LOCUS LT727318 6757 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-TS, complete sequence.
ACCESSION LT727318
VERSION LT727318.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-TS
ORGANISM Mammalian expression vector pCAGGS-TS
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6757)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6757
/organism="Mammalian expression vector pCAGGS-TS"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4167."
/db_xref="taxon:1944899"
CDS 73..2004
/transl_table=11
/note="mature TS"
/protein_id="SJL88093.1"
/translation="MLAPGSSRVELFKRKNSTVPFEDKAGKVTERVVHSFRLPALVNV
DGVMVAIADARYDTSNDNSLIDTVAKYSVDDGETWETQIAIKNSRVSSVSRVVDPTVI
VKGNKLYVLVGSYYSSRSYWSSHGDARDWDILLAVGEVTKSIAGGKITASIKWGSPVS
LKKFFPAEMEGMHTNQFLGGAGVAIVASNGNLVYPVQVTNKRKQVFSKIFYSEDDGKT
WKFGKGRSDFGCSEPVALEWEGKLIINTRVDWKRRLVYESSDMGNTWVEAVGTLSRVW
GPSPKSDHPGSQSSFTAVTIEGMRVMLFTHPLNFKGRWLRDRLNLWLTDNQRIYNVGQ
VSIGDENSAYSSVLYKDDKLYCLHEINTDEVYSLVFARLVGELRIIKSVLRSWKNWDS
HLSSICTPADPAASSSESGCGPAVTTVGLVGFLSGNASQNVWEDAYRCVNASTANAER
VRNGLKFAGVGGGALWPVSQQGQNQRYRFANHAFTLVASVTIHEAPRAASPLLGASLD
SSGGKKLLGLSYDEKHQWQPIYGSTPVTPTGSWETGKRYHVVLTVANKIGSVYIDGEL
LEGSGQTVVPDGRTPDISHFYVGGYGRSDMPTISHVTVNNVLLYNRQLNTEEIRTLFL
SQDLIGTEAHMDSSSDTSA"
regulatory 2047..2138
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 2139..2495
/note="3' flanking region of rabbit beta-globin"
regulatory complement(2573..2655)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2797..2872
/note="SV40 ori"
regulatory 2903..2977
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2903..2977)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3092..3493)
/note="pMB1 ori"
CDS complement(4034..4894)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88094.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5030..5407
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5413..6754
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5782..6706
/note="intron AG promoter"
BASE COUNT 1441 a 1744 c 1947 g 1625 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 ggccatggcc atatgctggc acccggatcg agccgagttg agctgtttaa gcgtaagaat
121 tcgacggtgc cgtttgaaga caaggccggc aaagtcaccg agcgggttgt ccactcgttc
181 cgcctccccg cccttgttaa tgtggacggg gtgatggttg ccatcgcgga cgctcgctac
241 gacacatcca atgacaactc cctcattgat acggtggcga agtacagcgt ggacgatggg
301 gagacgtggg agacccaaat tgccatcaag aacagccgtg tatcgtctgt ttctcgtgtg
361 gtggatccca ccgtgattgt gaagggcaac aagctttacg tcctggttgg aagctactat
421 agttcgagaa gctactggtc gtcgcatggt gatgcgagag actgggatat tctgcttgcc
481 gttggtgagg tcacgaagtc cattgcgggc ggcaagataa ctgcgagtat caaatggggg
541 agccccgtgt cactgaagaa gttttttccg gcagaaatgg aaggcatgca cacaaatcaa
601 tttcttggcg gcgcgggtgt tgccattgtg gcgtccaacg ggaatcttgt gtaccctgtg
661 caggttacga acaaaaggaa gcaagttttc tccaagatct tctactcgga agatgatggg
721 aagacgtgga agtttgggaa gggtaggagc gattttggct gctctgaacc tgtggccctt
781 gagtgggagg ggaagctcat cataaacacc cgagttgact ggaaacgccg tctggtgtac
841 gagtccagtg acatggggaa tacgtgggtg gaggctgtcg gcacgctctc gcgtgtgtgg
901 ggcccctcac caaaatcgga ccatcccggc agtcagagca gcttcactgc cgtgaccatc
961 gaaggaatgc gtgtgatgct cttcacacac ccgctgaatt ttaagggaag gtggctgcgc
1021 gaccgactga acctctggct gacggataac cagcgcattt ataacgttgg gcaagtatcc
1081 attggtgatg aaaattccgc ctacagctcc gtcctgtaca aggatgataa gctgtactgt
1141 ttgcatgaga tcaacacgga cgaggtgtac agccttgttt ttgcacgcct ggttggcgag
1201 ctacggatca ttaaatcagt gctgcgatcc tggaagaatt gggacagcca cctgtccagc
1261 atttgcaccc ctgctgatcc agccgcttcg tcgtcagaga gtggttgtgg tcccgctgtc
1321 accacggttg gtcttgttgg ctttttgtcc ggcaatgcct cccaaaacgt atgggaggat
1381 gcgtaccgct gcgtcaacgc aagcacggca aatgcggaga gggttcggaa cggtttgaag
1441 tttgcggggg ttggcggagg ggcgctttgg ccggtgagcc agcaggggca gaatcagcgg
1501 tatcgttttg caaaccacgc gttcacgctg gtggcgtcgg tgacgattca cgaggctccg
1561 agggccgcga gtcctttgct gggtgcgagc ctggactctt ctggtggcaa aaaactcctg
1621 gggctctcgt acgacgagaa gcaccagtgg cagccaatat acggatcaac gccggtgacg
1681 ccgacgggat cgtgggagac gggtaagagg taccacgtgg ttcttacggt ggcgaataaa
1741 attggctccg tgtacattga tggagaactt ctggagggtt caggacagac cgttgtgcca
1801 gacgggagga cgcctgacat ctcccacttc tacgttggcg ggtatggaag gagtgatatg
1861 ccaaccataa gccacgtgac ggtgaataat gttcttcttt acaaccgaca gctgaatacc
1921 gaggagatca ggaccttgtt cttgagccag gacctgattg gcacggaagc acacatggac
1981 agcagcagcg acacgagtgc ctgatcaggc ctaggcccca atgccctggc tcacaaatac
2041 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
2101 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
2161 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
2221 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
2281 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
2341 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
2401 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
2461 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc
2521 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
2581 catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca
2641 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
2701 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
2761 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
2821 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
2881 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
2941 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
3001 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
3061 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
3181 ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
3241 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
3301 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
3361 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
3421 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
3481 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
3541 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
3601 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
3661 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
3721 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
3781 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
3841 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
3901 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
3961 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
4021 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
4081 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
4141 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
4201 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
4261 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
4321 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
4381 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
4441 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
4501 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
4561 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
4621 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
4681 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
4741 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
4801 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
4861 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
4921 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
4981 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta
5041 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
5101 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
5161 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
5221 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
5281 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
5341 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
5401 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
5461 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
5521 gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
5581 cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
5641 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg
5701 cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
5761 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
5821 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
5881 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
5941 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
6001 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
6061 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
6121 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
6181 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
6241 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
6301 gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
6361 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
6421 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
6481 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
6541 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
6601 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
6661 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
6721 gctggttatt gtgctgtctc atcattttgg caaagaa
//