LOCUS LT727301 10011 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV71BlahCH3, complete sequence.
ACCESSION LT727301
VERSION LT727301.1
KEYWORDS .
SOURCE Mammalian expression vector pSV71BlahCH3
ORGANISM Mammalian expression vector pSV71BlahCH3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 10011)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..10011
/organism="Mammalian expression vector pSV71BlahCH3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4082."
/db_xref="taxon:1945123"
regulatory 101..382
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 339..414
/note="SV40 ori"
misc_feature 453..698
/note="SV40 large T-antigen"
CDS 453..977
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL88033.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
misc_feature 699..1044
/note="intron"
misc_feature 1045..2925
/note="SV40 large T-antigen"
regulatory complement(2926..3022)
/note="polyA"
/regulatory_class="terminator"
regulatory 2926..3022
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(3096..3212)
/note="3UTR"
CDS complement(3213..>3533)
/codon_start=1
/transl_table=11
/note="CH3"
/protein_id="SJL88034.1"
/translation="QPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNG
QPENNYNTTPPMLDSDGSFFLYSKLTVDKSRWQQGNIFSCSVMHEALHNRFTQKSLSL
SPGK"
CDS complement(<3549..4406)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88035.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature complement(4520..5457)
/note="intron"
misc_feature complement(5458..5660)
/note="5UTR"
regulatory complement(5661..5956)
/note="SV40 late promoter"
/regulatory_class="promoter"
regulatory 5712..5993
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 5950..6025
/note="SV40 ori"
misc_feature 6064..6309
/note="SV40 large T-antigen"
CDS 6064..6588
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL88036.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
misc_feature 6310..6655
/note="intron"
misc_feature 6656..7718
/note="part of SV40 large t-antigen"
rep_origin complement(8005..8405)
/note="pMB1 ori"
CDS complement(9116..9775)
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL88037.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
BASE COUNT 2874 a 2096 c 2322 g 2719 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
3061 tgtatcttat catgtctgga tccggctcta gagtcgacct gggtgcttta tttccatgct
3121 gggtgcccgg gaagtatgta cacggggtac gtgccaagca tcctcgcgcg accccgagag
3181 cccggggagc gggggcttgc cggctgtcgc actcatttac ccggagacag ggagaggctc
3241 ttctgcgtga agcggttgtg cagagcctca tgcatcacgg agcatgagaa gatgttcccc
3301 tgctgccacc tgctcttgtc cacggtgagc ttgctgtaga ggaagaagga gccgtcggag
3361 tccagcatgg gaggcgtggt gttgtagttg ttctccggct gcccattgct ctcccactcc
3421 acggcgatgt cgctggggta gaagcctttg accaggcagg tcaggctgac ttggttcttg
3481 gtcatctcct cccgggatgg gggcagggtg tacacctgtg gttctcgggg ctggatcccc
3541 gggaattccc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
3601 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
3661 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
3721 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
3781 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
3841 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
3901 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
3961 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
4021 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
4081 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
4141 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
4201 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
4261 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
4321 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
4381 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcagacag
4441 ttttattgtt catgcctcta gaggctgcag ccgaccccgg atccggagct tttagagcag
4501 aagtaacact tccgtacagg cctagaagta aaggcaacat ccactgagga gcagttcttt
4561 gatttgcacc accaggagcc tcaaattttt caataaattc acctgactgc acattaggac
4621 tttggctttg agcttcccac ctctcagtta cttgctgaat actgtctgct tcatcaatat
4681 tatcataggt gtgcccaaat gatatttgca acccttccct gttggctact tgtctcacca
4741 ttgtaggcct aatgggagac aaagtagagt agtaatcttg taaagagtta taccaattaa
4801 caggagcatt aattactgtc caagtagttt cctctaaaaa ccttgccaaa ctgtccctta
4861 aatatctttg ggttcttctt tcaagctcct gtgaggtgag cctaggaatg tcattttgta
4921 ttacacgcca aaaagcttga gaaatggcat taaaaagtgt tggaccccaa tgtctggggt
4981 caagatactg aacactgtga acaaaggttt gtactccagg aaataaaata tcatagtaat
5041 catctggcct atacaaatct acagccattc ctggttgttg atataaacca acagtagaaa
5101 ctttgtgatc ccagtcacta aaaaatctat accccacttg agcaacagcg ctcacaccag
5161 tcacagtttg cagtaaagct gcaaatccag ctatagcagc aggagcccca gatatcacag
5221 cataggcctg tggagtgagg cctatagcag caattgcctc agaggttgtt aggccttcaa
5281 cagtagcaac agatgcaagt tgcacttcaa ttgcagcagc ggcctctcca gcagcaattt
5341 cagctactga aaatccagta gcagcagcag cttcagacac agtagcaatt aggtccccca
