatcc acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
4801 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
4861 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
4921 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
4981 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
5041 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
5101 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
5161 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
5221 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
5281 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
5341 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
5401 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
5461 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
5521 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
5581 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
5641 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
5701 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
5761 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
5821 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
5881 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
5941 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
6001 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
6061 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
6121 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
6181 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
6241 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
6301 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
6361 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
6421 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
6481 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
6541 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg
6601 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
6661 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
6721 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
6781 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
6841 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
6901 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
6961 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
7021 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
7081 gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
7141 gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
7201 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt
7261 tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
7321 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
7381 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
7441 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt
7501 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg
7561 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc
7621 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
7681 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc
7741 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg
7801 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
7861 tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc
7921 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
7981 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
8041 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
8101 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
8161 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
8221 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
8281 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a
//