LOCUS LT727304 7176 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pGAL1PNiST-1, complete sequence.
ACCESSION LT727304
VERSION LT727304.1
KEYWORDS .
SOURCE Yeast expression vector pGAL1PNiST-1
ORGANISM Yeast expression vector pGAL1PNiST-1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7176)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7176
/organism="Yeast expression vector pGAL1PNiST-1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4099."
/db_xref="taxon:1945482"
regulatory complement(12..28)
/note="SP6 promoter"
/regulatory_class="promoter"
regulatory complement(89..172)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(264..666)
/note="pMB1 ori"
CDS complement(1207..2067)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88045.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin complement(2506..2961)
/note="f1 ori"
regulatory 3125..3141
/note="T7 promoter"
/regulatory_class="promoter"
CDS 3692..4504
/transl_table=11
/note="Candida URA3"
/protein_id="SJL88046.1"
/translation="MTVNTKTYSERAETHASPVAQRLFRLMELKKTNLCASIDVDTTK
EFLELIDKLGPYVCLIKTHIDIINDFSYESTIEPLLELSRKHQFMIFEDRKFADIGNT
VKKQYIGGVYKISSWADITNAHGVTGNGVVEGLKQGAKETTTNQEPRGLLMLAELSSV
GSLAYGEYSQKTVEIAKSDKEFVIGFIAQRDMGGQEEGFDWLIMTPGVGLDDKGDGLG
QQYRTVDEVVSTGTDIIIVGRGLFGKGRDPDIEGKRYRDAGWNAYLKKTGQL"
regulatory 4799..6011
/note="CaGAL1 promoter"
/regulatory_class="promoter"
misc_feature complement(6069..6268)
/note="3' UTR of hIFNbeta"
CDS complement(6269..6832)
/transl_table=11
/note="hIFNbeta"
/protein_id="SJL88047.1"
/translation="MTNKCLLQIALLLCFSTTALSMSYNLLGFLQRSSNFQCQKLLWQ
LNGRLEYCLKDRMNFDIPEEIKQLQQFQKEDAALTIYEMLQNIFAIFRQDSSSTGWNE
TIVENLLANVYHQINHLKTVLEEKLEKEDFTRGKLMSSLHLKRYYGRILHYLKAKEYS
HCAWTIVRVEILRNFYFINRLTGYLRN"
regulatory 6906..7176
/note="CYC1 terminator"
/regulatory_class="terminator"
BASE COUNT 2084 a 1436 c 1600 g 2056 t
ORIGIN
1 cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc
61 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
121 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
181 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
241 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
301 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
361 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
421 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
481 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
541 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
601 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
661 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
721 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
781 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
841 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
901 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
961 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
1021 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
1081 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
1141 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
1201 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
1261 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
1321 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
1381 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
1441 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
1501 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
1561 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
1621 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
1681 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
1741 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
1801 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
1861 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
1921 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
1981 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
2041 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
2101 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
2161 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
2221 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
2281 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
2341 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
2401 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
2461 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgaaatt gtaaacgtta
2521 atattttgtt aaaattcgcg ttaaatattt gttaaatcag ctcatttttt aaccaatagg
2581 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
2641 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
2701 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgc
2761 ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga tttagagctt
2821 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
2881 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
2941 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
