LOCUS LT727289 9568 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pPP1IVHAsi, complete sequence.
ACCESSION LT727289
VERSION LT727289.1
KEYWORDS .
SOURCE Yeast expression vector pPP1IVHAsi
ORGANISM Yeast expression vector pPP1IVHAsi
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9568)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9568
/organism="Yeast expression vector pPP1IVHAsi"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3924."
/db_xref="taxon:1945493"
regulatory 7..772
/note="AOX1"
/regulatory_class="promoter"
misc_feature 773..937
/note="5UTR"
misc_feature 949..1191
/note="ppMF"
CDS <1216..>2763
/codon_start=1
/transl_table=11
/note="IVHAs"
/protein_id="SJL88004.1"
/translation="QDLPGNDNNSTATLCLGHHAVPNGTLVKTITNDQIEVTNATELV
QSSSTGKICNNPHRILDGINCTLIDALLGDPHCDGFQNEKWDLFVERSKAFSNCYPYD
VPDYASLRSLVASSGTLEFINEGFNWTGVTQNGGSSACKRGPDSGFFSRLNWLYKSGS
TYPVQNVTMPNNDNSDKLYIWGVHHPSTDKEQTNLYVQASGKVTVSTKRSQQTIIPNV
GSRPWVRGLSSRISIYWTIVKPGDILVINSNGNLIAPRGYFKMRTGKSSIMRSDAPIG
TCSSECITPNGSIPNDKPFQNVNKITYGACPKYVKQNTLKLATGMRNVPEKQTRGIFG
AIAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEK
FHQIEKEFSEVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFE
KTRRQLRENAEDMGNGCFKIYHKCDNACIGSIRNGTYDHDVYRDEALNNRFQIKGVEL
KSGYKDWI"
regulatory 2854..3113
/note="AOX1T"
/regulatory_class="terminator"
CDS complement(3513..6047)
/transl_table=11
/note="HIS4"
/protein_id="SJL88005.1"
/translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQST
ELQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYR
TLVDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGII
PIIDARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSS
KKSIPEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGF
CHLERTSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAE
AKSKEDIAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQ
PKEESKPKEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQ
NGDKALLELTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLT
ETLQVETCPGVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASP
PKKDGTLTPEVIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTA
AKMMVQNDTSALCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAV
DMTDKELARIEDAVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLI
LQIENASSYVDQVQHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQ
KFITSQDVTPEGLKHIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
misc_feature 6414..7171
/note="3FR"
rep_origin complement(7455..7857)
/note="pMB1 ori"
CDS complement(8398..9258)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88006.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2668 a 2216 c 2172 g 2512 t
ORIGIN
1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct
961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact
1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta
1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg
1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa
1201 agagaggctg aagcccaaga ccttccagga aatgacaaca acagcacagc aacgctgtgc
1261 ctgggacatc atgcggtgcc aaacggaacg ctagtgaaaa caatcacgaa tgatcagatt
1321 gaagtgacta atgctactga gctggttcag agttcctcaa cgggtaaaat atgcaacaat
1381 cctcatcgaa tccttgatgg aataaactgc acactgatag atgctctatt gggggaccct
1441 cattgtgatg gatttcaaaa tgagaaatgg gaccttttcg ttgaacgcag caaagctttc
1501 agcaactgtt acccttatga tgtgccagat tatgcctccc ttaggtcact agttgcctcg
1561 tcaggcactc tggagtttat caatgaaggc ttcaattgga ctggggtcac tcagaatggg
1621 ggaagcagtg cttgcaaaag aggacctgat agcggttttt tcagtagact gaactggttg
1681 tacaaatcag gaagcacata tccagtgcaa aacgtgacca tgccaaacaa tgacaattct
1741 gacaaactat acatttgggg ggttcaccac ccgagcacgg acaaagaaca aaccaaccta
1801 tatgttcaag catcagggaa agtcacagtc tccaccaaga gaagccagca aactataatc
1861 ccgaatgtcg ggtctagacc ctgggtaagg ggtctgtcta gtagaataag catctattgg
1921 acaatagtta aaccgggaga catactggta attaatagta atgggaacct aattgctcct
1981 cggggttact tcaaaatgcg cactgggaaa agctcaataa tgaggtcaga tgcacctatt
2041 ggcacctgca gctctgaatg catcactcca aatggaagca ttcccaatga caagcccttt
2101 caaaacgtaa acaagatcac atatggggca tgtcccaagt atgttaagca aaacactctg
2161 aagttggcaa cagggatgcg gaatgtacca gagaaacaaa ctagaggcat attcggcgca
