LOCUS LT727296 7417 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pPHT, complete sequence.
ACCESSION LT727296
VERSION LT727296.1
KEYWORDS .
SOURCE Vector pPHT
ORGANISM Vector pPHT
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7417)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7417
/organism="Vector pPHT"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3960."
/db_xref="taxon:1945361"
regulatory complement(402..853)
/note="3' UTR of mPGK1, incl. polyA"
/regulatory_class="terminator"
regulatory complement(1393..1462)
/note="HSV-TK polyA"
/regulatory_class="terminator"
CDS complement(1463..2593)
/transl_table=11
/note="HSV-TK"
/protein_id="SJL88018.1"
/translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQ
KMPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYT
TQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSNAPPPALTLI
FDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLA
KRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWWEDWGQLSGTAVPPQGAEPQS
NAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPA
GCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
regulatory complement(2691..3198)
/note="mPGK1 promoter"
/regulatory_class="promoter"
regulatory complement(3236..3514)
/note="3' UTR of mPGK1, incl. polyA"
/regulatory_class="terminator"
CDS complement(3518..4543)
/transl_table=11
/note="hygromycin resistance"
/protein_id="SJL88019.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE"
regulatory complement(4553..5060)
/note="mPGK1 promoter"
/regulatory_class="promoter"
regulatory complement(5170..5186)
/note="T3 promoter"
/regulatory_class="promoter"
regulatory complement(5250..5333)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(5425..5816)
/note="ColE1 ori"
CDS complement(6357..7217)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88020.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1688 a 2049 c 2072 g 1608 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctgatg gaattagaac
421 ttggcaaaac aatactgaga atgaagtgta tgtggaacag aggctgctga tctcgttctt
481 caggctatga aactgacaca tttggaaacc acagtactta gaaccacaaa gtgggaatca
541 agagaaaaac aatgatccca cgagagatct atagatctat agatcatgag tgggaggaat
601 gagctggccc ttaatttggt tttgcttgtt taaattatga tatccaacta tgaaacatta
661 tcataaagca atagtaaaga gccttcagta aagagcaggc atttatctaa tcccacccca
721 cccccacccc cgtagctcca atccttccat tcaaaatgta ggtactctgt tctcaccctt
781 cttaacaaag tatgacagga aaaacttcca ttttagtgga catctttatt gtttaataga
841 tcatcaattt ctgcaggtcc tggccggggt cccgcggaaa ctcggccgtg gtgaccaata
901 caaaacaaaa gcgctcctcg taccagcgaa gaaggggcag agatgccgta gtcaggttta
961 gttcgtccgg cggcgccaga aatccgcgcg gtggtttttg ggggtcgggg gtgtttggca
1021 gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg gtccatgccc aggccatcca
1081 aaaaccatgg gtctgtctgc tcagtccagt cgtggacctg accccacgca acgcccaaaa
1141 gaataacccc cacgaaccat aaaccattcc ccatggggga ccccgtccct aacccacggg
1201 gcccgtggct atggcgggct tgccgccccg acgttggctg cgagccctgg gccttcaccc
1261 gaacttgggg gttggggtgg ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt
1321 ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac
1381 gacccaacac ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
1441 cggtattgtc tccttccgtg tttcagttag cctcccccat ctcccgggca aacgtgcgcg
1501 ccaggtcgca gatcgtcggt atggagcctg gggtggtgac gtgggtctgg accatcccgg
1561 aggtaagttg cagcagggcg tcccggcagc cggcgggcga ttggtcgtaa tccaggataa
1621 agacgtgcat gggacggagg cgtttggcca agacgtccaa ggcccaggca aacacgttat
1681 acaggtcgcc gttgggggcc agcaactcgg gggcccgaaa cagggtaaat aacgtgtccc
1741 cgatatgggg tcgtgggccc gcgttgctct ggggctcggc accctggggc ggcacggccg
1801 tccccgaaag ctgtccccaa tcctcccacc acgacccgcc gccctgcaga taccgcaccg
1861 tattggcaag cagcccgtaa acgcggcgaa tcgcggccag catagccagg tcaagccgct
1921 cgccggggcg ctggcgtttg gccaggcggt cgatgtgtct gtcctccgga agggccccca
1981 acacgatgtt tgtgccgggc aaggtcggcg ggatgagggc cacgaacgcc agcacggcct
2041 ggggggtcat gctgcccata aggtatcgcg cggccgggta gcacaggagg gcggcgatgg
2101 gatggcggtc gaagatgagg gtgagggccg ggggcggggc atttgagctc ccagcctccc
2161 ccccgatatg aggagccaga acggcgtcgg tcacggcata aggcatgccc attgttatct
2221 gggcgcttgt cattaccacc gccgcgtccc cggccgatat ctcaccctgg tcgaggcggt
2281 gttgtgtggt gtagatgttc gcgattgtct cggaagcccc cagcacctgc cagtaagtca
2341 tcggctcggg tacgtagacg atatcgtcgc gcgaacccag ggccaccagc agttgcgtgg
2401 tggtggtttt ccccatcccg tgaggaccgt ctatataaac ccgcagtagc gtgggcattt
2461 tctgctccag gcggacttcc gtggcttctt gctgccggcg agggcgcaac gccgtacgtc
2521 ggttgctatg gccgcgagaa cgcgcagcct ggtcgaacgc agacgcgtgt tgatggcagg
2581 ggtacgaagc catacgcgct tctacaaggc gcttgccaaa gaggtgcggg agtttcacgc
2641 caccaagatc tgcggcacgc tgttgacgct gttaagcggg tcgctgcagg tcgaaaggcc
2701 cggagatgag gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc
2761 cgggcctccg gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc
