LOCUS LT727288 4288 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pUCIVHAsm, complete sequence.
ACCESSION LT727288
VERSION LT727288.1
KEYWORDS .
SOURCE Vector pUCIVHAsm
ORGANISM Vector pUCIVHAsm
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4288)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4288
/organism="Vector pUCIVHAsm"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3923."
/db_xref="taxon:1945431"
misc_feature 440..487
/note="sIVHA"
CDS <488..>2035
/codon_start=1
/transl_table=11
/note="IVHAs"
/protein_id="SJL88002.1"
/translation="QDLPGNDNNSTATLCLGHHAVPNGTLVKTITNDQIEVTNATELV
QSSSTGKICNNPHRILDGINCTLIDALLGDPHCDGFQNEKWDLFVERSKAFSNCYPYD
VPDYASLRSLVASSGTLEFINEGFNWTGVTQNGGSSACKRGPDSGFFSRLNWLYKSGS
TYPVQNVTMPNNDNSDKLYIWGVHHPSTDKEQTNLYVQASGKVTVSTKRSQQTIIPNV
GSRPWVRGLSSRISIYWTIVKPGDILVINSNGNLIAPRGYFKMRTGKSSIMRSDAPIG
TCSSECITPNGSIPNDKPFQNVNKITYGACPKYVKQNTLKLATGMRNVPEKQTRGIFG
AIAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEK
FHQIEKEFSEVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFE
KTRRQLRENAEDMGNGCFKIYHKCDNACIGSIRNGTYDHDVYRDEALNNRFQIKGVEL
KSGYKDWI"
regulatory complement(2110..2193)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(2285..2687)
/note="pMB1 ori"
CDS complement(3228..4088)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88003.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1203 a 1005 c 1060 g 1020 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
421 accgataatt ctattaacca tgaagactat cattgctttg agctacattt tctgtctggt
481 ttgggcccaa gaccttccag gaaatgacaa caacagcaca gcaacgctgt gcctgggaca
541 tcatgcggtg ccaaacggaa cgctagtgaa aacaatcacg aatgatcaga ttgaagtgac
601 taatgctact gagctggttc agagttcctc aacgggtaaa atatgcaaca atcctcatcg
661 aatccttgat ggaataaact gcacactgat agatgctcta ttgggggacc ctcattgtga
721 tggatttcaa aatgagaaat gggacctttt cgttgaacgc agcaaagctt tcagcaactg
781 ttacccttat gatgtgccag attatgcctc ccttaggtca ctagttgcct cgtcaggcac
841 tctggagttt atcaatgaag gcttcaattg gactggggtc actcagaatg ggggaagcag
901 tgcttgcaaa agaggacctg atagcggttt tttcagtaga ctgaactggt tgtacaaatc
961 aggaagcaca tatccagtgc aaaacgtgac catgccaaac aatgacaatt ctgacaaact
1021 atacatttgg ggggttcacc acccgagcac ggacaaagaa caaaccaacc tatatgttca
1081 agcatcaggg aaagtcacag tctccaccaa gagaagccag caaactataa tcccgaatgt
1141 cgggtctaga ccctgggtaa ggggtctgtc tagtagaata agcatctatt ggacaatagt
1201 taaaccggga gacatactgg taattaatag taatgggaac ctaattgctc ctcggggtta
1261 cttcaaaatg cgcactggga aaagctcaat aatgaggtca gatgcaccta ttggcacctg
1321 cagctctgaa tgcatcactc caaatggaag cattcccaat gacaagccct ttcaaaacgt
1381 aaacaagatc acatatgggg catgtcccaa gtatgttaag caaaacactc tgaagttggc
1441 aacagggatg cggaatgtac cagagaaaca aactagaggc atattcggcg caatagcagg
1501 tttcatagaa aatggttggg agggaatgat agacggttgg tacggtttca ggcatcaaaa
1561 ttccgagggc acaggacaag cagcagatct taaaagcact caagcagcca tcgaccaaat
1621 caatgggaaa ctgaataggg taatcgagaa gacgaacgag aaattccatc aaatcgaaaa
1681 ggaattctca gaagtagaag ggagaattca ggacctcgag aaatacgttg aagacactaa
1741 aatagatctc tggtcttaca atgcggagct tcttgtcgct ctggagaacc aacatacaat
1801 tgatctgact gactcggaaa tgaacaaact gtttgaaaaa acaaggaggc aactgaggga
1861 aaatgctgag gacatgggca atggttgctt caaaatatac cacaaatgtg acaatgcttg
1921 catagggtca atcagaaatg ggacttatga ccatgatgta tacagagacg aagcattaaa
1981 caaccggttt cagatcaaag gtgttgaact gaagtcagga tacaaagact ggatccccgg
2041 gtaccgagct cgaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
2101 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
2161 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
2221 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
2281 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
2341 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
2401 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
2461 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
2521 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
2581 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
2641 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
2701 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
2761 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
2821 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
2881 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
2941 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
3001 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
3061 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
3121 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
3181 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
3241 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
3301 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
3361 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
3421 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
3481 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
3541 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
3601 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
3661 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
3721 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
3781 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
3841 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
3901 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
3961 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
4021 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
4081 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
4141 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
4201 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
4261 aataggcgta tcacgaggcc ctttcgtc
//