3001 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
3061 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
3121 attgtaatac gactcactat agggcgaatt ggttttccaa tgatgagcac ttttaaagtt
3181 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
3241 atacactatt ctcagaatga cttggttgag tactaatagg aattgatttg gatggtataa
3301 acggaaacaa aaaaaagagc tggtactact ttctttaaaa ttattttatt atttgatttt
3361 atttaatagt atatattata ttttgaacgt agattatttt gttgaaagtt gctgtagtgc
3421 cattgattcg taacactaat tctgtattag tcattcctct tgtttgatag tatccaaaaa
3481 aacggctatt tttttgcaat cttatttcct gcatattata cagataacat aatgaaagaa
3541 aaaatctttt tttttgttct tcaatgatga tttcaaccat tcttttaaac attgatcaat
3601 tcctgagcaa caaccccata cacactggtt tatatatcgc cccttttaca gttgaagaaa
3661 gaaatagaaa tagaaatagc aaacaaaaga tatgacagtc aacactaaga cctatagtga
3721 gagagcagaa actcatgcct caccagtagc acaacgatta tttcgattaa tggaactgaa
3781 gaaaaccaat ttatgtgcat caattgatgt tgataccact aaggaattcc ttgaattaat
3841 tgataaattg ggtccttatg tatgcttaat caagactcat attgatataa tcaatgattt
3901 ttcctatgaa tccactattg aaccattatt agaactttca cgtaaacatc aatttatgat
3961 ttttgaagat agaaaatttg ctgatattgg taataccgtg aagaaacaat atattggtgg
4021 agtttataaa attagtagtt gggcagatat tactaatgct catggtgtca ctgggaatgg
4081 agtagttgaa ggattaaaac agggagctaa agaaaccacc accaaccaag agccaagagg
4141 gttattgatg ttagctgaat tatcatcagt gggatcatta gcatatggag aatattctca
4201 aaaaactgtt gaaattgcta aatccgataa ggaatttgtt attggattta ttgcccaacg
4261 tgatatgggt ggacaagaag aaggatttga ttggcttatt atgacacctg gagttggatt
4321 agatgataaa ggtgatggat taggacaaca atatagaact gttgatgaag ttgttagcac
4381 tggaactgat attatcattg ttggtagagg attgtttggt aaaggaagag atccagatat
4441 tgaaggtaaa aggtatagag atgctggttg gaatgcttat ttgaaaaaaa ctggccaatt
4501 ataaatgtga agggggagat tttcacttta ttagatttgt atatatgtat aataaataaa
4561 taaataagtt aaataaataa ttaaataagg gtggtaatta ttactattta caatcaaagg
4621 tggtccttct agctgtaatc cgggcagcgc aacggaacat tcatcagtgt aaaaatggaa
4681 tcaataaagc cctgcgcagc gcgcagggtc agcctgaata cgcgtttaat gaccagcaca
4741 gtcgtgatgg caaggtcaga atagcccaag tcggccgagg ggcctgtaca gtgagggaag
4801 atctgatatt gacgaagagg aaccaatgta acgttacact gaagaaaaca cacaataaac
4861 gggaagaaac ggtgtaaaag tgtgaaaata atttttgaat atcatttccc ttggtttaat
4921 tccaaacgaa acgtgttttt tttagagaat gggaattctt attggatgtc tagattgttt
4981 gtttactcca gactgtgcac aaaaacgttt ggatggatga tcagaagata tttttaggct
5041 tagctctaaa tataagaaat gatgcttgaa aaaccagaca gaaattgagt ttcaaaaatt
5101 ggtaatgtga ggtattagtc aactaaccaa ataacaatgc aaaccggttg atacatttca
5161 ttttgaaaat aatgaaactg gaattggatg accagcacac aaacacataa agtaattatg
5221 ggaattagaa gcgaacatag aggagtactt ggccacgaac agaatacaag tgggaacact
5281 attttctcca ttgttttagt tctgtttttt tgtcagccta gttttgtgct atgtgtaaaa
5341 aatattgcca agaaaaaaag cttgttttgt ggccagtgtc cgaaaaaaat tttggggaat
5401 cttcggatta atttatgttt tcattccatc ggggaaagtg ggggggaaaa aattttaagc
5461 agttcacaaa accttccaaa aaatatatgg acaaagatga ttgtattttc ccgacaccaa
5521 aatcataatt aattatgaga aagttaaatg taacgttaca atttatgttt atttgaaggt
5581 gaaaagcgat ttatgatttt tccgaaatga aaattttttt taggtttatt ttttttgtcg
5641 ggcaaagaaa aactgaacaa ggattattaa aatttttggt gtttgtttgt gtctggagaa
5701 ttcattcctc tctcatcttc acacaatgtt tagacatctg acacgattca tgatagttcg
5761 gtttccgggg ttggtgttta gttttcgttt ttcttttttt ttggaaagaa tgttttagct
5821 cattggtttt ctttcttcat tcaatagttt tgaaagaatt tgcccacttg ttattacaat
5881 catataaaat taaactttga tataaaatag agtttgaaag tttcccagat cctttttgat
5941 ttctttgtaa attttttttt ctcccacata tacacacata caaaccgatt tttataagaa
6001 agagttatac cctgcagctc gacctcgagg gatccgggcc ctctagatgc ggccgctagg
6061 cctcgaggga cttttgcacc aaaaataatt tattttccaa aataaaattt aaataaataa
6121 aaataactca taatttaata aaaatttcaa aatcttctag tgtcctttca tatgcagtac
6181 attagccatc agtcacttaa acagcatctg ctggttgaag aatgcttgaa gcaattgtcc
6241 agtcccagag gcacaggcta ggagatcttc agtttcggag gtaacctgta agtctgttaa
6301 tgaagtaaaa gttccttagg atttccactc tgactatggt ccaggcacag tgactgtact
6361 ccttggcctt caggtaatgc agaatcctcc cataatatct tttcaggtgc agactgctca
6421 tgagttttcc cctggtgaaa tcttctttct ccagtttttc ttccaggact gtcttcagat
6481 ggtttatctg atgatagaca ttagccagga ggttctcaac aatagtctca ttccagccag
6541 tgctagatga atcttgtctg aaaatagcaa agatgttctg gagcatctca tagatggtca
6601 atgcggcgtc ctccttctgg aactgctgca gctgcttaat ctcctcaggg atgtcaaagt
6661 tcatcctgtc cttgaggcag tattcaagcc tcccattcaa ttgccacagg agcttctgac
6721 actgaaaatt gctgcttctt tgtaggaatc caagcaagtt gtagctcatg gaaagagctg
6781 tagtggagaa gcacaacagg agagcaattt ggaggagaca cttgttggtc atgttcctcg
6841 aggccttttt ggccaagctg gcgcctgctg cgcgacggcg agctgctcac cacccaggat
6901 ccgtccccct tttcctttgt cgatatcatg taattagtta tgtcacgctt acattcacgc
6961 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
7021 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
7081 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
7141 aaggttttgg gacgctcgaa ggctttaatt tgcaag
//