LOCUS LT727290 4056 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pBlue-mA20f3, complete sequence.
ACCESSION LT727290
VERSION LT727290.1
KEYWORDS .
SOURCE Vector pBlue-mA20f3
ORGANISM Vector pBlue-mA20f3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4056)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4056
/organism="Vector pBlue-mA20f3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3952."
/db_xref="taxon:1945255"
rep_origin 4..459
/note="f1 ori"
regulatory 626..642
/note="T7 promoter"
/regulatory_class="promoter"
CDS complement(746..1846)
/transl_table=11
/note="mA20 (1-366)"
/protein_id="SJL88007.1"
/translation="MAEQLLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIYHFKTMH
RYTLEMFRTCQFCPQFREIIHKALIDRSVQASLESQKKLNWCREVRKLVALKTNGDGN
CLMHAACQYMWGVQDTDLVLRKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYD
TRNWNDEWDNLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDKMLRSL
ESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPELRAVPLV
NRDRGRFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDHGTTHLINAAKLDEAN
LPKEINLVDDYFELVQHEYKKWQENSDQARRA"
regulatory complement(1870..1886)
/note="T3 promoter"
/regulatory_class="promoter"
misc_feature complement(1912..1949)
/note="lacZ RBS"
regulatory complement(1950..2033)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(2125..2527)
/note="pMB1 ori"
CDS complement(3068..3928)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88008.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 966 a 1021 c 983 g 1086 t
ORIGIN
1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
661 ccgcggtggc ggccgctcta gaactagtgg atcgatcccc cgggctgcag gaattcgata
721 tcaagcttat cgataccgtc gacacttacg ctctcctggc ctgatcgctg ttctcctgcc
781 atttcttgta ttcgtgctga acaagctcaa agtaatcgtc taccaaattt atttctttgg
841 gtaagttagc ttcatccaat tttgcagcgt tgatcaggtg agtcgtgccg tggtcccagc
901 cttgcacagg gatctccatc actatcaagt actcctttag aagcttttcc ttcatctcat
961 tctcaggatc tgtcaagaag tgaactttta agtcttcaaa cctaccccgg tctctgttaa
1021 caagtggaac agcgcgaagt tcaggtccac tgtccttcag ggtcaccagg ggtacaaagt
1081 gctggctgtc atagcctagg acgatgggat atctgtaaca ctcctgggca ggccagtgaa
1141 gaggcagata aatcccaccc actttcaaag gagcaaaatt ggaaccagat tccaaacttc
1201 ttagcatttt gtctgaaatg acaatgatgg gtcttctgag gatgttgctg aggacaaata
1261 tgtggatttc ttccagggaa ttgtactgaa gtccacttcg ggctgcaggt gtgtctgctg
1321 atgccatttt gaccaagttg tcccattcgt cattccagtt ccgagtgtcg tagcaaagtc
1381 ctgtttccac aaattcctga gatttcagag attccagctg ccagcggaat ttaaagttcc
1441 gagtgtctgt ctccttaagg gtgctgcaga gggccttcct caggaccagg tcagtatcct
1501 gaacacccca catgtactga caagctgcat gcatgaggca gtttccatca ccattggttt
1561 tcagagccac gagcttcctg acttcacgac accagttgag cttcttctgg ctttccaggg
1621 aagcctggac acttctgtca ataagtgctt tgtggatgat ctctcggaac tgtgggcaaa
1681 actggcatgt tctgaacatc tccagcgtgt atcggtgcat ggttttaaag tgatagatga
1741 tcccattggt aggtttgaaa atgtcttctg gggttctctc tcgtatcttc acagctttcc
1801 gcatattgct caaatacaaa gcctgaggaa gaagttgttc agccatggcg gtacccagct
1861 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
1921 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
1981 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
2041 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
2101 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
2161 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
2221 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
2281 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
2341 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
2401 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
2461 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
2521 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
2581 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
2641 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
2701 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
2761 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
2821 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
2881 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
2941 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
3001 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
3061 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
3121 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
3181 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
3241 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
3301 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
3361 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
3421 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
3481 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
3541 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
3601 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
3661 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
3721 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
3781 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
3841 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
3901 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
3961 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
4021 taggggttcc gcgcacattt ccccgaaaag tgccac
//