LOCUS LT727283 6295 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMxCATlinkDD, complete sequence.
ACCESSION LT727283
VERSION LT727283.1
KEYWORDS .
SOURCE Vector pMxCATlinkDD
ORGANISM Vector pMxCATlinkDD
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6295)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6295
/organism="Vector pMxCATlinkDD"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3908."
/db_xref="taxon:1945358"
regulatory 13..458
/note="Mx"
/regulatory_class="promoter"
CDS 515..>1171
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL87989.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
misc_feature 1172..1225
/note="G4S"
CDS <1229..1534
/codon_start=1
/transl_table=11
/note="DD"
/protein_id="SJL87990.1"
/translation="DPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLRE
AQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR"
misc_feature 1535..1685
/note="3UTR"
CDS <1741..1815
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL87991.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 1816..1882
/note="intron of SV40 small t-antigen"
misc_feature 1883..2434
/note="3' UTR of SV40 small t-antigen"
regulatory 2435..2531
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2435..2531)
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(2532..3344)
/note="SV40 VP1 with internal deletion"
regulatory complement(3400..3483)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(3575..3977)
/note="pMB1 ori"
CDS complement(4518..5378)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87992.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 6273..6289
/note="SP6"
/regulatory_class="promoter"
BASE COUNT 1609 a 1525 c 1539 g 1622 t
ORIGIN
1 aagcttgggc tgcagtagca gtgctcagac ctctaaacct gtagtcacaa gggacccaga
61 agggctggca gggctcctca gtgattctgg tatggtttgt ctctgaccct tcctagtgca
121 gctggaagaa tccagtgaaa agagaaagtt agtctaggtt gtgttcaact gagaggaggt
181 caggctgaag gctcagcaca gagctggaag ggtcctggga aagcaggcag ccatctgaac
241 tggtgtccat actgcatgtg tcttggtggg ctgagcctac aatatcgatt tcccaacctc
301 agtaccaagc caaggcccct cagggtgagt ttcgtttctg agctcctttc atatgtggtg
361 ggggtgtggg cacagcaagc tcaggctttt tcacggtttc aattctcctc tggaaggggc
421 ggagccagca ctatttaaag aggtctgagc cctggcagga ctctagagga tctcgaggag
481 cttggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
541 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
601 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
661 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
721 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
781 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
841 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
901 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
961 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
1021 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
1081 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
1141 acagtactgc gatgagtggc agggcggggc gggcggatcc ggtggaggcg gttcaggcgg
1201 aggtggctct ggcggtggcg gatccatgga ccccgcgacg ctgtacgccg tggtggagaa
1261 cgtgcccccg ttgcgctgga aggaattcgt gcggcgccta gggctgagcg accacgagat
1321 cgatcggctg gagctgcaga acgggcgctg cctgcgcgag gcgcaataca gcatgctggc
1381 gacctggagg cggcgcacgc cgcggcgcga ggccacgctg gagctgctgg gacgcgtgct
1441 ccgcgacatg gacctgctgg gctgcctgga ggacatcgag gaggcgcttt gcggccccgc
1501 cgccctcccg cccgcgccca gtcttctcag atgaggctgc gcccctgcgg gcagctctaa
1561 ggaccgtcct gcgagatcgc cttccaaccc cacttttttc tggaaaggag gggtcctgca
1621 ggggcaagca ggagctagca gccgcctact tggtgctaac ccctcgatgt acatagcttt
1681 tctcaggggg atccccccta ggcttttgca aaaagctcaa gcttggctct agagtcgacg
1741 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
1801 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
1861 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
1921 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga
1981 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc
2041 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac
2101 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat
2161 tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc ataacatact
2221 gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt
2281 gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc
2341 cttgactaga gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
2401 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
2461 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
2521 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
2581 atgtctggat cgatccccag gaagctcctc tgtgtcctca taaaccctaa cctcctctac
2641 ttgagaggac attccaatca taggctgccc atccaccctc tgtgtcctcc tgttaattag
2701 gtcacttaac aaaaaggaaa ttgggtaggg gtttttcaca gaccgctttc taagggtaat
2761 tttaaaatat ctgggaagtc ccttccactg ctgtgttcca gaagtgttgg taaacagccc
2821 acaaatgtca acagcagaaa catacaagct gtcagctttg cacaagggcc caacaccctg
2881 ctcatcaaga agcactgtgg ttgctgtgtt agtaatgtgc aaaacaggag gcacattttc
2941 cccacctgtg taggttccaa aatatctagt gttttcattt ttacttggat caggaaccca
3001 gcactccact ggataagcat tatccttatc caaaacagcc ttgtggtcag tgttcatctg
3061 ctgactgtca actgtagcat tttttggggt tacagtttga gcaggatatt tggtcctgta
3121 gtttgctaac acaccctgca gctccaaagg ttccccacca acagcaaaaa aatgaaaatt
3181 tgacccttga atgggttttc cagcaccatt ttcatgagtt ttttgtgtcc ctgaatgcaa
3241 gtttaacata gcagttaccc caataacctc agttttaaca gtaacagctt cccacatcaa
3301 aatatttcca caggttaagt cctcatttaa attaggcaaa ggaattcgta atcatggtca
3361 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
3421 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
3481 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
3541 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
3601 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
3661 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
3721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
3781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
3841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
3901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
3961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
4021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
4081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
4141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
4201 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
4261 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
4321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
4381 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
4441 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
4501 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
4561 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
4621 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
4681 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
4741 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
4801 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
4861 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
4921 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
4981 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
5041 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
5101 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
5161 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
5221 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
5281 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
5341 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
5401 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
5461 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
5521 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
5581 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
5641 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
5701 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
5761 catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
5821 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
5881 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
5941 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
6001 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta gcgatgaccc
6061 tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga aactcagaag
6121 gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag ggtctgcttc
6181 agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat
6241 taatacataa ccttatgtat catacacata cgatttaggt gacactatag aatac
//