5401 acagtgttaa agcagcaccc atggacctga aataaaagac aaaaagacta aacttaccag
5461 ttaactttct ggtttttcag ttaacctttc tggttttttg cgtttcccgt caacagtatc
5521 ttccccttca caaaattgca gcaaaagctc taaaacaaac acaaaaaggc gttgagctgt
5581 ttttttactt tcagtccatg acctacgaac cttaacggag gcctggcgtg acagccggcg
5641 cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg
5701 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
5761 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
5821 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
5881 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
5941 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
6001 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctttgca
6061 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
6121 gaaaggagtg cctgggggaa tattcctctg atgagaaagg catatttaaa aaaatgcaag
6181 gagtttcatc ctgataaagg aggagatgaa gaaaaaatga agaaaatgaa tactctgtac
6241 aagaaaatgg aagatggagt aaaatatgct catcaacctg actttggagg cttctgggat
6301 gcaactgagg tatttgcttc ttccttaaat cctggtgttg atgcaatgta ctgcaaacaa
6361 tggcctgagt gtgcaaagaa aatgtctgct aactgcatat gcttgctgtg cttactgagg
6421 atgaagcatg aaaatagaaa attatacagg aaagatccac ttgtgtgggt tgattgctac
6481 tgcttcgatt gctttagaat gtggtttgga cttgatcttt gtgaaggaac cttacttctg
6541 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
6601 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
6661 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
6721 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
6781 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
6841 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
6901 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
6961 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
7021 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
7081 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatccat tttctgttat
7141 tgaggaaagt ttgccaggtg ggttaaagga gcatgatttt aatccagaag aagcagagga
7201 aactaaacaa gtgtcctgga agcttgtaac agagtatgca atggaaacaa aatgtgatga
7261 tgtgttgtta ttgcttggga tgtacttgga atttcagtac agttttgaaa tgtgtttaaa
7321 atgtattaaa aaagaacagc ccagccacta taagtaccat gaaaagcatt atgcaaatgc
7381 tgctatattt gctgacagca aaaaccaaaa aaccatatgc caacaggctg ttgatactgt
7441 tttagctaaa aagcgggttg atagcctaca attaactaga gaacaaatgt taacaaacag
7501 atttaatgat cttttggata ggatggatat aatgtttggt tctacaggct ctgctgacat
7561 agaagaatgg atggctggag ttgcttggct acactgtttg ttgcccaaaa tggattcagt
7621 ggtgtatgac tttttaaaat gcatggtgta caacattcct aaaaaaagat actggctgtt
7681 taaaggacca attgatagtg gtaaaactac attagcagct gcctcgcgcg tttcggtgat
7741 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
7801 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
7861 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
7921 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
7981 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
8041 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
8101 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
8161 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
8221 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
8281 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
8341 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
8401 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
8461 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
8521 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
8581 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
8641 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
8701 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
8761 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
8821 aaaactcacg ttaagggatt ttggtcatga tgatatattt ttatcttgtg caatgtaaca
8881 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgac
8941 tccccgcgcg cgatgggtcg aatttgcttt cgaaaaaaaa gcccgctcat taggcgggct
9001 aaaaaaaagc ccgctcatta ggcgggctcg aatttctgcc attcatccgc ttattatcac
9061 ttattcaggc gtagcaacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg
9121 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
9181 agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
9241 gcgtataata tttgcccata gtgaaaacgg gggcgaagaa gttgtccata ttcgccacgt
9301 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
9361 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
9421 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
9481 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
9541 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
9601 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
9661 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
9721 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
9781 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
9841 cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt
9901 tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat
9961 cttccgtcac aggtatttat tcgaagacga aagggcatcg cgcgcgggga a
//