LOCUS LT727293 4805 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS2, complete sequence.
ACCESSION LT727293
VERSION LT727293.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS2
ORGANISM Mammalian expression vector pCAGGS2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4805)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4805
/organism="Mammalian expression vector pCAGGS2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3955."
/db_xref="taxon:1944754"
regulatory 91..182
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 183..539
/note="3' flanking region of rabbit beta-globin"
regulatory complement(617..699)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 841..916
/note="SV40 ori"
regulatory 947..1021
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(947..1021)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(1136..1537)
/note="pMB1 ori"
CDS complement(2078..2938)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88013.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3078..3455
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3461..4802
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 3830..4754
/note="intron AG promoter"
BASE COUNT 1004 a 1273 c 1338 g 1190 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
121 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
181 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
241 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
301 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
361 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
421 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
481 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
541 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
601 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggt
661 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
721 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc
781 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
841 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
901 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc
961 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
1021 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
1081 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
1141 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
1201 gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca ggaaagaaca
1261 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
1321 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
1381 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
1441 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
1501 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
1561 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
1621 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
1681 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
1741 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
1801 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
1861 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
1921 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
1981 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
2041 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
2101 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
2161 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
2221 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
2281 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
2341 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
2401 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
2461 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
2521 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
2581 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
2641 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
2701 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
2761 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
2821 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
2881 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
2941 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
3001 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
3061 tgccacctgg gtcgatcgac attgattatt gactagttat taatagtaat caattacggg
3121 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
3181 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
3241 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
3301 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
3361 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
3421 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
3481 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
3541 attattttgt gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg
3601 gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg
3661 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
3721 aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc
3781 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
3841 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
3901 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
3961 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
4021 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
4081 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
4141 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
4201 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
4261 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
4321 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg
4381 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
4441 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
4501 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
4561 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
4621 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
4681 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
4741 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
4801 aagaa
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