2221 atagcaggtt tcatagaaaa tggttgggag ggaatgatag acggttggta cggtttcagg
2281 catcaaaatt ccgagggcac aggacaagca gcagatctta aaagcactca agcagccatc
2341 gaccaaatca atgggaaact gaatagggta atcgagaaga cgaacgagaa attccatcaa
2401 atcgaaaagg aattctcaga agtagaaggg agaattcagg acctcgagaa atacgttgaa
2461 gacactaaaa tagatctctg gtcttacaat gcggagcttc ttgtcgctct ggagaaccaa
2521 catacaattg atctgactga ctcggaaatg aacaaactgt ttgaaaaaac aaggaggcaa
2581 ctgagggaaa atgctgagga catgggcaat ggttgcttca aaatatacca caaatgtgac
2641 aatgcttgca tagggtcaat cagaaatggg acttatgacc atgatgtata cagagacgaa
2701 gcattaaaca accggtttca gatcaaaggt gttgaactga agtcaggata caaagactgg
2761 atctaagcgg ccgcgaatta attcgcctta gacatgactg ttcctcagtt caagttgggc
2821 acttacgaga agaccggtct tgctagattc taatcaagag gatgtcagaa tgccatttgc
2881 ctgagagatg caggcttcat ttttgatact tttttatttg taacctatat agtataggat
2941 tttttttgtc attttgtttc ttctcgtacg agcttgctcc tgatcagcct atctcgcagc
3001 tgatgaatat cttgtggtag gggtttggga aaatcattcg agtttgatgt ttttcttggt
3061 atttcccact cctcttcaga gtacagaaga ttaagtgaga agttcgtttg tgcaagctta
3121 tcgataagct ttaatgcggt agtttatcac agttaaattg ctaacgcagt caggcaccgt
3181 gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag
3241 gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc cattccgaca
3301 gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa tttctatgcg
3361 cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc
3421 tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg tggatctatc
3481 gaatctaaat gtaagttaaa atctctaaat aattaaataa gtcccagttt ctccatacga
3541 accttaacag cattgcggtg agcatctaga ccttcaacag cagccagatc catcactgct
3601 tggccaatat gtttcagtcc ctcaggagtt acgtcttgtg aagtgatgaa cttctggaag
3661 gttgcagtgt taactccgct gtattgacgg gcatatccgt acgttggcaa agtgtggttg
3721 gtaccggagg agtaatctcc acaactctct ggagagtagg caccaacaaa cacagatcca
3781 gcgtgttgta cttgatcaac ataagaagaa gcattctcga tttgcaggat caagtgttca
3841 ggagcgtact gattggacat ttccaaagcc tgctcgtagg ttgcaaccga tagggttgta
3901 gagtgtgcaa tacacttgcg tacaatttca acccttggca actgcacagc ttggttgtga
3961 acagcatctt caattctggc aagctccttg tctgtcatat cgacagccaa cagaatcacc
4021 tgggaatcaa taccatgttc agcttgagac agaaggtctg aggcaacgaa atctggatca
4081 gcgtatttat cagcaataac tagaacttca gaaggcccag caggcatgtc aatactacac
4141 agggctgatg tgtcattttg aaccatcatc ttggcagcag taacgaactg gtttcctgga
4201 ccaaatattt tgtcacactt aggaacagtt tctgttccgt aagccatagc agctactgcc
4261 tgggcgcctc ctgctagcac gatacactta gcaccaacct tgtgggcaac gtagatgact
4321 tctggggtaa gggtaccatc cttcttaggt ggagatgcaa aaacaatttc tttgcaacca
4381 gcaactttgg caggaacacc cagcatcagg gaagtggaag gcagaattgc ggttccacca
4441 ggaatataga ggccaacttt ctcaataggt cttgcaaaac gagagcagac tacaccaggg
4501 caagtctcaa cttgcaacgt ctccgttagt tgagcttcat ggaatttcct gacgttatct
4561 atagagagat caatggctct cttaacgtta tctggcaatt gcataagttc ctctgggaaa
4621 ggagcttcta acacaggtgt cttcaaagcg actccatcaa acttggcagt tagttctaaa
4681 agggctttgt caccattttg acgaacattg tcgacaattg gtttgactaa ttccataatc
4741 tgttccgttt tctggatagg acgacgaagg gcatcttcaa tttcttgtga ggaggcctta
4801 gaaacgtcaa ttttgcacaa ttcaatacga ccttcagaag ggacttcttt aggtttggat
4861 tcttctttag gttgttcctt ggtgtatcct ggcttggcat ctcctttcct tctagtgacc
4921 tttagggact tcatatccag gtttctctcc acctcgtcca acgtcacacc gtacttggca
4981 catctaacta atgcaaaata aaataagtca gcacattccc aggctatatc ttccttggat
5041 ttagcttctg caagttcatc agcttcctcc ctaattttag cgttcaacaa aacttcgtcg
5101 tcaaataacc gtttggtata agaaccttct ggagcattgc tcttacgatc ccacaaggtg
5161 gcttccatgg ctctaagacc ctttgattgg ccaaaacagg aagtgcgttc caagtgacag
5221 aaaccaacac ctgtttgttc aaccacaaat ttcaagcagt ctccatcaca atccaattcg
5281 atacccagca acttttgagt tgctccagat gtagcacctt tataccacaa accgtgacga
5341 cgagattggt agactccagt ttgtgtcctt atagcctccg gaatagactt tttggacgag
5401 tacaccaggc ccaacgagta attagaagag tcagccacca aagtagtgaa tagaccatcg
5461 gggcggtcag tagtcaaaga cgccaacaaa atttcactga cagggaactt tttgacatct
5521 tcagaaagtt cgtattcagt agtcaattgc cgagcatcaa taatggggat tataccagaa
5581 gcaacagtgg aagtcacatc taccaacttt gcggtctcag aaaaagcata aacagttcta
5641 ctaccgccat tagtgaaact tttcaaatcg cccagtggag aagaaaaagg cacagcgata