2821 cccggaccca ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg
2881 gccgctgccc caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag
2941 actagtgaga cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg
3001 ggggaacttc ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg
3061 agccggttgg cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc cacttgtgta
3121 gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc
3181 ctcccctacc cggtagaatt cgagctcggt acccggggat cctctagaac tagtggatct
3241 atagatcatg agtgggagga atgagctggc ccttaatttg gttttgcttg tttaaattat
3301 gatatccaac tatgaaacat tatcataaag caatagtaaa gagccttcag taaagagcag
3361 gcatttatct aatcccaccc cacccccacc cccgtagctc caatccttcc attcaaaatg
3421 taggtactct gttctcaccc ttcttaacaa agtatgacag gaaaaacttc cattttagtg
3481 gacatcttta ttgtttaata gatcatcaat ttctcgacta ttcctttgcc ctcggacgag
3541 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
3601 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
3661 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
3721 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct
3781 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
3841 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
3901 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
3961 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
4021 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
4081 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
4141 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga
4201 ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
4261 cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg
4321 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
4381 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
4441 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
4501 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catggtggcg ggtcgaaagg
4561 cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat
4621 gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc cctgagcccg
4681 cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca aagctgctat
4741 tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc catctgcacg
4801 agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg
4861 ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc accaaagaac
4921 ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg
4981 tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc
5041 gcctccccta cccggtagaa ttcatcgatg atatcagatc ccccgggctg caggaattcg
5101 atatcaagct tatcgatacc gtcgacctcg agcggccgcc accgcggtgg agctccagct
5161 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
5221 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
5281 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
5341 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
5401 ggagaggcgg tttgcgtatt gggcgctttt ccgcttcctc gctcactgac tcgctacgct
5461 cggtcgttcg actgcggcga gcggtactga ctcacacaaa aacggtaaca cagttatcca
5521 cagaatcagg ggataaggcc ggaaagaaca tgtgagcaaa agaccaggaa caggaagaag
5581 gccacgtagc aggcgttttt ccataggctc cgcccccctg acgagcatca caaaaataga
5641 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
5701 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
5761 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
5821 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
5881 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
5941 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
6001 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
6061 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
6121 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
6181 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
6241 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
6301 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
6361 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
6421 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
6481 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
6541 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
6601 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
6661 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
6721 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
6781 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
6841 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
6901 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
6961 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
7021 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
7081 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
7141 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
7201 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
7261 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
7321 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
7381 acctataaaa ataggcgtat cacgaggccc tttcgtc
//