LOCUS LT727281 6628 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMxCAT(stop)hTNFR55i, complete sequence.
ACCESSION LT727281
VERSION LT727281.1
KEYWORDS .
SOURCE Vector pMxCAT(stop)hTNFR55i
ORGANISM Vector pMxCAT(stop)hTNFR55i
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6628)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6628
/organism="Vector pMxCAT(stop)hTNFR55i"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3906."
/db_xref="taxon:1945355"
regulatory 13..458
/note="Mx"
/regulatory_class="promoter"
CDS 515..1174
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL87981.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
misc_feature 1175..1186
/note="GGSG"
CDS 1187..1867
/transl_table=11
/note="TNFRi"
/protein_id="SJL87982.1"
/translation="MGLMYRYQRWKSKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSF
SPTPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAPRREVAPPYQGADPILATALAS
DPIPNPLQKWEDSAHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLE
LQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAAL
PPAPSLLR"
misc_feature 1868..2019
/note="3UTR"
CDS <2074..2148
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL87983.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 2149..2215
/note="intron of SV40 small t-antigen"
misc_feature 2216..2767
/note="3' UTR of SV40 small t-antigen"
regulatory 2768..2864
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2768..2864)
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(2865..3677)
/note="SV40 VP1 with internal deletion"
regulatory complement(3733..3816)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(3910..4310)
/note="pMB1 ori"
CDS complement(4851..5711)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87984.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 6606..6622
/note="SP6"
/regulatory_class="promoter"
BASE COUNT 1691 a 1656 c 1598 g 1683 t
ORIGIN
1 aagcttgggc tgcagtagca gtgctcagac ctctaaacct gtagtcacaa gggacccaga
61 agggctggca gggctcctca gtgattctgg tatggtttgt ctctgaccct tcctagtgca
121 gctggaagaa tccagtgaaa agagaaagtt agtctaggtt gtgttcaact gagaggaggt
181 caggctgaag gctcagcaca gagctggaag ggtcctggga aagcaggcag ccatctgaac
241 tggtgtccat actgcatgtg tcttggtggg ctgagcctac aatatcgatt tcccaacctc
301 agtaccaagc caaggcccct cagggtgagt ttcgtttctg agctcctttc atatgtggtg
361 ggggtgtggg cacagcaagc tcaggctttt tcacggtttc aattctcctc tggaaggggc
421 ggagccagca ctatttaaag aggtctgagc cctggcagga ctctagagga tctcgaggag
481 cttggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
541 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
601 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
661 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
721 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
781 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
841 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
901 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
961 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
1021 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
1081 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
1141 acagtactgc gatgagtggc agggcggggc gtaaggcgga tccggcatgg gtttaatgta
1201 tcgctaccaa cggtggaagt ccaagctcta ctccattgtt tgtgggaaat cgacacctga
1261 aaaagagggg gagcttgaag gaactactac taagcccctg gccccaaacc caagcttcag
1321 tcccactcca ggcttcaccc ccaccctggg cttcagtccc gtgcccagtt ccaccttcac
1381 ctccagctcc acctataccc ccggtgactg tcccaacttt gcggctcccc gcagagaggt
1441 ggcaccaccc tatcaggggg ctgaccccat ccttgcgaca gccctcgcct ccgaccccat
1501 ccccaacccc cttcagaagt gggaggacag cgcccacaag ccacagagcc tagacactga
1561 tgaccccgcg acgctgtacg ccgtggtgga gaacgtgccc ccgttgcgct ggaaggaatt
1621 cgtgcggcgc ctagggctga gcgaccacga gatcgatcgg ctggagctgc agaacgggcg
1681 ctgcctgcgc gaggcgcaat acagcatgct ggcgacctgg aggcggcgca cgccgcggcg
1741 cgaggccacg ctggagctgc tgggacgcgt gctccgcgac atggacctgc tgggctgcct
1801 ggaggacatc gaggaggcgc tttgcggccc cgccgccctc ccgcccgcgc ccagtcttct
1861 cagatgaggc tgcgcccctg cgggcagctc taaggaccgt cctgcgagat cgccttccaa
1921 ccccactttt ttctggaaag gaggggtcct gcaggggcaa gcaggagcta gcagccgcct
1981 acttggtgct aacccctcga tgtacatagc ttttctcagg gggatccccc ctaggctttt
2041 gcaaaaagct caagcttggc tctagagtcg acggatcttt gtgaaggaac cttacttctg
2101 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
2161 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
2221 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
2281 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
2341 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
2401 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
2461 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
2521 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
2581 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
2641 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
2701 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
2761 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
2821 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
2881 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcgatccc caggaagctc
2941 ctctgtgtcc tcataaaccc taacctcctc tacttgagag gacattccaa tcataggctg
3001 cccatccacc ctctgtgtcc tcctgttaat taggtcactt aacaaaaagg aaattgggta
3061 ggggtttttc acagaccgct ttctaagggt aattttaaaa tatctgggaa gtcccttcca
3121 ctgctgtgtt ccagaagtgt tggtaaacag cccacaaatg tcaacagcag aaacatacaa
3181 gctgtcagct ttgcacaagg gcccaacacc ctgctcatca agaagcactg tggttgctgt
3241 gttagtaatg tgcaaaacag gaggcacatt ttccccacct gtgtaggttc caaaatatct
3301 agtgttttca tttttacttg gatcaggaac ccagcactcc actggataag cattatcctt
3361 atccaaaaca gccttgtggt cagtgttcat ctgctgactg tcaactgtag cattttttgg
3421 ggttacagtt tgagcaggat atttggtcct gtagtttgct aacacaccct gcagctccaa
3481 aggttcccca ccaacagcaa aaaaatgaaa atttgaccct tgaatgggtt ttccagcacc
3541 attttcatga gttttttgtg tccctgaatg caagtttaac atagcagtta ccccaataac
3601 ctcagtttta acagtaacag cttcccacat caaaatattt ccacaggtta agtcctcatt
3661 taaattaggc aaaggaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
3721 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
3781 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
3841 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
3901 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
3961 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
4021 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
4081 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
4141 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
4201 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
4261 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
4321 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
4381 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
4441 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
4501 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
4561 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
4621 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
4681 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
4741 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
4801 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
4861 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
4921 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
4981 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
5041 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
5101 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
5161 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
5221 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
5281 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
5341 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
5401 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
5461 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
5521 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
5581 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
5641 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
5701 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
5761 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
5821 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
5881 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
5941 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
6001 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
6061 gcggcatcag agcagattgt actgagagtg caccatatcg acgctctccc ttatgcgact
6121 cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga
6181 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac
6241 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga
6301 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc
6361 gtccggcgta gaggatctgg ctagcgatga ccctgctgat tggttcgctg accatttccg
6421 gggtgcggaa cggcgttacc agaaactcag aaggttcgtc caaccaaacc gactctgacg
6481 gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac acaattaggc
6541 ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac
6601 atacgattta ggtgacacta tagaatac
//