5701 ctagcattag cgggcaagga tgcaacttta tcaaccaggg tcctatagat aaccctagcg
5761 cctgggatca tcctttggac aactctttct gccaaatcta ggtccaaaat cacttcattg
5821 ataccattat tgtacaactt gagcaagttg tcgatcagct cctcaaattg gtcctctgta
5881 acggatgact caacttgcac attaacttga agctcagtcg attgagtgaa cttgatcagg
5941 ttgtgcagct ggtcagcagc atagggaaac acggcttttc ctaccaaact caaggaatta
6001 tcaaactctg caacacttgc gtatgcaggt agcaagggaa atgtcatact tgaagtcgga
6061 cagtgagtgt agtcttgaga aattctgaag ccgtattttt attatcagtg agtcagtcat
6121 caggagatcc tctacgccgg acgcatcgtg gccgacctgc aggtcggcat caccggcgcc
6181 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
6241 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
6301 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
6361 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagta
6421 tctatgattg gaagtatggg aatggtgata cccgcattct tcagtgtctt gaggtctcct
6481 atcagattat gcccaactaa agcaaccgga ggaggagatt tcatggtaaa tttctctgac
6541 ttttggtcat cagtagactc gaactgtgag actatctcgg ttatgacagc agaaatgtcc
6601 ttcttggaga cagtaaatga agtcccacca ataaagaaat ccttgttatc aggaacaaac
6661 ttcttgtttc gaactttttc ggtgccttga actataaaat gtagagtgga tatgtcgggt
6721 aggaatggag cgggcaaatg cttaccttct ggaccttcaa gaggtatgta gggtttgtag
6781 atactgatgc caacttcagt gacaacgttg ctatttcgtt caaaccattc cgaatccaga
6841 gaaatcaaag ttgtttgtct actattgatc caagccagtg cggtcttgaa actgacaata
6901 gtgtgctcgt gttttgaggt catctttgta tgaataaatc tagtctttga tctaaataat
6961 cttgacgagc caaggcgata aatacccaaa tctaaaactc ttttaaaacg ttaaaaggac
7021 aagtatgtct gcctgtatta aaccccaaat cagctcgtag tctgatcctc atcaacttga
7081 ggggcactat cttgttttag agaaatttgc ggagatgcga tatcgagaaa aaggtacgct
7141 gattttaaac gtgaaattta tctcaagatc tctgcctcgc gcgtttcggt gatgacggtg
7201 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
7261 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
7321 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
7381 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
7441 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
7501 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
7561 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
7621 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
7681 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
7741 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
7801 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
7861 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
7921 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
7981 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
8041 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
8101 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
8161 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
8221 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
8281 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
8341 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
8401 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
8461 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
8521 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
8581 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
8641 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
8701 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
8761 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
8821 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
8881 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
8941 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
9001 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
9061 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
9121 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
9181 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
9241 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
9301 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
9361 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
9421 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattaa ttctcatgtt
9481 tgacagctta tcatcgataa gctgactcat gttggtattg tgaaatagac gcagatcggg
9541 aacactgaaa aataacagtt attattcg
//