LOCUS LT727300 10062 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV71BlahCH3E, complete sequence.
ACCESSION LT727300
VERSION LT727300.1
KEYWORDS .
SOURCE Mammalian expression vector pSV71BlahCH3E
ORGANISM Mammalian expression vector pSV71BlahCH3E
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 10062)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..10062
/organism="Mammalian expression vector pSV71BlahCH3E"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4081."
/db_xref="taxon:1945125"
regulatory 101..382
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 339..414
/note="SV40 ori"
misc_feature 453..698
/note="SV40 large T-antigen"
CDS 453..977
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL88028.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
misc_feature 699..1044
/note="intron"
misc_feature 1045..2925
/note="SV40 large T-antigen"
regulatory complement(2926..3022)
/note="polyA"
/regulatory_class="terminator"
regulatory 2926..3022
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(3219..3257)
/note="E-tag"
CDS complement(<3267..>3584)
/codon_start=1
/transl_table=11
/note="CH3"
/protein_id="SJL88029.1"
/translation="QPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNG
QPENNYNTTPPMLDSDGSFFLYSKLTVDKSRWQQGNIFSCSVMHEALHNRFTQKSLSL
SPGK"
CDS complement(<3599..4457)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88030.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature complement(4571..5508)
/note="intron"
misc_feature complement(5509..5711)
/note="5UTR"
regulatory complement(5712..6007)
/note="SV40 late promoter"
/regulatory_class="promoter"
regulatory 5763..6044
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 6001..6076
/note="SV40 ori"
misc_feature 6115..6360
/note="SV40 large T-antigen"
CDS 6115..6639
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL88031.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
misc_feature 6361..6706
/note="intron"
misc_feature 6707..7769
/note="part of SV40 large t-antigen"
rep_origin complement(8055..8455)
/note="pMB1 ori"
CDS complement(9167..9826)
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL88032.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
BASE COUNT 2897 a 2105 c 2319 g 2741 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
3061 tgtatcttat catgtctgga tccggctcta gagtcgacat tccacagaca gccctcatag
3121 ttagcgtaac gatctaaagt tttgtcgtct ttccagacgt tagtaaatga attttctgta
3181 tgaggttttg ctaaacaact ttcaacagtc tatgcggcac gcggttccag cggatccgga
3241 tacggcaccg gcgcacctgc ggccgcttta cccggagaca gggagaggct cttctgcgtg
3301 aagcggttgt gcagagcctc atgcatcacg gagcatgaga agatgttccc ctgctgccac
3361 ctgctcttgt ccacggtgag cttgctgtag aggaagaagg agccgtcgga gtccagcatg
3421 ggaggcgtgg tgttgtagtt gttctccggc tgcccattgc tctcccactc cacggcgatg
3481 tcgctggggt agaagccttt gaccaggcag gtcaggctga cttggttctt ggtcatctcc
3541 tcccgggatg ggggcagggt gtacacctgt ggttctcggg gctggatccc cgggaattcc
3601 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
3661 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
3721 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
3781 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
3841 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
3901 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
3961 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
4021 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
4081 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
4141 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
4201 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
4261 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
4321 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
4381 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
4441 aaatgttgaa tactcatact cttccttttt caatattatt gaagcagaca gttttattgt
4501 tcatgcctct agaggctgca gccgaccccg gatccggagc ttttagagca gaagtaacac
4561 ttccgtacag gcctagaagt aaaggcaaca tccactgagg agcagttctt tgatttgcac
4621 caccaggagc ctcaaatttt tcaataaatt cacctgactg cacattagga ctttggcttt
4681 gagcttccca cctctcagtt acttgctgaa tactgtctgc ttcatcaata ttatcatagg
4741 tgtgcccaaa tgatatttgc aacccttccc tgttggctac ttgtctcacc attgtaggcc
4801 taatgggaga caaagtagag tagtaatctt gtaaagagtt ataccaatta acaggagcat
4861 taattactgt ccaagtagtt tcctctaaaa accttgccaa actgtccctt aaatatcttt
4921 gggttcttct ttcaagctcc tgtgaggtga gcctaggaat gtcattttgt attacacgcc
4981 aaaaagcttg agaaatggca ttaaaaagtg ttggacccca atgtctgggg tcaagatact
5041 gaacactgtg aacaaaggtt tgtactccag gaaataaaat atcatagtaa tcatctggcc
5101 tatacaaatc tacagccatt cctggttgtt gatataaacc aacagtagaa actttgtgat
5161 cccagtcact aaaaaatcta taccccactt gagcaacagc gctcacacca gtcacagttt
5221 gcagtaaagc tgcaaatcca gctatagcag caggagcccc agatatcaca gcataggcct
5281 gtggagtgag gcctatagca gcaattgcct cagaggttgt taggccttca acagtagcaa
5341 cagatgcaag ttgcacttca attgcagcag cggcctctcc agcagcaatt tcagctactg
5401 aaaatccagt agcagcagca gcttcagaca cagtagcaat taggtccccc aacagtgtta
