LOCUS LT727282 6250 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMxCAThTNFDD, complete sequence.
ACCESSION LT727282
VERSION LT727282.1
KEYWORDS .
SOURCE Vector pMxCAThTNFDD
ORGANISM Vector pMxCAThTNFDD
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6250)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6250
/organism="Vector pMxCAThTNFDD"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3907."
/db_xref="taxon:1945356"
regulatory 13..458
/note="Mx"
/regulatory_class="promoter"
CDS 515..>1171
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL87985.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
misc_feature 1172..1180
/note="GGS"
CDS <1184..1489
/codon_start=1
/transl_table=11
/note="DD"
/protein_id="SJL87986.1"
/translation="DPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLRE
AQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR"
misc_feature 1490..1641
/note="3UTR"
CDS <1696..1770
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL87987.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 1771..1837
/note="intron of SV40 small t-antigen"
misc_feature 1838..2389
/note="3' UTR of SV40 small t-antigen"
regulatory 2390..2486
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2390..2486)
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(2487..3299)
/note="SV40 VP1 with internal deletion"
regulatory complement(3355..3438)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(3530..3930)
/note="pMB1 ori"
CDS complement(4473..5333)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87988.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 6228..6244
/note="SP6"
/regulatory_class="promoter"
BASE COUNT 1605 a 1516 c 1515 g 1614 t
ORIGIN
1 aagcttgggc tgcagtagca gtgctcagac ctctaaacct gtagtcacaa gggacccaga
61 agggctggca gggctcctca gtgattctgg tatggtttgt ctctgaccct tcctagtgca
121 gctggaagaa tccagtgaaa agagaaagtt agtctaggtt gtgttcaact gagaggaggt
181 caggctgaag gctcagcaca gagctggaag ggtcctggga aagcaggcag ccatctgaac
241 tggtgtccat actgcatgtg tcttggtggg ctgagcctac aatatcgatt tcccaacctc
301 agtaccaagc caaggcccct cagggtgagt ttcgtttctg agctcctttc atatgtggtg
361 ggggtgtggg cacagcaagc tcaggctttt tcacggtttc aattctcctc tggaaggggc
421 ggagccagca ctatttaaag aggtctgagc cctggcagga ctctagagga tctcgaggag
481 cttggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
541 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
601 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
661 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
721 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
781 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
841 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
901 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
961 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
1021 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
1081 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
1141 acagtactgc gatgagtggc agggcggggc gggcggatcc atggaccccg cgacgctgta
1201 cgccgtggtg gagaacgtgc ccccgttgcg ctggaaggaa ttcgtgcggc gcctagggct
1261 gagcgaccac gagatcgatc ggctggagct gcagaacggg cgctgcctgc gcgaggcgca
1321 atacagcatg ctggcgacct ggaggcggcg cacgccgcgg cgcgaggcca cgctggagct
1381 gctgggacgc gtgctccgcg acatggacct gctgggctgc ctggaggaca tcgaggaggc
1441 gctttgcggc cccgccgccc tcccgcccgc gcccagtctt ctcagatgag gctgcgcccc
1501 tgcgggcagc tctaaggacc gtcctgcgag atcgccttcc aaccccactt ttttctggaa
1561 aggaggggtc ctgcaggggc aagcaggagc tagcagccgc ctacttggtg ctaacccctc
1621 gatgtacata gcttttctca gggggatccc ccctaggctt ttgcaaaaag ctcaagcttg
1681 gctctagagt cgacggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
1741 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
1801 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
1861 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
1921 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
1981 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
2041 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
2101 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
2161 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
2221 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
2281 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
2341 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
2401 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
2461 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
2521 tcaatgtatc ttatcatgtc tggatcgatc cccaggaagc tcctctgtgt cctcataaac
2581 cctaacctcc tctacttgag aggacattcc aatcataggc tgcccatcca ccctctgtgt
2641 cctcctgtta attaggtcac ttaacaaaaa ggaaattggg taggggtttt tcacagaccg
2701 ctttctaagg gtaattttaa aatatctggg aagtcccttc cactgctgtg ttccagaagt
2761 gttggtaaac agcccacaaa tgtcaacagc agaaacatac aagctgtcag ctttgcacaa
2821 gggcccaaca ccctgctcat caagaagcac tgtggttgct gtgttagtaa tgtgcaaaac
2881 aggaggcaca ttttccccac ctgtgtaggt tccaaaatat ctagtgtttt catttttact
2941 tggatcagga acccagcact ccactggata agcattatcc ttatccaaaa cagccttgtg
3001 gtcagtgttc atctgctgac tgtcaactgt agcatttttt ggggttacag tttgagcagg
3061 atatttggtc ctgtagtttg ctaacacacc ctgcagctcc aaaggttccc caccaacagc
3121 aaaaaaatga aaatttgacc cttgaatggg ttttccagca ccattttcat gagttttttg
3181 tgtccctgaa tgcaagttta acatagcagt taccccaata acctcagttt taacagtaac
3241 agcttcccac atcaaaatat ttccacaggt taagtcctca tttaaattag gcaaaggaat
3301 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
3361 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
3421 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
3481 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
3541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
3601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
3661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
3721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
3781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
3841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
3901 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
3961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
4021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
4081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
4141 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
4201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
4261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
4321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
4381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
4441 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
4501 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
4561 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
4621 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
4681 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
4741 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
4801 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
4861 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
4921 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
4981 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
5041 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
5101 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
5161 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
5221 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
5281 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
5341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
5401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
5461 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
5521 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
5581 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
5641 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
5701 gtactgagag tgcaccatat cgacgctctc ccttatgcga ctcctgcatt aggaagcagc
5761 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
5821 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
5881 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
5941 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatct
6001 ggctagcgat gaccctgctg attggttcgc tgaccatttc cggggtgcgg aacggcgtta
6061 ccagaaactc agaaggttcg tccaaccaaa ccgactctga cggcagttta cgagagagat
6121 gatagggtct gcttcagtaa gccagatgct acacaattag gcttgtacat attgtcgtta
6181 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
6241 tatagaatac
//