LOCUS LT727292 7099 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAGmA20, complete sequence.
ACCESSION LT727292
VERSION LT727292.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAGmA20
ORGANISM Mammalian expression vector pCAGGS-FLAGmA20
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7099)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7099
/organism="Mammalian expression vector pCAGGS-FLAGmA20"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3954."
/db_xref="taxon:1944852"
misc_feature 13..36
/note="FLAG epitope"
CDS 46..2373
/transl_table=11
/note="mA20"
/protein_id="SJL88011.1"
/translation="MAEQLLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIYHFKTMH
RYTLEMFRTCQFCPQFREIIHKALIDRSVQASLESQKKLNWCREVRKLVALKTNGDGN
CLMHAACQYMWGVQDTDLVLRKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYD
TRNWNDEWDNLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDKMLRSL
ESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPELRAVPLV
NRDRGRFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDHGTTHLINAAKLDEAN
LPKEINLVDDYFELVQHEYKKWQENSDQARRAAHAQNPLEPSTPQLSLMDIKCETPNC
PFFMSVNTQPLCHECSERRQKNQSKLPKLNSKLGPEGLPGVGLGSSNWSPEETAGGPH
SAPPTAPSLFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHARQINASHTADPGKCQA
CLQDVTRTFNGICSTCFKRTTAEPSSSLTSSIPASCHQRSKSDPSQLIQSLTPHSCHR
TGNVSPSGCLSQAARTPGDRAGTSKCRKAGCMYFGTPENKGFCTLCFIEYRENKQSVT
ASAKAGSPAPRFQNNVPCLGRECGTLGSTMFEGYCQKCFIEAQNQRFHEARRTEEQLR
SSQHRDMPRTTQVASRPKCARASCKNILACRSEELCMECQHLSQRVGSVAHRGEPTPE
EPPKQRCRAPACDHFGNAKCNGYCNECYQFKQMYG"
regulatory 2385..2476
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 2477..2833
/note="3' FR of beta-globin"
regulatory complement(2911..2993)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 3135..3210
/note="SV40 ori"
regulatory 3241..3315
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3241..3315)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3430..3831)
/note="pMB1 ori"
CDS complement(4372..5232)
/transl_table=11
/note="ampicillin"
/protein_id="SJL88012.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5372..5749
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5755..7096
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 6124..7048
/note="intron AG promoter"
BASE COUNT 1637 a 1881 c 1894 g 1687 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt
61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc
121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga
181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac
241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg
301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg
361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc
421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa
481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat
541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga
601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga
661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct
721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga
781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac
841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac
901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag
961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc
1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac
1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga
1141 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata
1201 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac
1261 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta
1321 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc
1381 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc
1441 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga
1501 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag
1561 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc
1621 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc ctcctgtcac
1681 caacgctcca agtctgaccc ctcacaactc atccaaagtc tcactccaca ctcttgccac
1741 cggactggaa atgtctctcc ttctggctgc ctctcccagg ctgcacggac tccaggagac
1801 agagcaggga caagcaagtg caggaaagct ggctgcatgt attttgggac tccagaaaac
1861 aagggctttt gcactctatg tttcatcgaa tacagagaaa ataagcagtc tgttactgcc
1921 tctgcgaaag ctggttcccc ggcccccagg ttccagaaca atgtcccgtg cctgggcagg
1981 gagtgcggca cactcggaag caccatgttt gaagggtact gtcagaagtg tttcatcgaa
2041 gctcagaacc agagattcca tgaagcaaga agaacggaag aacagctgag atcaagccag
2101 catagagaca tgcctcgaac tacacaggta gcctcaaggc cgaaatgtgc ccgggcctcc
2161 tgcaagaaca ttctggcctg tcgcagtgag gaactctgta tggagtgcca gcacctaagc
2221 caacgagtag gttctgtggc ccaccggggt gagcccacgc ctgaagagcc ccctaaacag
2281 cgctgccggg cccctgcttg tgatcacttt ggcaatgcca agtgtaatgg ttactgcaat
2341 gagtgctacc agttcaagca gatgtatggc taagtgtcga cgaagatctt tttccctctg
2401 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
2461 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
2521 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
2581 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
2641 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
2701 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
2761 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
2821 tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt
2881 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
2941 tgtaaagcct gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
3001 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac
3061 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
3121 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
3181 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
3241 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
3301 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
3361 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
3421 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
3481 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcagggataa
3541 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
3601 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
3661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
3721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
3781 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
3841 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
3901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
3961 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
4021 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
4081 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
4141 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
4201 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
4261 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
4321 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
4381 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
4441 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
4501 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
4561 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
4621 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
4681 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
4741 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
4801 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
4861 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
4921 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
4981 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
5041 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
5101 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
5161 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
5221 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
5281 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
5341 atttccccga aaagtgccac ctgggtcgat cgacattgat tattgactag ttattaatag
5401 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
5461 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
5521 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
5581 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
5641 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
5701 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
5761 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
5821 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcccccc
5881 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggaaagg tgcggcggca
5941 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
6001 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgttg cgcgctgcct tccccccgtg
6061 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
6121 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
6181 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg
6241 tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc
6301 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
6361 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc
6421 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc
6481 gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg
6541 gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg
6601 tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg
6661 gaaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
6721 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc
6781 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg
6841 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc
6901 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg
6961 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
7021 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
7081 tcatcatttt ggcaaagaa
//