LOCUS LT727295 6871 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAGmA20f6, complete sequence.
ACCESSION LT727295
VERSION LT727295.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAGmA20f6
ORGANISM Mammalian expression vector pCAGGS-FLAGmA20f6
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6871)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6871
/organism="Mammalian expression vector pCAGGS-FLAGmA20f6"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3957."
/db_xref="taxon:1944858"
misc_feature 13..36
/note="FLAG epitope"
CDS 46..>2142
/transl_table=11
/note="mA20 (1-699)"
/protein_id="SJL88016.1"
/translation="MAEQLLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIYHFKTMH
RYTLEMFRTCQFCPQFREIIHKALIDRSVQASLESQKKLNWCREVRKLVALKTNGDGN
CLMHAACQYMWGVQDTDLVLRKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYD
TRNWNDEWDNLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDKMLRSL
ESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPELRAVPLV
NRDRGRFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDHGTTHLINAAKLDEAN
LPKEINLVDDYFELVQHEYKKWQENSDQARRAAHAQNPLEPSTPQLSLMDIKCETPNC
PFFMSVNTQPLCHECSERRQKNQSKLPKLNSKLGPEGLPGVGLGSSNWSPEETAGGPH
SAPPTAPSLFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHARQINASHTADPGKCQA
CLQDVTRTFNGICSTCFKRTTAEPSSSLTSSIPASCHQRSKSDPSQLIQSLTPHSCHR
TGNVSPSGCLSQAARTPGDRAGTSKCRKAGCMYFGTPENKGFCTLCFIEYRENKQSVT
ASAKAGSPAPRFQNNVPCLGRECGTLGSTMFEGYCQKCFIEAQNQRFHEARRTEEQLR
SSQHRDMPRTTQVASRL"
regulatory 2157..2248
/note="3' UTR of beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 2249..2605
/note="3' flanking region of rabbit beta-globin"
regulatory complement(2683..2765)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2907..2982
/note="SV40 ori"
regulatory 3013..3087
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3013..3087)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3202..3603)
/note="pMB1 ori"
CDS complement(4144..5004)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88017.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5144..5521
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5527..6868
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5896..6820
/note="intron AG promoter"
BASE COUNT 1586 a 1816 c 1829 g 1640 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt
61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc
121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga
181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac
241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg
301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg
361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc
421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa
481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat
541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga
601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga
661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct
721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga
781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac
841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac
901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag
961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc
1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac
1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga
1141 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata
1201 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac
1261 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta
1321 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc
1381 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc
1441 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga
1501 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag
1561 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc
1621 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc ctcctgtcac
1681 caacgctcca agtctgaccc ctcacaactc atccaaagtc tcactccaca ctcttgccac
1741 cggactggaa atgtctctcc ttctggctgc ctctcccagg ctgcacggac tccaggagac
1801 agagcaggga caagcaagtg caggaaagct ggctgcatgt attttgggac tccagaaaac
1861 aagggctttt gcactctatg tttcatcgaa tacagagaaa ataagcagtc tgttactgcc
1921 tctgcgaaag ctggttcccc ggcccccagg ttccagaaca atgtcccgtg cctgggcagg
1981 gagtgcggca cactcggaag caccatgttt gaagggtact gtcagaagtg tttcatcgaa
2041 gctcagaacc agagattcca tgaagcaaga agaacggaag aacagctgag atcaagccag
2101 catagagaca tgcctcgaac tacacaggta gcctcaaggc tgtaagtgtc gacgaagatc
2161 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
2221 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
2281 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
2341 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
2401 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
2461 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
2521 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
2581 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg
2641 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
2701 ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt
2761 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa
2821 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
2881 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
2941 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
3001 ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
3061 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
3121 atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga
3181 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
3241 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
3301 aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
3361 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
3421 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
3481 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
3541 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
3601 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
3661 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
3721 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
3781 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
3841 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
3901 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
3961 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
4021 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
4081 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
4141 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
4201 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
4261 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
4321 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
4381 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
4441 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
4501 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
4561 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
4621 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
4681 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
4741 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
4801 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
4861 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
4921 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
4981 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
5041 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
5101 ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg atcgacattg attattgact
5161 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
5221 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
5281 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
5341 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
5401 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
5461 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
5521 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
5581 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
5641 ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa
5701 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
5761 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc
5821 cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
5881 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
5941 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
6001 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
6061 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
6121 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
6181 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
6241 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
6301 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
6361 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
6421 gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
6481 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
6541 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
6601 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
6661 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
6721 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
6781 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
6841 tattgtgctg tctcatcatt ttggcaaaga a
//