LOCUS LT727314 5464 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAGmA20f7, complete sequence.
ACCESSION LT727314
VERSION LT727314.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAGmA20f7
ORGANISM Mammalian expression vector pCAGGS-FLAGmA20f7
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5464)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5464
/organism="Mammalian expression vector pCAGGS-FLAGmA20f7"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4126."
/db_xref="taxon:1944859"
misc_feature 13..36
/note="FLAG epitope"
CDS <49..738
/codon_start=1
/transl_table=11
/note="mA20 (547-775)"
/protein_id="SJL88082.1"
/translation="RSKSDPSQLIQSLTPHSCHRTGNVSPSGCLSQAARTPGDRAGTS
KCRKAGCMYFGTPENKGFCTLCFIEYRENKQSVTASAKAGSPAPRFQNNVPCLGRECG
TLGSTMFEGYCQKCFIEAQNQRFHEARRTEEQLRSSQHRDMPRTTQVASRPKCARASC
KNILACRSEELCMECQHLSQRVGSVAHRGEPTPEEPPKQRCRAPACDHFGNAKCNGYC
NECYQFKQMYG"
regulatory 750..841
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 842..1198
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1276..1358)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1500..1575
/note="SV40 ori"
regulatory 1606..1680
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1606..1680)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(1795..2196)
/note="pMB1 ori"
CDS complement(2737..3597)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88083.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3737..4114
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4120..5461
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4489..5413
/note="intron AG promoter"
BASE COUNT 1181 a 1455 c 1510 g 1318 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatgcg ctccaagtct
61 gacccctcac aactcatcca aagtctcact ccacactctt gccaccggac tggaaatgtc
121 tctccttctg gctgcctctc ccaggctgca cggactccag gagacagagc agggacaagc
181 aagtgcagga aagctggctg catgtatttt gggactccag aaaacaaggg cttttgcact
241 ctatgtttca tcgaatacag agaaaataag cagtctgtta ctgcctctgc gaaagctggt
301 tccccggccc ccaggttcca gaacaatgtc ccgtgcctgg gcagggagtg cggcacactc
361 ggaagcacca tgtttgaagg gtactgtcag aagtgtttca tcgaagctca gaaccagaga
421 ttccatgaag caagaagaac ggaagaacag ctgagatcaa gccagcatag agacatgcct
481 cgaactacac aggtagcctc aaggccgaaa tgtgcccggg cctcctgcaa gaacattctg
541 gcctgtcgca gtgaggaact ctgtatggag tgccagcacc taagccaacg agtaggttct
601 gtggcccacc ggggtgagcc cacgcctgaa gagcccccta aacagcgctg ccgggcccct
661 gcttgtgatc actttggcaa tgccaagtgt aatggttact gcaatgagtg ctaccagttc
721 aagcagatgt atggctaagt gtcgacgaag atctttttcc ctctgccaaa aattatgggg
781 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
841 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
901 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
961 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
1021 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
1081 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
1141 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
1201 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
1261 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg
1321 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
1381 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
1441 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
1501 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
1561 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
1621 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
1681 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
1741 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
1801 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
1861 ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat
1921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
1981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
2041 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
2101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
2161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
2221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
2281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
2341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
2401 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
2461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
2521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
2581 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
2641 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
2701 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
2761 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
2821 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
2881 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
2941 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
3001 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
3061 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
3121 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
3181 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
3241 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
3301 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
3361 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
3421 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
3481 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
3541 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
3601 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
3661 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
3721 gccacctggg tcgatcgaca ttgattattg actagttatt aatagtaatc aattacgggg
3781 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
3841 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
3901 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
3961 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
4021 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
4081 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
4141 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
4201 ttattttgtg cagcgatggg ggcggggggg gggggggggc cccccccagg cggggcgggg
4261 cggggcgagg ggcggggcgg ggcgaggcgg aaaggtgcgg cggcagccaa tcagagcggc
4321 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
4381 agcgcgcggc gggcgggagt cgttgcgcgc tgccttcccc ccgtgccccg ctccgccgcc
4441 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
4501 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
4561 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
4621 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
4681 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
4741 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
4801 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
4861 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
4921 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
4981 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaagg ggcgcggcgg
5041 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
5101 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
5161 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
5221 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
5281 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
5341 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
5401 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
5461 agaa
//