5461 aagcagcacc catggacctg aaataaaaga caaaaagact aaacttacca gttaactttc
5521 tggtttttca gttaaccttt ctggtttttt gcgtttcccg tcaacagtat cttccccttc
5581 acaaaattgc agcaaaagct ctaaaacaaa cacaaaaagg cgttgagctg tttttttact
5641 ttcagtccat gacctacgaa ccttaacgga ggcctggcgt gacagccggc gcagcaccat
5701 ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc ggaaagaacc
5761 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
5821 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
5881 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
5941 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
6001 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
6061 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc aaagatggat
6121 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
6181 gcctggggga atattcctct gatgagaaag gcatatttaa aaaaatgcaa ggagtttcat
6241 cctgataaag gaggagatga agaaaaaatg aagaaaatga atactctgta caagaaaatg
6301 gaagatggag taaaatatgc tcatcaacct gactttggag gcttctggga tgcaactgag
6361 gtatttgctt cttccttaaa tcctggtgtt gatgcaatgt actgcaaaca atggcctgag
6421 tgtgcaaaga aaatgtctgc taactgcata tgcttgctgt gcttactgag gatgaagcat
6481 gaaaatagaa aattatacag gaaagatcca cttgtgtggg ttgattgcta ctgcttcgat
6541 tgctttagaa tgtggtttgg acttgatctt tgtgaaggaa ccttacttct gtggtgtgac
6601 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
6661 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
6721 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
6781 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
6841 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
6901 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
6961 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
7021 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
7081 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
7141 taaggaatat ttgatgtata gtgccttgac tagagatcca ttttctgtta ttgaggaaag
7201 tttgccaggt gggttaaagg agcatgattt taatccagaa gaagcagagg aaactaaaca
7261 agtgtcctgg aagcttgtaa cagagtatgc aatggaaaca aaatgtgatg atgtgttgtt
7321 attgcttggg atgtacttgg aatttcagta cagttttgaa atgtgtttaa aatgtattaa
7381 aaaagaacag cccagccact ataagtacca tgaaaagcat tatgcaaatg ctgctatatt
7441 tgctgacagc aaaaaccaaa aaaccatatg ccaacaggct gttgatactg ttttagctaa
7501 aaagcgggtt gatagcctac aattaactag agaacaaatg ttaacaaaca gatttaatga
7561 tcttttggat aggatggata taatgtttgg ttctacaggc tctgctgaca tagaagaatg
7621 gatggctgga gttgcttggc tacactgttt gttgcccaaa atggattcag tggtgtatga
7681 ctttttaaaa tgcatggtgt acaacattcc taaaaaaaga tactggctgt ttaaaggacc
7741 aattgatagt ggtaaaacta cattagcagc tgcctcgcgc gtttcggtga tgacggtgaa
7801 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
7861 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
7921 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
7981 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
8041 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
8101 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
8161 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
8221 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
8281 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
8341 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
8401 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
8461 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
8521 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
8581 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
8641 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
8701 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
8761 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
8821 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
8881 gttaagggat tttggtcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
8941 ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga ctccccgcgc
9001 gcgatgggtc gaatttgctt tcgaaaaaaa agcccgctca ttaggcgggc taaaaaaaag
9061 cccgctcatt aggcgggctc gaatttctgc cattcatccg cttattatca cttattcagg
9121 cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct
9181 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
9241 agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
9301 atttgcccat agtgaaaacg ggggcgaaga agttgtccat attcgccacg tttaaatcaa
9361 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
9421 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
9481 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
9541 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
9601 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
9661 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
9721 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
9781 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
9841 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
9901 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg
9961 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca
10021 caggtattta ttcgaagacg aaagggcatc gcgcgcgggg aa
//