LOCUS LT727315 5332 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAGmA20f8, complete sequence.
ACCESSION LT727315
VERSION LT727315.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAGmA20f8
ORGANISM Mammalian expression vector pCAGGS-FLAGmA20f8
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5332)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5332
/organism="Mammalian expression vector pCAGGS-FLAGmA20f8"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4127."
/db_xref="taxon:1944860"
misc_feature 13..36
/note="FLAG epitope"
CDS <49..606
/codon_start=1
/transl_table=11
/note="mA20 (591-775)"
/protein_id="SJL88084.1"
/translation="KCRKAGCMYFGTPENKGFCTLCFIEYRENKQSVTASAKAGSPAP
RFQNNVPCLGRECGTLGSTMFEGYCQKCFIEAQNQRFHEARRTEEQLRSSQHRDMPRT
TQVASRPKCARASCKNILACRSEELCMECQHLSQRVGSVAHRGEPTPEEPPKQRCRAP
ACDHFGNAKCNGYCNECYQFKQMYG"
regulatory 618..709
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 710..1066
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1144..1226)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1368..1443
/note="SV40 ori"
regulatory 1474..1548
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1474..1548)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(1663..2064)
/note="pMB1 ori"
CDS complement(2605..3465)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88085.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3605..3982
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3988..5329
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4357..5281
/note="intron AG promoter"
BASE COUNT 1151 a 1405 c 1483 g 1293 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatgaa gtgcaggaaa
61 gctggctgca tgtattttgg gactccagaa aacaagggct tttgcactct atgtttcatc
121 gaatacagag aaaataagca gtctgttact gcctctgcga aagctggttc cccggccccc
181 aggttccaga acaatgtccc gtgcctgggc agggagtgcg gcacactcgg aagcaccatg
241 tttgaagggt actgtcagaa gtgtttcatc gaagctcaga accagagatt ccatgaagca
301 agaagaacgg aagaacagct gagatcaagc cagcatagag acatgcctcg aactacacag
361 gtagcctcaa ggccgaaatg tgcccgggcc tcctgcaaga acattctggc ctgtcgcagt
421 gaggaactct gtatggagtg ccagcaccta agccaacgag taggttctgt ggcccaccgg
481 ggtgagccca cgcctgaaga gccccctaaa cagcgctgcc gggcccctgc ttgtgatcac
541 tttggcaatg ccaagtgtaa tggttactgc aatgagtgct accagttcaa gcagatgtat
601 ggctaagtgt cgacgaagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
661 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
721 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
781 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
841 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
901 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
961 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
1021 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc
1081 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
1141 attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc taatgagtga
1201 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
1261 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
1321 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
1381 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
1441 cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt
1501 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
1561 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat
1621 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
1681 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
1741 cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt gagcaaaagg
1801 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
1861 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
1921 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
1981 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
2041 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
2101 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
2161 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
2221 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
2281 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
2341 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
2401 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
2461 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
2521 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
2581 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
2641 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
2701 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
2761 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
2821 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
2881 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
2941 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
3001 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
3061 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
3121 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
3181 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
3241 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
3301 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
3361 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
3421 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
3481 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
3541 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc
3601 gatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
3661 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
3721 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
3781 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
3841 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
3901 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
3961 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
4021 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
4081 gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg
4141 cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
4201 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
4261 gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc
4321 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
4381 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
4441 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
4501 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
4561 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
4621 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
4681 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
4741 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
4801 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
4861 gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc
4921 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
4981 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
5041 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
5101 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
5161 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
5221 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
5281 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aa
//