LOCUS LT727287 5032 bp DNA circular SYN 06-FEB-2017 DEFINITION Vector pLTp35, complete sequence. ACCESSION LT727287 VERSION LT727287.1 KEYWORDS . SOURCE Vector pLTp35 ORGANISM Vector pLTp35 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5032) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5032 /organism="Vector pLTp35" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 3918." /db_xref="taxon:1945333" regulatory 24..40 /note="T7" /regulatory_class="promoter" misc_feature 41..103 /note="5UTR" misc_feature 113..145 /note="H6EK" CDS 155..1054 /transl_table=11 /note="p35" /protein_id="SJL87999.1" /translation="MCVIFPVEIDVSQTIIRDCQVDKQTRELVYINKIMNTQLTKPVL MMFNISGPIRSVTRKNNNLRDRIKSKVDEQFDQLERDYSDQMDGFHDSIKYFKDEHYS VSCQNGSVLKSKFAKILKSHDYTDKKSIEAYEKYCLPKLVDERNDYYVAVCVLKPGFE NGSNQVLSFEYNPIGNKVIVPFAHEINDTGLYEYDVVAYVDSVQFDGEQFEEFVQSLI LPSSFKNSEKVLYYNEASKNKSMIYKALEFTTESSWGKSEKYNWKIFCNGFIYDKKSK VLYVKLHNVTSALNKNVILNTIK" CDS <1099..1497 /codon_start=1 /transl_table=11 /note="fragment of the human IL2 CDS" /protein_id="SJL88000.1" /translation="PTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFY MPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTF MCEYADETATIVEFLNRWITFCQSIISTLT" regulatory complement(1637..1644) /note="T3" /regulatory_class="promoter" regulatory 1661..1708 /note="T7T" /regulatory_class="terminator" regulatory 1782..1829 /note="T7T" /regulatory_class="terminator" regulatory 1898..1979 /note="fdT" /regulatory_class="terminator" regulatory 2008..2089 /note="fdT" /regulatory_class="terminator" rep_origin 2187..2642 /note="f1 ori" rep_origin complement(2646..3019) /note="pMB1 ori" CDS complement(3560..4420) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL88001.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 4786..4916 /note="PL" /regulatory_class="promoter" BASE COUNT 1434 a 1143 c 1129 g 1325 t ORIGIN 1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc 61 ctctagaaat aattttgttt aactttaaga aggagatata catatggatc cacatcacca 121 tcaccatcac gacgatgacg ataaggcgat ccctatgtgt gtaatttttc cggtagaaat 181 cgacgtgtcc cagacgatta ttcgagattg tcaggtggac aaacaaacca gagagttggt 241 gtacattaac aagattatga acacgcaatt gacaaaaccc gttctcatga tgtttaacat 301 ttcgggtcct atacgaagcg ttacgcgcaa gaacaacaat ttgcgcgaca gaataaaatc 361 aaaagtcgat gaacaatttg atcaactaga acgcgattac agcgatcaaa tggatggatt 421 ccacgatagc atcaagtatt ttaaagatga acactattcg gtaagttgcc aaaatggcag 481 cgtgttgaaa agcaagtttg ctaaaatttt aaagagtcat gattataccg ataaaaagtc 541 tattgaagct tacgagaaat actgtttgcc caaattggtc gacgaacgca acgactacta 601 cgtggcggta tgcgtgttga agccgggatt tgagaacggc agcaaccaag tgctatcttt 661 cgagtacaac ccgattggta acaaagttat tgtgccgttt gctcacgaaa ttaacgacac 721 gggactttac gagtacgacg tcgtagctta cgtggacagt gtgcagtttg atggcgaaca 781 atttgaagag tttgtgcaga gtttaatatt gccgtcgtcg ttcaaaaatt cggaaaaggt 841 tttatattac aacgaagcgt cgaaaaacaa aagcatgatc tacaaggctt tagagtttac 901 tacagaatcg agctggggca aatccgaaaa gtataattgg aaaatttttt gtaacggttt 961 tatttatgat aaaaaatcaa aagtgttgta tgttaaattg cacaatgtaa ctagtgcact 1021 caacaaaaat gtaatattaa acacaattaa ataaatgtta aaatttattg cctaatatta 1081 ttttgtcatt gcgaattgcc tacttcaagt tctacaaaga aaacacagct acaactggag 1141 catttactgc tggatttaca gatgattttg aatggaatta ataattacaa gaatcccaaa 1201 ctcaccagga tgctcacatt taagttttac atgcccaaga aggccacaga actgaaacat 1261 cttcagtgtc tagaagaaga actcaaacct ctggaggaag tgctaaattt agctcaaagc 1321 aaaaactttc acttaagacc cagggactta atcagcaata tcaacgtaat agttctggaa 1381 ctaaagggat ctgaaacaac attcatgtgt gaatatgctg atgagacagc aaccattgta 1441 gaatttctga acagatggat taccttttgt caaagcatca tctcaacact gacttgataa 1501 ttaagtgctt cccacttaaa acatatcagg gggttcgaaa tcgataagct tatgcatgcg 1561 gccgcatcta gagggcccgg atccctcgag gtcgacgaat tcgagctcgg ccgacttggc 1621 cttcccttta gtgagggtta ataaacttgg tgagcaataa ctagcataac cccttggggc 1681 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatgcg ctcatacgat 1741 atgaacgttg agactgccgc tgagttatca gtnagcaata actagcataa ccccttgggg 1801 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatagctta 1861 tcgctagagg tcgaaattca cctcgaaagc aagctgataa accgatacaa ttaaaggctc 1921 cttttggagc cttttttttt ggagattttc aacgtgaaaa aattattatt cgcaattcca 1981 agctaattca cctcgaaagc aagctgataa accgatacaa ttaaaggctc cttttggagc 2041 cttttttttt ggagattttc aacgtgaaaa aattattatt cgcaattcca agctctgcct 2101 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 2161 agcttgtctg taagcggatg cagatcacgc gccctgtagc ggcgcattaa gcgcggcggg 2221 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 2281 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 2341 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 2401 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 2461 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 2521 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 2581 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat 2641 ttgatctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 2701 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 2761 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 2821 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 2881 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 2941 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct 3001 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 3061 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 3121 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 3181 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 3241 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 3301 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 3361 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 3421 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 3481 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 3541 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 3601 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 3661 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 3721 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 3781 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 3841 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt 3901 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 3961 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 4021 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 4081 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 4141 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata 4201 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 4261 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 4321 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 4381 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 4441 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat 4501 cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag 4561 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa 4621 atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg 4681 ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga 4741 cctcagcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctct cacctaccaa 4801 acaatgcccc cctgcaaaaa ataaattcat ataaaaaaca tacagataac catctgcggt 4861 gataaattat ctctggcggt gttgacataa ataccactgg cggtgatact gagcacatca 4921 gcaggacgca ctgaccacca tgaaggtgac gctcttaaaa ttaagccctg aagaagggca 4981 gcattcaaag cagaaggctt tggggtgtgt gatacgaaac gaagcattgg aa //