LOCUS LT727305 5667 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pBW4, complete sequence.
ACCESSION LT727305
VERSION LT727305.1
KEYWORDS .
SOURCE Vector pBW4
ORGANISM Vector pBW4
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5667)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5667
/organism="Vector pBW4"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4112."
/db_xref="taxon:1945266"
rep_origin 388..761
/note="pPIGDM1 ori"
CDS 1751..2410
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL88048.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS <4791..>4976
/codon_start=1
/transl_table=11
/note="rom"
/protein_id="SJL88049.1"
/translation="SRIFLAYLCVQHGVKRFGLIVQVLTAAGSLIQIHLLEPLKEEKC
LCADKFGHIEGGGFLSGH"
BASE COUNT 1289 a 1430 c 1489 g 1459 t
ORIGIN
1 gttaggcgcg aggtgctatg gtcaaagtgt ggtgtcaggc ggggaggagg cttatatgag
61 atgtaatccg gccgatacat ataccaagat gatttaagta ttcctctttg tgaggttgga
121 tgtctaattt ataaaggatc ttcttgagat cctttttttc cgcgcgtaat ctcttgccct
181 gtaaacgaaa aaaccaccct ggcaggtggt ttttcgaagg ttaggtaatc ctggcagatc
241 ccctaaccgt ggtaacagtc ttgtgcgaga catgtcacca aatttgtcct ttcagtgtag
301 cctcactaag gccgccactt caagaactct tgagacatct ctcgcacatc ctgtttgcca
361 atggccgttg ccaatggcga ttagtcgtgt ctttccgggt tggactcaag ttgatagtta
421 ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc catcctggag
481 cgaactgcct tcccggaact gagtgtcagg cgtggaatga gaaaccgcgg ccataacagc
541 ggagtgacac cggtaaaccg aaaggcagga atgcggggga gcacgaggga gccaccaggg
601 ggaaacgcct ggtatcttta agccgcatcg ggtttcgcca ccactgattt gagcgtcaga
661 ttctgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttggc gcggccttat
721 gctttcttcg ttaagtatct tcctggcatc ccccaggaaa tttctgatcc atccgtaagc
781 ccgtcccgct cgccgcagcc gaacgaccga gcggagcgag tcagtgagcg aggaagcgga
841 atatattctg tatcacattt tctcctgacg cgttttcttt cactttctgc gcctgtctta
901 tgtggcatta atgctatgtg ttactgccat gctacatctt aagccagtat acactccgct
961 agtgctccgt gactggtccg gcgctgcgcc cggaacccgc ctgtaccggt tcagcagccg
1021 ttccggcctg actgcaattt tttttttttc atccctgccc gctaccctgt aaacctttct
1081 tctgcgttgc cgttaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
1141 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
1201 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
1261 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
1321 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
1381 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
1441 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
1501 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcgaataaa tacctgtgac
1561 ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg
1621 ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca
1681 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
1741 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
1801 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
1861 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
1921 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
1981 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
2041 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
2101 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
2161 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
2221 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
2281 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
2341 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
2401 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga
2461 ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg
2521 gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga
2581 ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc
2641 tccccgtgga ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa
2701 aaacggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct
2761 ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca
2821 tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc
2881 agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc
2941 gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc
3001 gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta
3061 gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt
3121 ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga
3181 cttctcatgt ttgacagctt atcatcgata agctttaatg cggtagttta tcacagttaa
3241 attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg
3301 gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct
3361 tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat
3421 atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc
3481 gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga
3541 ccacacccgt cctgtggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
3601 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
3661 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
3721 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
3781 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcaacggc
3841 cagggcagcg atgcgaacgt ttacctgtct ccggtaaccc tgactgaagt taaagcggca
3901 gcagcggata cttacctgaa accgaaatct ttcaccattg acgtctctaa ctgccaggct
3961 gctgatggca ctaaacagga tgacgtaagc aaactgggcg tgaactggac cggcggtaac
4021 ctgctggctg gcgcaaccag caaacaacag ggctacctgg cgaacaccga agcttccggt
4081 gctcagaaca tccagctggt gctgtctact gacaacgcta ccgcgctgac caacaaaatc
4141 atcccgggtg acagcactca gccgaaagcg aaaggcgacg cttccgctgt agctgatggc
4201 gcgcgtttca cctactacgt aggctacgcc accagcgcgc cgaccacggt aaccaccggt
4261 gtggttaaca gtaggtaagc catgtttggt ttttcttccg gagccctgat gactcttagt
4321 gatgatgagc gacacctgct tgtgtccgtt gtttcggtct ggctacggcg tgccggaggg
4381 gatgccggag ccatgatgct ggatgcatac cggcagattt tgtctgaaac cgaaccggcc
4441 gtaaggactg tcatgctcga atttcttgaa tccgttcgca ttcattacat ttcgtcctga
4501 gcattttctg tatttctgtc catacttaaa cgaggcggcc gtcacggacg gtggtcgtcg
4561 ttccatgatg ctgttatgga cgagtgagag taatctaatc taacttgatt gagttccggg
4621 cgtgccgccc ggtttttttt gctgtttccc ggtgtccgtt ccgggacagg atgtgcattt
4681 gtaataccgc gcacgcgccg gcatcacaaa taccctggtc agggctgcgc cccgacaccc
4741 cgccatcgcc tcacggccgc gcactgaatt gcgcacccgt tcaccgattt tcacgcattt
4801 tcctcgccta tctgtgcgtt cagcatggcg taaagcgctt cggcctgatc gtgcaggtgc
4861 tcacagcagc cggcagcctc atccagatcc atctgctcga gccgctcaag gaggagaagt
4921 gtctgtgcgc ggataaattt ggccatattg agggcggcgg attcctgtct ggtcactggt
4981 gattttcccc gtctggtgat gatttttgcg tgaaaagagc atggcgtcat tctggtgcat
5041 agtcatgcat gccgttaaaa tttaacaggc gcgtttctga cgggttccgg ggtggtttgt
5101 tgtggttttt gtcatggttt cgtcaggaac ccgctgaacg cgtctcaggc ggtgcgggca
5161 acggatgtta tggtaaattc ttgggggctt gattataaaa taccgcactt cctcttatgt
5221 cttgtgcttt ttagacaacc ctgaaagctt ttagctttgg tgatgcaatt tgctgggatt
5281 ttggcggaga accacaggta aagaaaaagg ccacattagc ggcctttttc ggagaagatg
5341 ctcagtgcgg aatttcggag acttctttaa cgtcggtttt attgatctgc tgtttgacac
5401 cattcgcgtc tgtatagcca agcaggcctg agtctgattc tttaggtttg ccgtcgctga
5461 caatagtgcg accgtcattg gtgtgtatgg catagcttgt gcgcgtgcag ccggtgaggg
5521 ctgaaagcgc aactgccgct gcgaacactg aaacaaaaat ctttttcaaa gccagctcct
5581 tttatccata tcagttttgg tcactaacca tctaactata ggtcattttt tatgtgaaaa
5641 gaggcttgat ggtgggctgt atatcga
//