LOCUS LT727299 7189 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-TAX1BP1-L, complete sequence.
ACCESSION LT727299
VERSION LT727299.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-TAX1BP1-L
ORGANISM Mammalian expression vector pCAGGS-TAX1BP1-L
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7189)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7189
/organism="Mammalian expression vector pCAGGS-TAX1BP1-L"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4050."
/db_xref="taxon:1944897"
CDS 92..2335
/transl_table=11
/note="hTAX1BP1-L"
/protein_id="SJL88026.1"
/translation="MTSFQEVPLQTSNFAHVIFQNVAKSYLPNAHLECHYTLTPYIHP
HPKDWVGIFKVGWSTARDYYTFLWSPMPEHYVEGSTVNCVLAFQGYYLPNDDGEFYQF
CYVTHKGEIRGASTPFQFRASSPVEELLTMEDEGNSDMLVVTTKAGLLELKIEKTMKE
KEELLKLIAVLEKETAQLREQVGRMERELNHEKERCDQLQAEQKGLTEVTQSLKMENE
EFKKRFSDATSKAHQLEEDIVSVTHKAIEKETELDSLKDKLKKAQHEREQLECQLKTE
KDEKELYKVHLKNTEIENTKLMSEVQTLKNIDGNKESVITHFKEEIGRLQLCLAEKEN
LQRTFLLTTSSKEDTCFLKEQLRKAEEQVQATRQEVVFLAKELSDAVNVRDRTMADLH
TARLENEKVKKQLADAVAELKLNAMKKDQDKTDTLEHELRREVEDLKLRLQMAADHYK
EKFKECQRLQKQINKLSDQSANNNNVFTKKTGNQQKVNDASVNTDPATSASTVDVKPS
PSAAEADFDIVTKGQVCEMTKEIADKTEKYNKCKQLLQDEKAKCNKYADELAKMELKW
KEQVKIAENVKLELAEVQDNYKELKRSLENPAERKMEDGADGAFYPDEIQRPPVRVPS
WGLEDNVVCSQPARNFSRPDGLEDSEDSKEDENVPTAPDPPSQHLRGHGTGFCFDSSF
DVHKKCPLCELMFPPNYDQSKFEEHVESHWKVCPMCSEQFPPDYDQQVFERHVQTHFD
QNVLNFD"
regulatory 2477..2568
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 2569..2925
/note="3' flanking region of rabbit beta-globin"
regulatory complement(3003..3085)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 3227..3302
/note="SV40 ori"
regulatory 3333..3407
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3333..3407)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3522..3923)
/note="pMB1 ori"
CDS complement(4464..5324)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88027.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5464..5841
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5847..7188
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 6216..7140
/note="intron AG promoter"
BASE COUNT 1862 a 1698 c 1861 g 1765 t
ORIGIN
1 aattcnnngg ttcggcggct gatggcggat caggatcgga agcctgcgta actttctccc
61 ttgatccggg agtctttcca ctggattcac aatgacatcc tttcaagaag tcccattgca
121 gacttccaac tttgcccatg tcatctttca aaatgtggcc aagagttacc ttcctaatgc
181 acacctggaa tgtcattaca ccttaactcc atatattcat ccacatccaa aagattgggt
241 tggtatattc aaggttggat ggagtactgc tcgtgattat tacacgtttt tatggtcccc
301 tatgcctgaa cattatgtgg aaggatcaac agtcaattgt gtactagcat tccaaggata
361 ttaccttcca aatgatgatg gagaatttta tcagttctgt tacgttaccc ataagggtga
421 aattcgtgga gcaagtacac ctttccagtt tcgagcttct tctccagttg aagagctgct
481 tactatggaa gatgaaggaa attctgacat gttagtggtg accacaaaag caggccttct
541 tgagttgaaa attgagaaaa ccatgaaaga aaaagaagaa ctgttaaagt taattgccgt
601 tctggaaaaa gaaacagcac aacttcgaga acaagttggg agaatggaaa gagaacttaa
661 ccatgagaaa gaaagatgtg accaactgca agcagaacaa aagggtctta ctgaagtaac
721 acaaagctta aaaatggaaa atgaagagtt taagaagagg ttcagtgatg ctacatccaa
781 agcccatcag cttgaggaag atattgtgtc agtaacacat aaagcaattg aaaaagaaac
841 cgaattagac agtttaaagg acaaactcaa gaaggcacaa catgaaagag aacaacttga
901 atgtcagttg aagacagaga aggatgaaaa ggaactttat aaggtacatt tgaagaatac
961 agaaatagaa aataccaagc ttatgtcaga ggtccagact ttaaaaaata tagatgggaa
1021 caaagaaagc gtgattactc atttcaaaga agagattggc aggctgcagt tatgtttggc
1081 tgaaaaggaa aatctgcaaa gaactttcct gcttacaacc tcaagtaaag aagatacttg
1141 ttttttaaag gagcaacttc gtaaagcaga ggaacaggtt caggcaactc ggcaagaagt
1201 tgtctttctg gctaaagaac tcagtgatgc tgtcaacgta cgagacagaa cgatggcaga
1261 cctgcatact gcacgcttgg aaaacgagaa agtgaaaaag cagttagctg atgcagtggc
1321 agaacttaaa ctaaatgcta tgaaaaaaga tcaggacaag actgatacac tggaacacga
1381 actaagaaga gaagttgaag atctgaaact ccgtcttcag atggctgcag accattataa
1441 agaaaaattt aaggaatgcc aaaggctcca aaaacaaata aacaaacttt cagatcaatc
1501 agctaataat aataatgtct tcacaaagaa aacggggaat cagcagaaag tgaatgatgc
1561 ttcagtaaac acagacccag ccacttctgc ctctactgta gatgtaaagc catcaccttc
1621 tgcagcagag gcagattttg acatagtaac aaaggggcaa gtctgtgaaa tgaccaaaga
1681 aattgctgac aaaacagaaa agtataataa atgtaaacaa ctcttgcagg atgagaaagc
1741 aaaatgcaat aaatatgctg atgaacttgc aaaaatggag ctgaaatgga aagaacaagt
1801 gaaaattgct gaaaatgtaa aacttgaact agctgaagta caggacaatt ataaagaact
1861 taaaaggagt ctagaaaatc cagcagaaag gaaaatggaa gatggagcag atggtgcttt
1921 ttacccagat gaaatacaaa ggccacctgt cagagtcccc tcttggggac tggaagacaa
1981 tgttgtctgc agccagcctg ctcgaaactt tagtcggcct gatggcttag aggactctga
2041 ggatagcaaa gaagatgaga atgtgcctac tgctcctgat cctccaagtc aacatttacg
2101 tgggcatggg acaggctttt gctttgattc cagctttgat gttcacaaga agtgtcccct
2161 ctgtgagtta atgtttcctc ctaactatga tcagagcaaa tttgaagaac atgttgaaag
2221 tcactggaag gtgtgcccga tgtgcagcga gcagttccct cctgactatg accagcaggt
2281 gtttgaaagg catgtgcaga cccattttga tcagaatgtt ctaaattttg actagttact
2341 ttttattatg agttaatata gtttagcagt aaaaaaaaaa aaaaaaaaac tcgaggaatt
2401 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
2461 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
2521 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
2581 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
2641 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
2701 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
2761 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
2821 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
2881 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
2941 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
3001 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag
3061 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
3121 ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
3181 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
3241 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
3301 ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt
3361 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
3421 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg
3481 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
3541 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
3601 ggtaatacgg ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc
3661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
3721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
3781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
3841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
3901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
3961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
4021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
4081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
4141 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
4201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
4261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
4321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
4381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
4441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
4501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
4561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
4621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
4681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
4741 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
4801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
4861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
4921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
4981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
5041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
5101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
5161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
5221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
5281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
5341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
5401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg
5461 atcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
5521 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
5581 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
5641 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
5701 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
5761 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
5821 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
5881 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
5941 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc
6001 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
6061 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
6121 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc
6181 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
6241 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
6301 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
6361 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
6421 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
6481 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
6541 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
6601 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
6661 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
6721 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc
6781 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
6841 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
6901 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
6961 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
7021 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
7081 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
7141 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaag
//