LOCUS LT727311 5344 bp DNA circular SYN 06-FEB-2017
DEFINITION Suicide vector pVIK165, complete sequence.
ACCESSION LT727311
VERSION LT727311.1
KEYWORDS .
SOURCE Suicide vector pVIK165
ORGANISM Suicide vector pVIK165
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5344)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5344
/organism="Suicide vector pVIK165"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4119."
/db_xref="taxon:1945234"
misc_feature 73..78
/note="synthetic RBS"
CDS 86..802
/transl_table=11
/note="GFP mutant"
/protein_id="SJL88076.1"
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD
DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
VLLEFVTAAGITHGMDELYK"
misc_feature 278..280
/note="S65T"
CDS 1424..2218
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL88077.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
rep_origin 4179..4289
/note="RK2/RP4 oriT"
rep_origin 4901..5288
/note="R6K ori"
BASE COUNT 1254 a 1431 c 1480 g 1179 t
ORIGIN
1 gaattcccgg gagagctcga tatcgcatgc ggtacctcta gaagaagctt gggatccgtc
61 gacctcgaat tcggaggaaa caaagatgag taaaggagaa gaacttttca ctggagttgt
121 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
181 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
241 actacctgtt ccatggccaa cacttgtcac tactttcact tatggtgttc aatgcttttc
301 aagataccca gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
361 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
421 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
481 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
541 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga
601 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
661 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
721 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
781 catggatgaa ctatacaaat aaatgtccag acttccaatt cccgggccta tcaagcttat
841 cgataccgtc gacgttactc tttccgttac gggacaccct gtacaccatg aattgagaaa
901 aaacggcgag atgtactctt ttccgataac gattggcaat aacgtctgga tcggaagtca
961 tgtggttatt aatccaggcg tcaccatcgg ggataattct gttattggcg cgggtagtat
1021 cgtcacaaaa gacattccac caaacgtcgt ggcggctggc gttccttgtc gggttattcg
1081 ccgcaagcac tcagggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc
1141 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa
1201 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg
1261 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt
1321 tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag
1381 gggatcaaga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
1441 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
1501 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
1561 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
1621 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
1681 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
1741 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
1801 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
1861 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
1921 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
1981 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
2041 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
2101 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
2161 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
2221 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
2281 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
2341 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc cgcaatggca
2401 acaacgcgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
2461 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
2521 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
2581 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
2641 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
2701 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
2761 tttaattttg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
2821 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
2881 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
2941 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagca
3001 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
3061 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
3121 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
3181 ggatccagcc gaccaggctt tccacgcccg cgtgccgctc catgtcgttc gcgcggttct
3241 cggaaacgcg ctgccgcgtt tcgtgattgt cacgctcaag cccgtagtcc cgttcgagcg
3301 tcgcgcagag gtcagcgagg gcgcggtagg cccgatacgg ctcatggatg gtgtttcggg
3361 tcgggtgaat cttgttgatg gcgatatgga tgtgcaggtt gtcggtgtcg tgatgcacgg
3421 cactgacgcg ctgatgctcg gcgaagccaa gcccagcgca gatgcggtcc tcaatcgcgc
3481 gcaacgtctc cgcgtcgggc ttctctcccg cgcggaagct aaccagcagg tgataggtct
3541 tgtcggcctc ggaacgggtg ttgccgtgct gggtcgccat cacctcggcc atgacagcgg
3601 gcagggtgtt tgcctcgcag ttcgtgacgc gcacgtgacc caggcgctcg gtcttgcctt
3661 gctcgtcggt gatgtacttc accagctccg cgaagtcgct cttcttgatg gagcgcatgg
3721 ggacgtgctt ggcaatcacg cgcacccccc ggccgtttta gcggctaaaa aagtcatggc
3781 tctgccctcg ggcggaccac gcccatcatg accttgccaa gctcgtcctg cttctcttcg
3841 atcttcgcca gcagggcgag gatcgtggca tcaccgaacc gcgccgtgcg cgggtcgtcg
3901 gtgagccaga gtttcagcag gccgcccagg cggcccaggt cgccattgat gcgggccagc
3961 tcgcggacgt gctcatagtc cacgacgccc gtgattttgt agccctggcc gacggccagc
4021 aggtaggccg acaggctcat gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg
4081 tctggaaggc agtacacctt gataggtggg ctgcccttcc tggttggctt ggtttcatca
4141 gccatccgct tgccctcatc tgttacgccg gcggtagccg gccagcctcg cagagcagga
4201 ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg
4261 ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct
4321 acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa
4381 atcgctataa tgaccccgaa gcagggttat gcagcggaaa agcgctgctt ccctgctgtt
4441 ttgtggaata tctaccgact ggaaacaggc aaatgcagga aattactgaa ctgaggggac
4501 aggcgagaga cgatgccaaa gagctacacc gacgagctgg ccgagtgggt tgaatcccgc
4561 gcggccaaga agcgccggcg tgatgaggct gcggttgcgt tcctggcggt gagggcggat
4621 gtcgaggcgg cgttagcgtc cggctatgcg ctcgtcacca tttgggagca catgcgggaa
4681 acggggaagg tcaagttctc ctacgagacg ttccgctcgc acgccaggcg gcacatcaag
4741 gccaagcccg ccgatgtgcc cgcaccgcag gccaaggctg cggaacccgc gccggcaccc
4801 aagacgccgg agccacggcg gccgaagcag gggggcaagg ctgaaaagcc ggcccccgct
4861 gcggccccga ccggcttcac cttcaaccca acaccggaca aaaaggatcc agcagttcaa
4921 cctgttgata gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt
4981 actaagctct catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc
5041 taacgtacta agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa
5101 tataaatcag caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata
5161 tataaggctt ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac
5221 gcactgagaa gcccttagag cctctcaaag caattttcag tgacacagga acacttaacg
5281 gctgacatgg gaattccaca tgtggaattc cacatgtgga attgtgagcg gataacaatt
5341 tgtg
//