LOCUS LT727294 6544 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-FLAGmA20f5, complete sequence. ACCESSION LT727294 VERSION LT727294.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-FLAGmA20f5 ORGANISM Mammalian expression vector pCAGGS-FLAGmA20f5 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6544) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6544 /organism="Mammalian expression vector pCAGGS-FLAGmA20f5" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 3956." /db_xref="taxon:1944857" misc_feature 13..36 /note="FLAG epitope" CDS 46..>1815 /transl_table=11 /note="mA20 (1-590)" /protein_id="SJL88014.1" /translation="MAEQLLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIYHFKTMH RYTLEMFRTCQFCPQFREIIHKALIDRSVQASLESQKKLNWCREVRKLVALKTNGDGN CLMHAACQYMWGVQDTDLVLRKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYD TRNWNDEWDNLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDKMLRSL ESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPELRAVPLV NRDRGRFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDHGTTHLINAAKLDEAN LPKEINLVDDYFELVQHEYKKWQENSDQARRAAHAQNPLEPSTPQLSLMDIKCETPNC PFFMSVNTQPLCHECSERRQKNQSKLPKLNSKLGPEGLPGVGLGSSNWSPEETAGGPH SAPPTAPSLFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHARQINASHTADPGKCQA CLQDVTRTFNGICSTCFKRTTAEPSSSLTSSIPASCHQRSKSDPSQLIQSLTPHSCHR TGNVSPSGCLSQAARTPGDRAGTS" regulatory 1830..1921 /note="3' UTR of beta-globin, incl. polyA" /regulatory_class="terminator" misc_feature 1922..2278 /note="3' flanking region of rabbit beta-globin" regulatory complement(2356..2438) /note="lac promoter" /regulatory_class="promoter" rep_origin 2580..2655 /note="SV40 ori" regulatory 2686..2760 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(2686..2760) /note="SV40 polyA late" /regulatory_class="terminator" rep_origin complement(2875..3276) /note="pMB1 ori" CDS complement(3817..4677) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL88015.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 4817..5194 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 5200..6541 /note="AG promoter" /regulatory_class="promoter" misc_feature 5569..6493 /note="intron AG promoter" BASE COUNT 1490 a 1738 c 1742 g 1574 t ORIGIN 1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt 61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc 121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga 181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac 241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg 301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg 361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc 421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa 481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat 541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga 601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga 661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct 721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga 781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac 841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac 901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag 961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc 1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac 1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga 1141 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata 1201 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac 1261 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta 1321 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc 1381 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc 1441 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga 1501 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag 1561 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc 1621 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc ctcctgtcac 1681 caacgctcca agtctgaccc ctcacaactc atccaaagtc tcactccaca ctcttgccac 1741 cggactggaa atgtctctcc ttctggctgc ctctcccagg ctgcacggac tccaggagac 1801 agagcaggga caagctaagt gtcgacgaag atctttttcc ctctgccaaa aattatgggg 1861 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg 1921 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc 1981 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg 2041 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg 2101 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt 2161 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat 2221 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc 2281 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 2341 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg 2401 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 2461 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct 2521 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 2581 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 2641 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca 2701 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 2761 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 2821 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 2881 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 2941 ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat 3001 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 3061 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 3121 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 3181 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 3241 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 3301 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 3361 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 3421 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 3481 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 3541 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 3601 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 3661 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 3721 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 3781 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 3841 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 3901 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 3961 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 4021 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 4081 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 4141 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 4201 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 4261 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 4321 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 4381 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 4441 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 4501 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 4561 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 4621 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 4681 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 4741 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 4801 gccacctggg tcgatcgaca ttgattattg actagttatt aatagtaatc aattacgggg 4861 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 4921 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 4981 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 5041 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 5101 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 5161 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc 5221 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa 5281 ttattttgtg cagcgatggg ggcggggggg gggggggggc cccccccagg cggggcgggg 5341 cggggcgagg ggcggggcgg ggcgaggcgg aaaggtgcgg cggcagccaa tcagagcggc 5401 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga 5461 agcgcgcggc gggcgggagt cgttgcgcgc tgccttcccc ccgtgccccg ctccgccgcc 5521 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg 5581 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt 5641 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc 5701 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc 5761 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag 5821 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc 5881 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg 5941 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg 6001 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg 6061 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaagg ggcgcggcgg 6121 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta 6181 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg 6241 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa 6301 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca 6361 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt 6421 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt 6481 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa 6541 agaa //