LOCUS LT727313 6694 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-FLAGmA20f2, complete sequence. ACCESSION LT727313 VERSION LT727313.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-FLAGmA20f2 ORGANISM Mammalian expression vector pCAGGS-FLAGmA20f2 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6694) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6694 /organism="Mammalian expression vector pCAGGS-FLAGmA20f2" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4122." /db_xref="taxon:1944855" misc_feature 13..36 /note="FLAG epitope" CDS 46..>1965 /transl_table=11 /note="mA20 (1-640)" /protein_id="SJL88080.1" /translation="MAEQLLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIYHFKTMH RYTLEMFRTCQFCPQFREIIHKALIDRSVQASLESQKKLNWCREVRKLVALKTNGDGN CLMHAACQYMWGVQDTDLVLRKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYD TRNWNDEWDNLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDKMLRSL ESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPELRAVPLV NRDRGRFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDHGTTHLINAAKLDEAN LPKEINLVDDYFELVQHEYKKWQENSDQARRAAHAQNPLEPSTPQLSLMDIKCETPNC PFFMSVNTQPLCHECSERRQKNQSKLPKLNSKLGPEGLPGVGLGSSNWSPEETAGGPH SAPPTAPSLFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHARQINASHTADPGKCQA CLQDVTRTFNGICSTCFKRTTAEPSSSLTSSIPASCHQRSKSDPSQLIQSLTPHSCHR TGNVSPSGCLSQAARTPGDRAGTSKCRKAGCMYFGTPENKGFCTLCFIEYRENKQSVT ASAKAGSPAPRFQNNV" regulatory 1980..2071 /note="3' UTR of beta-globin, incl. polyA" /regulatory_class="terminator" misc_feature 2072..2428 /note="3' flanking region of rabbit beta-globin" regulatory complement(2506..2588) /note="lac promoter" /regulatory_class="promoter" rep_origin 2730..2805 /note="SV40 ori" regulatory 2836..2910 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(2836..2910) /note="SV40 polyA late" /regulatory_class="terminator" rep_origin complement(3025..3426) /note="pMB1 ori" CDS complement(3967..4827) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL88081.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 4967..5344 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 5350..6691 /note="AG promoter" /regulatory_class="promoter" misc_feature 5719..6643 /note="intron AG promoter" BASE COUNT 1531 a 1774 c 1778 g 1611 t ORIGIN 1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt 61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc 121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga 181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac 241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg 301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg 361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc 421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa 481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat 541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga 601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga 661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct 721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga 781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac 841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac 901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag 961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc 1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac 1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga 1141 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata 1201 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac 1261 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta 1321 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc 1381 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc 1441 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga 1501 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag 1561 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc 1621 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc ctcctgtcac 1681 caacgctcca agtctgaccc ctcacaactc atccaaagtc tcactccaca ctcttgccac 1741 cggactggaa atgtctctcc ttctggctgc ctctcccagg ctgcacggac tccaggagac 1801 agagcaggga caagcaagtg caggaaagct ggctgcatgt attttgggac tccagaaaac 1861 aagggctttt gcactctatg tttcatcgaa tacagagaaa ataagcagtc tgttactgcc 1921 tctgcgaaag ctggttcccc ggcccccagg ttccagaaca atgtctaagt gtcgacgaag 1981 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt 2041 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct 2101 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt 2161 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag 2221 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga 2281 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa 2341 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca 2401 tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca 2461 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 2521 gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc acattaattg 2581 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct 2641 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 2701 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 2761 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 2821 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc 2881 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 2941 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg 3001 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 3061 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 3121 cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 3181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 3241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 3301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 3361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 3421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 3481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 3541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 3601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 3661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 3721 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 3781 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 3841 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 3901 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 3961 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 4021 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 4081 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 4141 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 4201 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 4261 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 4321 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 4381 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 4441 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 4501 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 4561 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 4621 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 4681 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 4741 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 4801 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 4861 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 4921 aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgatcgaca ttgattattg 4981 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc 5041 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 5101 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 5161 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 5221 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 5281 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 5341 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca 5401 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg 5461 gggggggggc cccccccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg 5521 aaaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg 5581 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgttgcgcgc 5641 tgccttcccc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg 5701 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag 5761 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc 5821 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg 5881 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg 5941 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg 6001 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga 6061 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt 6121 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc 6181 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc 6241 ggggagggct cgggggaagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc 6301 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt 6361 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg 6421 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc 6481 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg 6541 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag 6601 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct 6661 ggttattgtg ctgtctcatc attttggcaa agaa //