LOCUS       LT727287                5032 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Vector pLTp35, complete sequence.
ACCESSION   LT727287
VERSION     LT727287.1
KEYWORDS    .
SOURCE      Vector pLTp35
  ORGANISM  Vector pLTp35
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Vector pLTp35"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 3918."
                     /db_xref="taxon:1945333"
     regulatory      24..40
                     /note="T7"
                     /regulatory_class="promoter"
     misc_feature    41..103
                     /note="5UTR"
     misc_feature    113..145
                     /note="H6EK"
     CDS             155..1054
                     /transl_table=11
                     /note="p35"
                     /protein_id="SJL87999.1"
                     /translation="MCVIFPVEIDVSQTIIRDCQVDKQTRELVYINKIMNTQLTKPVL
                     MMFNISGPIRSVTRKNNNLRDRIKSKVDEQFDQLERDYSDQMDGFHDSIKYFKDEHYS
                     VSCQNGSVLKSKFAKILKSHDYTDKKSIEAYEKYCLPKLVDERNDYYVAVCVLKPGFE
                     NGSNQVLSFEYNPIGNKVIVPFAHEINDTGLYEYDVVAYVDSVQFDGEQFEEFVQSLI
                     LPSSFKNSEKVLYYNEASKNKSMIYKALEFTTESSWGKSEKYNWKIFCNGFIYDKKSK
                     VLYVKLHNVTSALNKNVILNTIK"
     CDS             <1099..1497
                     /codon_start=1
                     /transl_table=11
                     /note="fragment of the human IL2 CDS"
                     /protein_id="SJL88000.1"
                     /translation="PTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFY
                     MPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTF
                     MCEYADETATIVEFLNRWITFCQSIISTLT"
     regulatory      complement(1637..1644)
                     /note="T3"
                     /regulatory_class="promoter"
     regulatory      1661..1708
                     /note="T7T"
                     /regulatory_class="terminator"
     regulatory      1782..1829
                     /note="T7T"
                     /regulatory_class="terminator"
     regulatory      1898..1979
                     /note="fdT"
                     /regulatory_class="terminator"
     regulatory      2008..2089
                     /note="fdT"
                     /regulatory_class="terminator"
     rep_origin      2187..2642
                     /note="f1 ori"
     rep_origin      complement(2646..3019)
                     /note="pMB1 ori"
     CDS             complement(3560..4420)
                     /transl_table=11
                     /note="ampicillin resistance"
                     /protein_id="SJL88001.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      4786..4916
                     /note="PL"
                     /regulatory_class="promoter"
BASE COUNT         1434 a         1143 c         1129 g         1325 t
ORIGIN      
        1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc
       61 ctctagaaat aattttgttt aactttaaga aggagatata catatggatc cacatcacca
      121 tcaccatcac gacgatgacg ataaggcgat ccctatgtgt gtaatttttc cggtagaaat
      181 cgacgtgtcc cagacgatta ttcgagattg tcaggtggac aaacaaacca gagagttggt
      241 gtacattaac aagattatga acacgcaatt gacaaaaccc gttctcatga tgtttaacat
      301 ttcgggtcct atacgaagcg ttacgcgcaa gaacaacaat ttgcgcgaca gaataaaatc
      361 aaaagtcgat gaacaatttg atcaactaga acgcgattac agcgatcaaa tggatggatt
      421 ccacgatagc atcaagtatt ttaaagatga acactattcg gtaagttgcc aaaatggcag
      481 cgtgttgaaa agcaagtttg ctaaaatttt aaagagtcat gattataccg ataaaaagtc
      541 tattgaagct tacgagaaat actgtttgcc caaattggtc gacgaacgca acgactacta
      601 cgtggcggta tgcgtgttga agccgggatt tgagaacggc agcaaccaag tgctatcttt
      661 cgagtacaac ccgattggta acaaagttat tgtgccgttt gctcacgaaa ttaacgacac
      721 gggactttac gagtacgacg tcgtagctta cgtggacagt gtgcagtttg atggcgaaca
      781 atttgaagag tttgtgcaga gtttaatatt gccgtcgtcg ttcaaaaatt cggaaaaggt
      841 tttatattac aacgaagcgt cgaaaaacaa aagcatgatc tacaaggctt tagagtttac
      901 tacagaatcg agctggggca aatccgaaaa gtataattgg aaaatttttt gtaacggttt
      961 tatttatgat aaaaaatcaa aagtgttgta tgttaaattg cacaatgtaa ctagtgcact
     1021 caacaaaaat gtaatattaa acacaattaa ataaatgtta aaatttattg cctaatatta
     1081 ttttgtcatt gcgaattgcc tacttcaagt tctacaaaga aaacacagct acaactggag
     1141 catttactgc tggatttaca gatgattttg aatggaatta ataattacaa gaatcccaaa
     1201 ctcaccagga tgctcacatt taagttttac atgcccaaga aggccacaga actgaaacat
     1261 cttcagtgtc tagaagaaga actcaaacct ctggaggaag tgctaaattt agctcaaagc
     1321 aaaaactttc acttaagacc cagggactta atcagcaata tcaacgtaat agttctggaa
     1381 ctaaagggat ctgaaacaac attcatgtgt gaatatgctg atgagacagc aaccattgta
     1441 gaatttctga acagatggat taccttttgt caaagcatca tctcaacact gacttgataa
     1501 ttaagtgctt cccacttaaa acatatcagg gggttcgaaa tcgataagct tatgcatgcg
     1561 gccgcatcta gagggcccgg atccctcgag gtcgacgaat tcgagctcgg ccgacttggc
     1621 cttcccttta gtgagggtta ataaacttgg tgagcaataa ctagcataac cccttggggc
     1681 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatgcg ctcatacgat
     1741 atgaacgttg agactgccgc tgagttatca gtnagcaata actagcataa ccccttgggg
     1801 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatagctta
     1861 tcgctagagg tcgaaattca cctcgaaagc aagctgataa accgatacaa ttaaaggctc
     1921 cttttggagc cttttttttt ggagattttc aacgtgaaaa aattattatt cgcaattcca
     1981 agctaattca cctcgaaagc aagctgataa accgatacaa ttaaaggctc cttttggagc
     2041 cttttttttt ggagattttc aacgtgaaaa aattattatt cgcaattcca agctctgcct
     2101 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     2161 agcttgtctg taagcggatg cagatcacgc gccctgtagc ggcgcattaa gcgcggcggg
     2221 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2281 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2341 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2401 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2461 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     2521 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     2581 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     2641 ttgatctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2701 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2761 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2821 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2881 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2941 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     3001 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3061 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3121 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3181 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3241 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3301 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3361 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3421 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3481 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3541 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3601 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3661 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3721 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3781 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3841 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     3901 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3961 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4021 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4081 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4141 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     4201 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4261 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     4321 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4381 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4441 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat
     4501 cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag
     4561 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa
     4621 atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg
     4681 ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga
     4741 cctcagcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctct cacctaccaa
     4801 acaatgcccc cctgcaaaaa ataaattcat ataaaaaaca tacagataac catctgcggt
     4861 gataaattat ctctggcggt gttgacataa ataccactgg cggtgatact gagcacatca
     4921 gcaggacgca ctgaccacca tgaaggtgac gctcttaaaa ttaagccctg aagaagggca
     4981 gcattcaaag cagaaggctt tggggtgtgt gatacgaaac gaagcattgg aa
//